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Fluorescent silica nanoparticles with nano-hydrophobic cage structure, preparation method and application thereof

A silica and nanoparticle technology, applied in the field of biomedicine, can solve the problems of limiting biomedical applications, reducing the fluorescence intensity of fluorescent silica nanoparticles, photostability and photodynamic treatment effect, etc., to achieve optimal photostability , Enhance the effect of photodynamic therapy, the effect of high phototoxicity

Active Publication Date: 2022-05-24
DALIAN UNIV OF TECH
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Problems solved by technology

However, due to the hydrophilic nature and porous structure of nano-silica, water and oxygen molecules can permeate and diffuse deep inwards, causing organic dyes with significant solvation effects to be easily affected by external environmental factors of nanoparticles, which greatly reduces fluorescence. The fluorescence intensity, photostability and photodynamic therapeutic effect of silica nanoparticles limit their further biomedical applications

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  • Fluorescent silica nanoparticles with nano-hydrophobic cage structure, preparation method and application thereof
  • Fluorescent silica nanoparticles with nano-hydrophobic cage structure, preparation method and application thereof
  • Fluorescent silica nanoparticles with nano-hydrophobic cage structure, preparation method and application thereof

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preparation example Construction

[0039] The preparation method of fluorescent silica nanoparticles with nano-hydrophobic cage structure, see the preparation process figure 1 , including the following steps:

[0040] (1) Preparation of organic dye doping precursor: Dissolve organic dye fluorescein, rhodamine derivative or cyanine dye containing carboxyl group and 3-aminopropyltriethoxysilane in a molar ratio of 1:3 In anhydrous dimethylformamide, under nitrogen protection, 60 ℃, magnetic stirring reaction for 2 ~ 4h, precipitating and washing with 10mL ether, and drying to obtain organic dye doping precursor;

[0041] (2) Pre-hydrolysis to construct a nano-hydrophobic cage structure: 3.5g of Triton X-100, 4.4mL of n-octanol and 20mL of cyclohexane were sequentially mixed in a 100mL round-bottomed flask, and the rotating speed was adjusted to 350rpm and stirred at room temperature for 5-10min; The organic dye doping precursor prepared in step (1) is dissolved in ultrapure water, added dropwise to the above inv...

Embodiment 1

[0046] Organic dye doping precursor: The synthetic route of heptamethyl tetraaminocyanine dye precursor is:

[0047]

[0048] Dissolve 0.15 mmol of heptamethanine dye and 0.45 mmol of 3-aminopropyltriethoxysilane in 10 mL of anhydrous dimethylformamide, react under nitrogen protection, 60 ℃, magnetic stirring for 4 h, and precipitate with 10 mL of ether and washing and drying to obtain an organic dye-doped precursor.

Embodiment 2

[0050] Preparation of fluorescent silica nanoparticles FSNPs-1 with nano-hydrophobic cage structure.

[0051] (1) Mix 3.5g Triton X-100, 4.4mL n-octanol and 20mL cyclohexane in sequence in a 100mL round-bottomed flask, adjust the rotational speed to 350rpm, and stir the microemulsion system for 10min at room temperature;

[0052] (2) The organic dye-doped precursor prepared in Example 1 was dissolved in 1 mL of ultrapure water, and added dropwise to the above-mentioned inverse microemulsion system. Then, 200 μL of n-propyltriethoxysilane, a silane coupling agent for constructing a nano-hydrophobic cage structure, was added dropwise to the above system. After stirring at room temperature for 30 min, 100 μL of ammonia water was slowly added dropwise to the above system to start the hydrolysis polymerization reaction for pre-hydrolysis. , to construct a nano-hydrophobic cage structure;

[0053] (3) After 12 hours of reaction at room temperature, 200 μL of ethyl orthosilicate was...

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Abstract

The invention belongs to the technical field of biomedicine, and relates to a fluorescent silica nano-particle with a nano-hydrophobic cage structure, a preparation method and an application thereof. The organic fluorescent dye is covalently coated in the nano-hydrophobic cage structure encapsulated by the hydrophobic alkyl chain through the hydrolytic polymerization process of the silane coupling agent containing the hydrophobic alkyl chain. The fluorescent silica nanoparticles with a nano-hydrophobic cage structure prepared by the present invention have greatly improved its photophysical properties and long-term and stable imaging capabilities of cancer cells, and at the same time can achieve effective photodynamic therapy for cancer cells. The fields of imaging and cancer therapy have promising applications.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a type of fluorescent silica nanoparticles with a nano-hydrophobic cage structure, which can be applied to long-term and stable fluorescence imaging and photodynamic therapy of cancer cells. Background technique [0002] Optical imaging can characterize biological processes at the cellular and subcellular levels of intact organisms with the advantages of high spatial resolution, low biological toxicity, and non-invasiveness. By developing specific fluorescent materials, this technology has played a powerful role in the field of cancer diagnosis and treatment. In recent years, numerous fluorescent nanomaterials have been developed and applied in optical imaging, such as organic dye-doped nanoparticles, carbon dots, quantum dots, fluorescent proteins, polymer nanoparticles, etc. Therefore, in order to meet the development needs of optical imaging technology in the biomedical f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/59C09K11/06C09B23/10A61K41/00A61K31/695A61P35/00G01N21/64
CPCC09K11/592C09K11/06C09B23/105A61K41/0057A61K31/695A61P35/00G01N21/6486C09K2211/1029
Inventor 宋锋玲焦龙
Owner DALIAN UNIV OF TECH
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