Application of symbiotic fermentation product of hydrolyzed candida and Japanese sake yeast
The technology of sake yeast and candida yeast is applied to the fermentation raw materials of skin care products and the field of skin care products. , good anti-aging effect
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Embodiment 1
[0061] Embodiment 1 Candida hydrolyzate and sake yeast symbiotic fermentation
[0062] The formula of the basic culture medium for fermentation is: the culture medium contains beef extract 10g / L, tryptone 5g / L, casein 3g / L, glucose 5g / L, water-soluble starch 1g / L, potassium dihydrogen phosphate 1.5g / L , anhydrous sodium acetate 3g / L, the pH value is adjusted and controlled with acid or alkali, and the rest is water. Different amounts of culture fluid were reduced and enlarged according to the ratio of components.
[0063] All the embodiments of the present invention adopt the above-mentioned medium formula as the culture medium for strain fermentation. Of course, in actual industrial production, the proportioning can be carried out according to different requirements, and these formulas are common formulas for cultivating Japanese sake yeast and / or Candida hydrolyzate.
[0064] The strain preservation number of Japanese sake yeast is ATCC26422, which was purchased from the N...
Embodiment 2
[0079] Embodiment 2 Japanese sake yeast separate fermentation
[0080] The formula of the basic culture medium for fermentation is: the culture medium contains beef extract 10g / L, tryptone 5g / L, casein 3g / L, glucose 5g / L, water-soluble starch 1g / L, potassium dihydrogen phosphate 1.5g / L , anhydrous sodium acetate 3g / L, the pH value is adjusted and controlled with acid or alkali, and the rest is water. Different amounts of culture fluid were reduced and enlarged according to the ratio of components.
[0081] The strain preservation number of Japanese sake yeast is ATCC26422, and the No. 7 yeast was purchased from the Japan Brewing Association.
[0082] Step 1: Japanese sake yeast enrichment culture
[0083] (1) Take a ring of Japanese sake yeast ATCC26422 on the inclined surface of the test tube on the ultra-clean workbench, put it into a 250ml triangular flask containing 50ml of culture solution with a pH of 4.8±0.2, and connect 2 bottles in total, 200rpm, 25°C After culturi...
Embodiment 3
[0091] Embodiment 3 Candida hydrolyzate separate fermentation
[0092] The formula of the basic culture medium for fermentation is: the culture medium contains beef extract 10g / L, tryptone 5g / L, casein 3g / L, glucose 5g / L, water-soluble starch 1g / L, potassium dihydrogen phosphate 1.5g / L , anhydrous sodium acetate 3g / L, the pH value is adjusted and controlled with acid or alkali, and the rest is water. Different amounts of culture fluid were reduced and enlarged according to the ratio of components.
[0093] The species number of Candida hydrolyzate is CICC 31239, which was purchased from Shanghai Institute of Industrial Microbiology.
[0094] Step 1: Enrichment culture of Candida hydrolyzate
[0095] Take 2ml of Candida hydrolyzate CICC31239 cryopreservation solution on the ultra-clean workbench and insert it into a 2.5L Erlenmeyer flask filled with 2L of culture solution with a pH of 7.8±0.2. Cultivate at 150rpm at 25°C for 16h, and the bacteria are in the exponential growth...
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