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Apocynum venetum tea compound functional beverage capable of preventing and treating cardiovascular and cerebrovascular diseases by targeting vascular endothelial cells

A technology for vascular endothelial and cell damage, applied in the biological field, can solve the problems of incomplete technology, unknown mechanism of action and side effects, difficult administration methods, etc., achieving strong innovation, alleviating vascular endothelial cell damage, and avoiding local ROS damage. Effect

Inactive Publication Date: 2020-07-24
陕西哲联生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Currently published prevention and health care programs for relieving vascular endothelial cell damage generally have problems such as incomplete technology. For example, patents such as application number 201910015995.1 use RNA to alleviate vascular endothelial damage, which leads to difficulties in drug delivery; application numbers are 201811150537.0 and 201880008458.8 Patents such as 201911011122.X and other patents only propose possible drug targets for vascular endothelial cell damage, but there are no mature and effective drugs that can target this target; patents such as 201911011122. Extract, claiming to prevent vascular endothelial damage, but the mechanism of action and side effects are unknown
In conclusion, there is currently no safe and effective mature program for the prevention and treatment of cardiovascular diseases targeting the core target of vascular endothelial cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Evaluation of formulation effectiveness and safety based on high-throughput standardized cell experiments.

[0028] On the basis of previous work, using high-throughput standard cell experiments, the optimal doses of each component of the formula were screened out to form formula A: Apocynum tea (calculate the amount of stock solution based on the final concentration of total flavonoids as 10 mg / L), 5- Hydroxytryptophan (40mg / L), Dihydrolipoic Acid (20mg / L), Pantothenic Acid (2mg / L) and Niacin (4mg / L).

[0029] Based on the previous work of the present invention, the corresponding ratio concentration of each component in the selected formula A (mass concentration ratio is according to the above order: 20:10:1:2, the final concentration of 5-hydroxytryptophan in the culture solution 30 μmol / L according to the formula A prepared according to the above ratio is 1 U), the viability and ATP production of vascular endothelial cells were measured by absorbance method...

Embodiment 2

[0030] Example 2 ROS free radical scavenging effect experiment of epidermal stem cells.

[0031] Based on the ratio and final concentration units of formulation A in Example 1, the intracellular ROS elimination and damage effects were determined at an optimal concentration of 35 U. First, DCF probe was used to measure the relative concentration of intracellular ROS, and it was found that the A pretreatment group was 42.3% lower than the negative control group; secondly, the cell death rate was measured by propidium iodide staining flow cytometry, and it was found that the formula A pretreatment group had a mortality rate of 1.1 %, the negative control group was 5.4%, and the T test found that the difference was significant after 5 independent experiments. The above data shows that this formula A can reduce the level of intracellular ROS and reduce cell death, so it can effectively prevent the cell damage caused by ROS.

Embodiment 3

[0032] Example 3 A randomized double-blind controlled multi-center volunteer experiment of this formula at an optimal concentration.

[0033] From July 2018 to July 2019, in Beijing, Xi'an and Xianyang, a total of 150 volunteers were selected from each city, with an average age of 42.3 years, a male to female ratio of 0.875, and a body mass index of 22.3. Volunteers used externally the formula A prepared in Example 1, the marketed cardiovascular health products B and C for a total of 120 days (the dosage of formula A was given according to Example 1, and the health products B and C were taken according to the instructions) , using the principle of randomized double-blind controlled volunteer experiment.

[0034] The vascular endothelial injury markers of volunteers were measured: vWF, PAI-1, E-selectin, vascular cell adhesion molecule-1, thrombomodulin and tPA, and the above results were normalized to generate a vascular endothelial injury score. The results showed that formu...

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Abstract

The invention discloses an apocynum venetum tea compound functional beverage capable of preventing and treating cardiovascular and cerebrovascular diseases by targeting vascular endothelial cells. Theamount of a stock solution is calculated according to a final concentration of total flavonoids of 3-100 mg / L, and components of 5-hydroxytryptophan (with a final concentration of 4-200 mg / L), dihydrolipoic acid (with a final concentration of 4-100 mg / L), pantothenic acid (with a final concentration of 1-10 mg / L) and nicotinic acid (with a final concentration of 2-20 mg / L) are additionally added.The formula has a good synergistic effect, with NADPH oxidase inhibited by apocynum venetum tea, the nicotinic acid is added, thus reactive oxygen species (ROS) injuries are not liable to be generated, moreover, the situation that nitric oxide and ROS form active nitrogen to induce nitrative stress, so as to injure vascular endothelial cells is prevented, and by inducing a state of the endothelial cells with a high nitric oxide content and a low ROS content, the added components enhance a physiological effect of the apocynum venetum tea, and reduce side effects. Moreover, in the aspect of developing the formula, by means of high-throughput standardized cell experiments and double-blind randomized controlled multicenter volunteer trials, action ranges and toxicity thresholds of the components of the formula are precisely verified, and the formula has certain tolerance in dosage, and has very high safety, so that the formula is suitable for being used as a core formula for product development.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a compound functional drink of apocynum tea that targets vascular endothelial cells to prevent and treat cardiovascular and cerebrovascular diseases. Background technique [0002] Cardiovascular and cerebrovascular diseases are the primary cause of threats to the health of the modern population, and are characterized by high disability rates, recurrence rates, and high mortality rates. According to information released by the World Health Organization, coronary heart disease accounted for the top ten causes of death in the world in the past ten years. 12.9%, stroke accounted for 11.4%, and accounted for 24.3% of the causes of death in total; the data released by the National Health and Medical Commission of my country also showed that the death caused by cardiovascular and cerebrovascular diseases ranks first in the Chinese population, and with the high-fat diet of the people , the risk ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/24A61P9/00A61P39/06A23F3/34A61K31/405A61K31/385A61K31/197A61K31/455
CPCA23F3/34A61K31/197A61K31/385A61K31/405A61K31/455A61K36/24A61P9/00A61P39/06A61K2300/00
Inventor 贺小星王文娟
Owner 陕西哲联生物科技有限公司
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