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Preparation method for lactobacillus plantarum used for fat reducing

A technology of Lactobacillus plantarum and processed products, which is applied in the field of health food to achieve the effects of promoting fat hydrolysis, preventing absorption and accelerating metabolism in the body

Inactive Publication Date: 2020-06-05
新食代布丁(上海)智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no stable and efficient product that specifically utilizes probiotics to effectively reduce body fat and reduce body fat. Therefore, the development of a Lactobacillus plantarum for fat reduction has a good research prospect and application market

Method used

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  • Preparation method for lactobacillus plantarum used for fat reducing
  • Preparation method for lactobacillus plantarum used for fat reducing

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preparation example Construction

[0022] A preparation method for fat-reducing Lactobacillus plantarum, the preparation method comprising the steps of:

[0023] (1) Take the fish skin and wash it with PBS buffer solution with a pH value of 7.3 to 7.7 for 2 to 4 times, drain the water, cut it into strips with a length of 2 to 5 cm and a width of 0.8 to 1.5 cm, and put it into a freeze dryer. Freeze-dry at -10°C for 20-24 hours, take it out and crush it through an 80-mesh sieve, collect the sieved particles and add them to the impregnation solution at a mass ratio of 1:8-13, stir at 10-15°C at 300-500r / min for 10-12 hours, and then use Centrifuge at 4000-6000r / min for 6-10min, collect the centrifuge and add it to 5% acetic acid solution at a mass ratio of 1:7-11, and add enzyme with 4-8% of the centrifuge at a low temperature of 0°C Material, enzymatic hydrolysis time 44-48h, centrifuged at 5000-8000r / min for 7-12min, ultra-filtered, freeze-dried to obtain fish skin treatment;

[0024] (2) Take the fish skin tr...

Embodiment 1

[0035] A preparation method for fat-reducing Lactobacillus plantarum, the preparation method comprising the steps of:

[0036] (1) Take the fish skin and wash it twice with PBS buffer solution with a pH value of 7.3, drain the water, cut it into strips with a length of 2-5 cm and a width of 0.8-1.5 cm, put it into a freeze dryer and freeze it at -10 °C Dry for 20 hours, take out and crush through an 80-mesh sieve, collect the sieved particles and add the impregnation solution at a mass ratio of 1:8, stir at 10°C at 300r / min for 10h, then centrifuge at 4000r / min for 6min, collect the centrifuged matter at a mass ratio of 1: 7 Add acetic acid solution with a mass fraction of 5%, and at a low temperature of 0°C, add enzyme material with 4% centrifuge mass, enzymolysis time is 44h, centrifuge at 5000r / min for 7min, ultrafilter, and freeze-dry to obtain Fish skin treatment;

[0037] (2) Take the processed fish skin and add it to beef at a mass ratio of 1:6 to mix, put it into a gr...

Embodiment 2

[0048] A preparation method for fat-reducing Lactobacillus plantarum, the preparation method comprising the steps of:

[0049] (1) Take the fish skin and wash it 4 times with PBS buffer solution with a pH value of 7.7, drain the water, cut it into strips with a length of 2-5 cm and a width of 0.8-1.5 cm, put it into a freeze dryer and freeze it at -10 °C Dry for 24 hours, take out and crush through an 80-mesh sieve, collect the sieved particles and add them to the impregnation solution at a mass ratio of 1:13, stir at 15°C at 500r / min for 12h, then centrifuge at 6000r / min for 10min, collect the centrifuged matter at a mass ratio of 1: 11 Add acetic acid solution with a mass fraction of 5%, add enzyme material with 8% centrifuge mass at a low temperature of 0°C, enzymolysis time 48h, centrifuge at 8000r / min for 12min, ultrafilter, and freeze-dry to obtain Fish skin treatment;

[0050] (2) Take the fish skin treatment substance and add it to beef at a mass ratio of 1:12, put it...

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Abstract

The invention discloses a preparation method for lactobacillus plantarum used for fat reducing, and belongs to the field of health-care foods. The lactobacillus plantarum prepared by the preparation method can coordinate intestines and stomach and boost metabolism, and slimming-oriented bacteria communities are cultivated to promote fat hydrolysis and combustion, prevent absorption of carbohydrateand oil, and tighten skin to make the skin elastic; according to the preparation method, lactobacillus casei and bifidobacterium lactis are employed to assist propagation of lactobacillus plantarum,and good slimming-oriented bacteria communities are constructed, so that the weight losing effect is enhanced; by performing crushing, ultrasonic treatment, and then grinding treatment on shepherdspurse herbs and grape seeds, physical barriers of crude fiber raw materials can be partially broken and the granularity of cellulose is reduced, so that supply of saccharides and other nutrients in the fermentation process is increased; moreover, fiber being of a porous structure can also be used as a carrier of resident bacteria, and is continuously differentiated in the reproduction process of thelactobacillus plantarum, and differentiated small molecular fiber can also assist in clearing the intestinal tract to avoid that lipids stay too long, and the lactobacillus plantarum can cooperate with probiotics to reduce cholesterol and cholic acid.

Description

technical field [0001] The invention belongs to the field of health food, and in particular relates to a method for preparing Lactobacillus plantarum for fat reduction. Background technique [0002] With the development of the economy and the improvement of living standards, the diseases caused by obesity have been increasing day by day in the past 30 years, and have become a worldwide medical and public health problem. Obesity is regarded as a chronic disease characterized by excessive body fat, multi-etiology, and multiple diseases. It is related to cardiovascular and cerebrovascular diseases, hypertension, type 2 diabetes, nonalcoholic fatty liver, fatty closely related to metabolic disorders. However, the existing treatment methods for obesity are diet control, drug intervention and surgical treatment, which have disadvantages such as poor effect, great damage to the body, and serious rebound. Especially for some surgical treatment and long-term oral medication patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61P3/04C12R1/25C12R1/225C12R1/01
CPCA61P3/04C12N1/20
Inventor 何楠覃扬
Owner 新食代布丁(上海)智能科技有限公司
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