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Collagenase inhibitor composition, anti-aging face cream, preparation method of anti-aging face cream

A technology of inhibitor and collagenase, which is applied in the field of daily chemicals, can solve the problems of high molecular weight of hydrolyzed collagen and difficulty reaching the dermis, and achieve the effect of mild formula composition and effective efficacy

Pending Publication Date: 2020-09-18
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the existing technology, the molecular weight of hydrolyzed collagen is too large, and it is difficult to penetrate the skin barrier of the human body to reach the dermis; hyaluronic acid cannot essentially slow down the loss of collagen; peptides and retinol have certain irritation and safety risks to the skin etc., the present invention firstly provides a collagenase inhibitor composition for inhibiting collagenase activity

Method used

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  • Collagenase inhibitor composition, anti-aging face cream, preparation method of anti-aging face cream
  • Collagenase inhibitor composition, anti-aging face cream, preparation method of anti-aging face cream
  • Collagenase inhibitor composition, anti-aging face cream, preparation method of anti-aging face cream

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0073] Dilute the licorice root extract with deionized water to obtain 5 groups of test solutions with different concentrations (final concentrations). The mass concentration of licorice root extract is shown in Table 1 below when performing the collagenase inhibition activity test.

[0074] Inhibition of collagenase activity test

[0075] Folin phenol reagent can be reduced by phenolic compounds to give blue color (molybdenum blue and tungsten blue mixture) under alkaline conditions. Because protein molecules contain phenolic amino acids (such as tyrosine, tryptophan, etc.), Make the protein and its hydrolysate undergo the above reaction, so this principle can be used to determine the protease activity. Usually casein is used as the substrate to react with the enzyme solution under certain pH and temperature conditions. After a period of time, the enzymatic reaction is terminated with trichloroacetic acid. The casein precipitate is removed by centrifugation or filtration and the ...

Embodiment 6-10

[0093] The licorice root extract was dissolved in 5 deionized water corresponding to the volume of Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When measuring the inhibition mechanism of inhibiting collagenase activity, the mass concentration of licorice root extract is shown in Table 2 below.

[0094] Determination of inhibitory mechanism of licorice root extract in inhibiting collagenase activity

[0095] Add 0.25mL of the test solutions of Comparative Example 1 and Examples 6-10 to 30 test tubes, and add 0.05ml, 0.1ml, 0.15ml, 0.2ml, 0.25ml collagenase (32U / mL), and all add pH 7.4 D-PBS buffer solution to a final volume of 0.5mL, then add 0.5mL (1.0%) of the substrate casein solution and mix it immediately. After incubating in a water bath at 37°C for 10 minutes, add 1mL (6.5%) of trichloroacetic acid solution and mix well. After standing for 10 minutes, centrifuge at 10000 rpm for 5 minutes to obtain the supernatant M of the experim...

Embodiment 11-15

[0109] The licorice root extract was dissolved in 5 deionized water corresponding to the volume of Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When measuring the inhibition mechanism of inhibiting collagenase activity, the mass concentration of licorice root extract is shown in Table 3 below.

[0110] Determination of inhibition type of licorice root extract on collagenase

[0111] Add 0.5 mL, 0.4 mL, 0.3 mL, 0.2 mL, and 0.1 mL of the substrate casein solution (1.0%) to 30 test tubes, and add Tris HCl buffer with pH 7.4 to a final volume of 0.5 mL, and then separately Add 0.25mL of the test solution of Comparative Example 2 and Examples 11-15, then add collagenase (32U / mL) and mix it immediately. After incubating in a 37°C water bath for 10 minutes, add 1mL (6.5%) of trichloroacetic acid solution and mix it evenly After standing for 10 minutes, centrifuge at 10000 rpm for 5 minutes to obtain the control supernatant m.

[0112] Add 0.5mL...

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Abstract

The invention discloses a collagenase inhibitor composition, an anti-aging face cream, a preparation method of the anti-aging face cream. The collagenase inhibitor composition comprises the followingcomponents in percentage by mass: 50-99% of licorice root extract and 1-50% of chamomile extract, wherein the mass ratio of the chamomile extract to the licorice root extract is preferably 1: 1-9. Theanti-aging face cream comprises a collagenase inhibitor composition and a penetration enhancer; and based on the total mass of the anti-aging face cream, the addition amount of the collagenase inhibitor composition is 0.01-10%, and the addition amount of the penetration enhancer is 0.01-10%. The collagenase inhibitor composition comprises 50-99% of licorice root extract and 1-50% of chamomile extract; and the penetration enhancer is bis-ethoxydiglycol cyclohexane 1,4-dicarboxylate. According to the collagenase inhibitor composition, the licorice root extract and the chamomile extract are matched, the synergistic interaction effect is achieved, and the activity of collagenase can be well inhibited. The anti-aging face cream can reduce degradation of collagen, has an anti-aging effect, hasmoisturizing and water locking functions, can stop water loss and creates a moisturizing barrier for skin.

Description

Technical field [0001] The invention relates to the field of daily chemicals, in particular to a collagenase inhibitor composition, an anti-aging cream and a preparation method thereof. Background technique [0002] Collagen is the oldest and most abundant extracellular matrix protein. It has a wide range of applications in food, cosmetics, pharmaceutical and biomedical industries. The collagen family of extracellular matrix proteins plays a crucial role in the evolution of multicellular animals. At present, 28 types of triple-helical proteins are named collagen, which can be divided into several subgroups according to their structural and functional properties. Collagen is mainly produced by fibroblasts in the dermis and is the main component of the dermis. It can maintain the structure of the skin and give it toughness and elasticity. The content and distribution of collagen determines the youthfulness of the skin. However, an abnormal decrease in collagen content will cause...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/37A61K36/484A61Q19/08A61P17/18
CPCA61K8/9789A61K8/37A61Q19/08A61K36/484A61K36/28A61P17/18A61K2800/5922A61K2800/782A61K2300/00
Inventor 谢佩佩杨登亮李传茂张楚标曾伟丹张伟杰
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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