Apocynum venetum tea compound functional beverage capable of preventing and treating cardiovascular and cerebrovascular diseases by targeting vascular endothelial cells
A technology for vascular endothelial and cell damage, applied in the biological field, can solve the problems of incomplete technology, unknown mechanism of action and side effects, difficult administration methods, etc., achieving strong innovation, alleviating vascular endothelial cell damage, and avoiding local ROS damage. Effect
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Embodiment 1
[0027] Example 1 Evaluation of formulation effectiveness and safety based on high-throughput standardized cell experiments.
[0028] On the basis of previous work, using high-throughput standard cell experiments, the optimal doses of each component of the formula were screened out to form formula A: Apocynum tea (calculate the amount of stock solution based on the final concentration of total flavonoids as 10 mg / L), 5- Hydroxytryptophan (40mg / L), Dihydrolipoic Acid (20mg / L), Pantothenic Acid (2mg / L) and Niacin (4mg / L).
[0029] Based on the previous work of the present invention, the corresponding ratio concentration of each component in the selected formula A (mass concentration ratio is according to the above order: 20:10:1:2, the final concentration of 5-hydroxytryptophan in the culture solution 30 μmol / L according to the formula A prepared according to the above ratio is 1 U), the viability and ATP production of vascular endothelial cells were measured by absorbance method...
Embodiment 2
[0030] Example 2 ROS free radical scavenging effect experiment of epidermal stem cells.
[0031] Based on the ratio and final concentration units of formulation A in Example 1, the intracellular ROS elimination and damage effects were determined at an optimal concentration of 35 U. First, DCF probe was used to measure the relative concentration of intracellular ROS, and it was found that the A pretreatment group was 42.3% lower than the negative control group; secondly, the cell death rate was measured by propidium iodide staining flow cytometry, and it was found that the formula A pretreatment group had a mortality rate of 1.1 %, the negative control group was 5.4%, and the T test found that the difference was significant after 5 independent experiments. The above data shows that this formula A can reduce the level of intracellular ROS and reduce cell death, so it can effectively prevent the cell damage caused by ROS.
Embodiment 3
[0032] Example 3 A randomized double-blind controlled multi-center volunteer experiment of this formula at an optimal concentration.
[0033] From July 2018 to July 2019, in Beijing, Xi'an and Xianyang, a total of 150 volunteers were selected from each city, with an average age of 42.3 years, a male to female ratio of 0.875, and a body mass index of 22.3. Volunteers used externally the formula A prepared in Example 1, the marketed cardiovascular health products B and C for a total of 120 days (the dosage of formula A was given according to Example 1, and the health products B and C were taken according to the instructions) , using the principle of randomized double-blind controlled volunteer experiment.
[0034] The vascular endothelial injury markers of volunteers were measured: vWF, PAI-1, E-selectin, vascular cell adhesion molecule-1, thrombomodulin and tPA, and the above results were normalized to generate a vascular endothelial injury score. The results showed that formu...
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