Strain capable of producing nattokinase at high yield and method for preparing nattokinase product by using strain

A technology of nattokinase and production method, applied in the field of bioengineering, can solve problems such as low activity, and achieve the effects of improving liver function, reducing production cost and protecting activity

Active Publication Date: 2020-10-09
CHAMBROAD CHEM IND RES INST CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the activity of nattokinase products in China is generally relatively low. Screening strains with high activity nattokinase produc

Method used

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  • Strain capable of producing nattokinase at high yield and method for preparing nattokinase product by using strain
  • Strain capable of producing nattokinase at high yield and method for preparing nattokinase product by using strain
  • Strain capable of producing nattokinase at high yield and method for preparing nattokinase product by using strain

Examples

Experimental program
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Example Embodiment

[0045] Example 1

[0046] A kind of production method of nattokinase tablet, concrete steps comprise:

[0047] (1) Preparation of nattokinase fermented liquid:

[0048] On a sterile operating table, use an inoculation needle to scrape 1 ring of the activated test tube slant bacteria into 100ml LB medium, shake the temperature at 33°C, rotate at 190r / min, shake and cultivate for 24 hours, and stop the culture when OD600=0.6 , to obtain the seed solution; get the prepared seed solution and inoculate it into the sterilized fermentation medium according to the inoculum size of 1% (v / w) (the fermentation medium consists of glucose 1.5%, xylose 3%, soybean peptone 1% , Ammonium Sulfate 0.3%, Calcium Chloride 0.02%, Disodium Hydrogen Sulfate 0.13%, Sodium Dihydrogen Sulfate 0.05%, Ferrous Chloride 0.003%, Soybean Oil 0.7%, pH7.4), shaker temperature 37 ℃, speed 200r / min, shake culture for 32h, detect that the nattokinase content reaches 1214FU / mL, stop the fermentation, and obtain ...

Example Embodiment

[0054] Example 2

[0055] A kind of production method of high nattokinase activity natto, concrete steps comprise:

[0056] (1) Preparation of nattokinase fermented liquid:

[0057] On a sterile operating table, use an inoculation needle to scrape 1 ring of the activated test tube slant bacteria into 100ml LB medium, shake the temperature at 37°C, rotate at 160r / min, shake for 20 hours, and stop the cultivation when OD600=0.5 , to obtain the seed solution; get the prepared seed solution and inoculate it into the sterilized fermentation medium according to the inoculum size of 5% (v / w) (the fermentation medium consists of 2% glucose, 1% xylose, 1.3% soybean peptone , Ammonium Sulfate 0.8%, Calcium Chloride 0.03%, Disodium Hydrogen Sulfate 0.10%, Sodium Dihydrogen Sulfate 0.06%, Ferrous Chloride 0.005%, Soybean Oil 0.5%, pH7.3), shaker temperature 33 ℃, speed 180r / min, shake culture for 38h, detect that the nattokinase content reaches 1096FU / mL, stop the fermentation, and obta...

Example Embodiment

[0063] Example 3

[0064] A kind of production method of high nattokinase activity natto, concrete steps comprise:

[0065] (1) Preparation of nattokinase fermented liquid:

[0066] On a sterile operating table, use an inoculation needle to scrape 1 ring of the activated test tube slant bacteria into 100ml LB medium, shake the temperature of 35°C, rotate at 200r / min, shake and cultivate for 18h, and stop the culture when OD600=0.8 , to obtain the seed solution; get the prepared seed solution and inoculate it into the sterilized fermentation medium according to the inoculum size of 2% (v / w) (the fermentation medium consists of: glucose 0.8%, xylose 2%, soybean peptone 2% , ammonium sulfate 0.4%, calcium chloride 0.01%, disodium hydrogen sulfate 0.08%, sodium dihydrogen sulfate 0.08%, ferrous chloride 0.004%, soybean oil 1%, pH7.2), shaking table temperature 36 ℃, rotating speed 160r / min, shake culture for 40h, detect that the nattokinase content reaches 1053FU / mL, stop the fe...

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Abstract

The invention belongs to the technical field of bioengineering, and provides a strain with high yield of nattokinase and a method for preparing a nattokinase product by using the strain. The high-yield nattokinase strain is identified as a bacillus subtilis strain through 16SrDNA, the code of the strain is YJY20-05, the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.20017. When the strain is applied to nattokinase liquid fermentation, the enzyme activity is improved by one time and the time is shortened by one time compared with the original strain fermentation level; after nattokinase is extracted and purified, the nattokinase is firstly prepared into microcapsules and then compounded with lecithin to prepare the nattokinase tablets, the nattokinase tablets with the nattokinase content of 2000FU/tablet and the lecithin content of 0.3 g/tablet can be obtained, the activity of nattokinase is guaranteed, and the function of preventing and treating thrombotic diseases is enhanced.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and provides a high-yield nattokinase strain and a method for preparing nattokinase products using the strain. Background technique [0002] Thrombotic disease is generally a thrombus formed by the adhesion of platelets, fibrin, white blood cells and other substances in the blood. As the thrombus continues to grow, it causes blood vessel blockage or even closure, causing some organs of the body to malfunction due to ischemia. Various diseases of sexual disorders. Thrombotic disease has a high probability of disability and death, and is a disease that seriously endangers human health and life. According to reports, the number of patients with thrombotic diseases in China has reached more than 10 million, and there are about 1.5 to 2 million new patients every year, and more than 3 million people receive thrombosis treatment every year. With the advent of an aging society, it is estimated t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K38/48A61K9/20A61P7/02A61P1/16A61K31/685C12R1/125
CPCA61K38/482C12Y304/21062A61K31/685A61K9/2059A61P7/02A61P1/16C12R2001/125C12N1/205A61K2300/00
Inventor 潘冬梅蔡颖辉杨传伦王建平李超孟张心青王秀芝虞凤慧杨丹丹蔡倩倩
Owner CHAMBROAD CHEM IND RES INST CO LTD
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