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Complex microbial agent for intensifying anaerobic degradation of butyric acid and construction method of complex microbial agent

A technology of compound bacterial agent and oxidative bacteria, which is applied in the direction of chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high quality requirements for engineering technology and management personnel, increased operational difficulty and operating costs, Weaken the degradation ability of VFAs and other issues, and achieve the effect of flexible operation, simple structure and rapid start-up

Active Publication Date: 2020-06-19
广东桑海环保有限公司
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Problems solved by technology

The above research results show that the abundance of mutualistic symbionts of short chain fatty acid-oxidation syntrophs (SFAS) and methanogens in the anaerobic system can be enhanced by adding SFAS and acetic acid-type methanogens. However, there are mainly the following challenges: the target functional bacteria through external dosing can not achieve the enrichment target in the process of competition with the indigenous flora, especially in the case of continuous feeding and discharging. The effect of flora; the method of placing functional microorganisms in the anaerobic system through continuous dosing or cell immobilization technology has achieved certain results, but this method increases the operational difficulty and operating cost of actual operation and has a great impact on engineering technology and The quality requirements of management personnel are high, and the feasibility of actual operation is poor; in addition, from the perspective of microbial isolation and metabolic pathways, there are currently few acid-resistant methanogens that can be isolated and cultured, and acid-resistant methanogens can be obtained even after low-pH domestication. Methanogens can strengthen the recovery of the acidification system, but they cannot fundamentally change the inherent physiological characteristics of methanogens, such as the growth generation time and slow metabolic rate, and the best carbon source for methanogens under acidic conditions Possibly transformed into methanol and thus weakened the ability to degrade VFAs

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  • Complex microbial agent for intensifying anaerobic degradation of butyric acid and construction method of complex microbial agent
  • Complex microbial agent for intensifying anaerobic degradation of butyric acid and construction method of complex microbial agent
  • Complex microbial agent for intensifying anaerobic degradation of butyric acid and construction method of complex microbial agent

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Embodiment 1

[0038] Example 1

[0039] This embodiment provides a composite bacterial agent that strengthens the anaerobic degradation of butyric acid and its construction method. The composite bacterial agent includes fatty acid oxidizing bacteria Syntrophomonas wolfei and electrogenic bacteria Geobactorsulfurreducens (hereinafter referred to as: G.sulfurreducen); the composite bacterial agent The build process of the agent is as follows:

[0040] (1) Activation and enrichment of G.sulfurreducen

[0041] G.sulfurreducens was purchased from the American Type Culture Collection (ATCC, number 51573). The components of the G.sulfurreducens medium used in this example mainly refer to the medium components recommended by ATCC, and are improved according to the actual situation of this experiment and relevant literature. The components are shown in Tables 1-3.

[0042] Table 1 Complete medium of G.sulfurreducens

[0043] Element content Element content sodium acetate 1....

Embodiment 2

[0069] According to the system of the present invention, the effects of different concentrations of butyric acid, different concentrations of ammonia nitrogen and different pH on the metabolic growth of mutual symbionts were studied respectively.

[0070] (1) To study the effects of different butyric acid concentrations (2000-4500mg / L) on the metabolic growth of mutual symbionts, the operation period is 19 days, and three groups are operated at the same time.

[0071] (2) To study the effects of different ammonia nitrogen concentrations (500-3000mg / L) on the metabolic growth of mutual symbionts, butyric acid was 1500mg / L, the operation period was 19 days, and three groups were operated at the same time.

[0072] (3) To study the effects of different pH (5.5-8.0) on the metabolic growth of mutual symbionts, butyric acid 1500mg / L, the operation cycle is 19 days, and three groups are operated at the same time.

[0073] In order to reduce the risk of bacterial contamination, the m...

Embodiment 3

[0078] Further study the application effect of the composite bacterial agent of the present invention in actual wastewater. The composite bacterial agent constructed under the optimum conditions in Example 2 was added to the sludge dehydration liquid anaerobic fermentation system and regulated according to the above-mentioned optimal factor range, the inoculum ratio of the composite bacterial agent was 20.5±5.5g VSS / L sewage . Measure the VFAs value before and after water out, the results are as follows Figure 5 .

[0079] Depend on Figure 5 The results showed that the VFAs of water decreased from 1013mgHAc / L to 245mgHAc / L, a decrease of 75.8%, among which butyric acid decreased by 88.3%, which was a significant decrease. It shows that the composite microbial agent of the present invention is better applied in the process of wastewater treatment.

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Abstract

The invention discloses a complex microbial agent for intensifying anaerobic degradation of butyric acid and a construction method of the complex microbial agent. The complex microbial agent comprisesa fatty acid oxidation bacterium Syntrophomonaswolfei and an electrogen bacterium Geobactorsulfurreducens. According to the construction method, the fatty acid oxidation bacterium Syntrophomonaswolfei and the electrogen bacterium Geobactorsulfurreducens are activated, an experiment mode that an electrode is inserted into an anaerobic bottle is adopted, an activation bacterium liquid is added intoan anode chamber, then a syntrophism symbiosis system for intensifying degradation of butyric acid is constructed, and the complex microbial agent can be applied to the sewage treatment process. Themethod has the advantages of being simple in structure, flexible to operate, rapid to start, stable in operation, free of aeration, low in operation cost, and thelike, and optimal conditions for degradation of butyric acid can be regulated and controlled through pH values, ammonia nitrogen and initial butyric acid concentration values.

Description

technical field [0001] The invention belongs to the technical field of anaerobic fermentation, and relates to a microbial battery, in particular to a composite bacterial agent for strengthening the anaerobic degradation of butyric acid and a construction method thereof. Background technique [0002] Anaerobic biotechnology is widely used to treat high-concentration organic wastewater and has achieved good practical results due to its ability to simultaneously reduce organic matter in wastewater and increase productivity. The basic principle is to realize the gradual degradation of organic matter and the production of methane through the synergistic effect of hydrolytic fermentation bacteria, acetogenic bacteria and methanogenic bacteria. One of the main challenges to the efficient and stable operation of conventional anaerobic processes is the acid inhibition problem caused by the accumulation of volatile fatty acids (Volatile Fatty Acids, VFAs) in the system. During the op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/01C02F101/32
CPCC02F3/28C02F3/34C02F3/348C02F2101/322C12N1/20
Inventor 汪涛匡彬朱葛夫郏建波刘敏超李玉瑛李冰杨涛张梦辰徐晓龙
Owner 广东桑海环保有限公司
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