Bacillus licheniformis and application thereof to aquiculture

A technology of Bacillus licheniformis and Bacillus subtilis, which is applied in application, Lactobacillus, Bifidobacterium and other directions, can solve problems such as limited effect, achieve the effect of improving immunity and disease resistance, and has wide application prospects

Active Publication Date: 2020-09-18
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] By adjusting the proportion of feed formula components and improving the breeding environm

Method used

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  • Bacillus licheniformis and application thereof to aquiculture
  • Bacillus licheniformis and application thereof to aquiculture
  • Bacillus licheniformis and application thereof to aquiculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation, screening and identification of embodiment 1 bacterial strain

[0019] 1. Sample

[0020] The intestines of crucian carp were collected from the freshwater aquaculture experimental field in Jiaonan City, Qingdao.

[0021] 2. Medium

[0022] 1) LB medium: 10g of peptone, 10g of sodium chloride, 1000ml of distilled water, pH7.0-7.2, prepare a solid medium, add 20g of agar, and sterilize at 121°C for 20min.

[0023] 2) Starch screening medium: 20g soluble starch, 1g potassium nitrate, 0.5g sodium chloride, 0.5g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.01g iron sulfate, 1000ml distilled water, pH7.0-7.2, prepare solid culture Add 20g of agar to the base, and sterilize at 121°C for 20min.

[0024] 3) Starch utilization medium: 10 g of soluble starch, 5 g of peptone, 10 g of sodium chloride, 1000 ml of distilled water, pH 7.0-7.2.

[0025] 4) Starch-bromocresol purple medium: 10 g of soluble starch, 5 g of peptone, 10 g of sodium chloride, 4 ml...

Embodiment 2

[0037] Embodiment 2 Bacillus licheniformis DN01 produces amylase ability determination

[0038] Inoculate activated Bacillus licheniformis DN01, take 5ml of the activated bacterial solution, inoculate it in a liquid fermentation medium, culture at 37°C, take samples every 8 hours, and measure the amylase enzyme in the fermentation supernatant according to the method of GB / T 24401-2009 live, expressed in U / ml. Amylase activity is defined as one enzyme activity unit (U) when 1 g of soluble starch is liquefied in 1 hour at pH 7.0 and 37°C. For the amylase production curve of Bacillus licheniformis DN01 see figure 1 .

[0039] From figure 1 It can be seen that the Bacillus licheniformis DNO1 provided by the present invention has a strong ability to produce amylase under liquid culture conditions. After 8 hours of cultivation, the activity of amylase produced by fermentation of the strain increased sharply. At 40 hours, the amylase activity reached 2417.5U / ml. Within the range ...

Embodiment 3

[0040] Embodiment 3 bacillus licheniformis DN01 degrades starch and produces short-chain fatty acid situation

[0041] The short-chain fatty acids produced by the decomposition of probiotics can affect the physiological function and growth of the body's intestinal tract. These short-chain fatty acids can provide energy for intestinal epithelial cells, maintain the water and electrolyte balance in the intestinal tract, and also maintain mucosal immune cells. , reduce the production of pro-inflammatory factors, which is beneficial to the repair of mucosal inflammation.

[0042]Bacillus licheniformis DN01 was inoculated in the starch utilization medium, cultured for 24 hours, the bacterial liquid sample was pre-cooled, acidified with concentrated sulfuric acid, extracted with ether, and the supernatant was used for sample detection. Gas chromatography test on the computer, the inlet temperature is 180°C, the column temperature is 80°C, the initial test time is 2min, then rise to ...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, in particular to novel bacillus licheniformis and an application thereof to aquiculture. The bacillus licheniformis is screened from intestines of crucian carps, the preservation No. is CCTCC NO:M2020090, starch can be efficiently utilized, the glycometabolism of a fish body can be improved, and reduction of the feed cost is facilitated. The bacillus licheniformis can notably restrain pathogenic bacteria, the immunity and the disease resistance of aquatic animals are improved, growth of the aquatic animals is promoted, and the bacillus licheniformis is widely applied to the field of aquatic product feeds.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening and application, and relates to a bacillus licheniformis and its application in aquaculture. Background technique [0002] As one of the three major nutrients, carbohydrates are the main source of energy for animals. Moreover, carbohydrates can be used as precursors for the synthesis of functional and structural macromolecules in the body. At the same time, they also affect the digestion and absorption functions of the intestinal tract of animals, and play a key role in the growth and development of animals. Appropriate carbohydrates in fish feed can not only improve the growth performance of fish, but also reduce the consumption of feed protein as energy, saving protein. With the increasing shortage of animal raw material resources such as fishmeal and fish oil, and the price rise, improving the utilization efficiency of feed raw materials has become an important resea...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A23K10/18A23K50/80C12R1/10C12R1/125C12R1/07C12R1/25C12R1/23C12R1/01C02F103/20
CPCC12N1/20C02F3/341A23K10/18A23K50/80C02F2103/20C12R2001/10C12N1/205A23V2400/113A23V2400/169A23V2400/51
Inventor 周怡郭本月刘广魏万权倪梦丽王磊
Owner 青岛玛斯特生物技术有限公司
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