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Biological filler embedded with anthraquinone and thiobacillus denitrificans, and sulfur autotrophic denitrification method

A technology of Thiobacillus denitrification and biological filler, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, biological treatment devices, etc., can solve the problems of low denitrification degradation efficiency and low mass transfer rate, and reduce waste water treatment. Cost, high water content, shortening the effect of hydraulic retention time

Pending Publication Date: 2020-11-17
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the invention scheme whose application number is CN201010565316.7 mentions that the quinone compounds 1,5-dichloroanthraquinone, 1,8-dichloroanthraquinone, etc. are immobilized and embedded for denitrification, but the scheme is limited to Embedding quinone compounds still has problems such as low mass transfer rate and low denitrification degradation efficiency in practical applications

Method used

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  • Biological filler embedded with anthraquinone and thiobacillus denitrificans, and sulfur autotrophic denitrification method
  • Biological filler embedded with anthraquinone and thiobacillus denitrificans, and sulfur autotrophic denitrification method
  • Biological filler embedded with anthraquinone and thiobacillus denitrificans, and sulfur autotrophic denitrification method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] In this embodiment, the preparation method of biological filler is as follows:

[0058] (1) First, inoculate Thiobacillus denitrificans into the enriched liquid medium for 18 hours, and the culture temperature is 28°C. After the cultivation is completed, the wet cells of Thiobacillus denitrificans are obtained after centrifugation in a high-speed centrifuge. The speed of the high-speed centrifuge was 3000 r / min, and the centrifugation time was 8 min.

[0059] The preparation method of the enriched liquid culture medium is as follows: dissolving 5.0 g of sodium thiosulfate pentahydrate, 2.0 g of potassium nitrate, 1.0 g of sodium bicarbonate, 1.0 g of potassium dihydrogen phosphate and 0.5 g of magnesium chloride hexahydrate in 1000 mL of deionized water. The medium was adjusted to pH 7.0 with sodium hydroxide, and sterilized at 121°C for 30 min to obtain an enriched liquid medium. The initial inoculum concentration of the Thiobacillus denitrificans in the enriched liqu...

Embodiment 2

[0073] In this embodiment, the preparation method of biological filler is as follows:

[0074] (1) First, inoculate Thiobacillus denitrificans into the enriched liquid medium for 20 hours at a temperature of 31°C. After the cultivation is completed, centrifuge with a high-speed centrifuge to obtain wet cells of Thiobacillus denitrificans. The speed of the high-speed centrifuge was 3000 r / min, and the centrifugation time was 8 min.

[0075] (2) adding polyvinyl alcohol and sodium alginate to water, heating it in a 99°C water bath to make it into a viscous liquid, cooling to 25°C, and then sterilizing at high temperature to obtain an embedding agent precursor, wherein polyvinyl alcohol (w / w) of 8% and 2% (w / w) of sodium alginate.

[0076] (3) Wash the scallop shell with tap water, dry it in the air, dry it at 95°C, wash it with hydrochloric acid with a mass fraction of 4% for 2 minutes, and grind it to 50 mesh. Dry the ground scallop shell powder in an oven at 125°C. After co...

Embodiment 3

[0085] In this embodiment, the preparation method of biological filler is as follows:

[0086] (1) First, inoculate Thiobacillus denitrificans into the enriched liquid medium for 24 hours, and the culture temperature is 30°C. After the cultivation is completed, the wet cells of Thiobacillus denitrificans are obtained after centrifugation in a high-speed centrifuge. The speed of the high-speed centrifuge was 3000 r / min, and the centrifugation time was 8 min.

[0087] (2) adding polyvinyl alcohol and sodium alginate to water, heating it in a 99°C water bath to make it into a viscous liquid, cooling to 25°C, and then sterilizing at high temperature to obtain an embedding agent precursor, wherein polyvinyl alcohol (w / w) of 9% and 3% (w / w) of sodium alginate.

[0088] (3) Wash the scallop shell with tap water, dry it in the air, wash it with hydrochloric acid with a mass fraction of 4% for 2 minutes after drying at 95° C., and grind it to 60 mesh. Dry the ground scallop shell pow...

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Abstract

The invention relates to a biological filler embedded with embedding anthraquinone and thiobacillus denitrificans. The biological filler comprises an embedding agent and an embedding material, whereinthe embedding material contains scallop shell powder, 1,5-dihydroxy anthraquinone and thiobacillus denitrificans, and the embedding agent comprises a polyvinyl alcohol sodium alginate blend membraneand a cross-linked composite membrane. According to the present invention, a quinone compound 1,5-dihydroxy anthraquinone is fixedly embedded to achieve the adjustment of the sulfur autotrophic denitrification rate, and the quinone compound, thiobacillus denitrificans and scallop shell powder with characteristics of good biocompatibility and low price are fixedly embedded together, such that the activity of microbial thalli can be improved so as to prevent the loss of the microbial thalli, the mass transfer rate can also be increased, and the denitrification reaction rate and conversion rate can be increased; and the invention also improves the embedding agent, and solves the technical problems existing in pure use of polyvinyl alcohol or sodium alginate for embedding in the prior art.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a biological filler and a sulfur autotrophic denitrification and denitrification method. Background technique [0002] Sulfur autotrophic denitrification utilizes Thiobacillus denitrificans, using elemental sulfur as an electron donor under anoxic conditions, NO 3 --N acts as an electron acceptor to realize the process of denitrification and denitrification. Compared with the traditional denitrification process using heterotrophic facultative anaerobic bacteria in the treatment of low C / N sewage, the method has the advantages of very little sludge production, no additional carbon source, and low operating cost. However, sulfur autotrophic denitrification technology also faces many new challenges, such as low efficiency, easy loss of bacteria, and sensitivity to changes in factors such as temperature and pH. [0003] The application of microbial embedding and immobi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/00C02F3/28C02F3/34C02F101/16
CPCC02F3/00C02F3/345C02F3/28C02F2203/006C02F2101/163C12N11/084C02F3/2806C02F3/348C02F3/305C02F3/327C02F3/341C02F2003/001C02F2203/00
Inventor 李再兴刘艳芳刘晓帅岳欣吕建伟吕永涛张凡韩学滨
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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