Glutamate decarboxylase mutant and application thereof in preparation of gamma-aminobutyric acid

A technology of glutamic acid decarboxylase and glutamic acid oxidase, which is applied in the field of bioengineering, can solve the problems of unknown enzyme activity and achieve the effects of avoiding food safety hazards, high comprehensive utilization of raw materials, and reducing production costs
CN111635898AActive Publication Date: 2020-09-08TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
10 Cites 16 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2020-09-08

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a glutamate decarboxylase mutant with improved enzyme activity and application thereof. The glutamate decarboxylase coding gene from bacillus megatherium is subjected to site-specific mutagenesis, and the catalytic performance of the obtained mutant is obviously improved compared with that of a wild type mutant. A recombinant engineering strain is constructed on the basis of the mutant, gamma-aminobutyric acid is prepared by taking a large amount of fermentation product L-glutamic acid as a substrate through a whole-cell catalysis method, the maximum yield of gamma-aminobutyric acid reaches 625.6 g / L, the molar conversion rate in a catalytic system is close to 100%, and no by-product is produced. The glutamate decarboxylase mutant disclosed by the invention has a very wide application prospect in efficient production and preparation of gamma-aminobutyric acid.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a glutamic acid decarboxylase mutant and application thereof, belonging to the technical field of bioengineering. Background technique

[0002] γ-aminobutyric acid is a naturally occurring non-protein amino acid, which is a strong neuroinhibitory amino acid. It has physiological effects of sedation, hypnosis, anticonvulsion, and hypotension. It has a wide range of applications in the fields of food, medical care, and beverage processing. Application prospect and market demand. According to rough statistics, the current total production capacity of various types of GABA in the world is about 60,000 tons per year, and the total market value of GABA-added related products in my country is about 2.5 billion to 3 billion yuan, and the market is still in a period of slow growth. The preparation methods of γ-aminobutyric acid mainly include chemical synthesis, microbial fermentation and biotransformation, etc., but the chemical synth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More