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Intelligent release-type protein metal ion antibacterial nanoparticle and preparation method thereof

A nanoparticle and metal ion technology, which is applied in the functions of food ingredients, food ingredients containing natural extracts, and food forming, etc., can solve the problems of limited antibacterial activity and difficult to control the release efficiency of nanoparticle-encapsulated essential oils, and achieve high targets. The effect of tropism, good product stability and special product structure

Active Publication Date: 2020-11-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the antibacterial activity of nanoparticle-encapsulated essential oils is limited due to the difficulty in controlling the release efficiency

Method used

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  • Intelligent release-type protein metal ion antibacterial nanoparticle and preparation method thereof
  • Intelligent release-type protein metal ion antibacterial nanoparticle and preparation method thereof
  • Intelligent release-type protein metal ion antibacterial nanoparticle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The bactericidal effect of embodiment 1 yarrow essential oil to escherichia coli O157:H7

[0019] 1 Experimental materials

[0020] Model strain: Escherichia coli O157:H7 (Escherichia coli O157:H7)

[0021] 2 Experimental methods

[0022] In phosphate buffered saline [Phosphate-Buffered Saline, PBS, 0.03mol / L, pH (7.2-7.4)] insert test bacterium Escherichia coli O157:H7 (10 5 -10 6 CFU / mL), add a certain concentration (2mg / mL, 4mg / mL) of yarrow essential oil, shake evenly, shake (120r / min) in a full-temperature air shaker at 37°C, and cultivate at 0h and 0.5h respectively. , 1h, 2h, 4h and 8h, take an appropriate amount of culture solution and carry out ten-fold serial dilution to 10 2 -10 3 CFU / mL, then pipette 100 μL of the diluted solution onto the solid medium, spread it evenly, and place it in a constant temperature and humidity incubator at 37°C for upside-down culture. After 48 hours, plate colonies were counted to evaluate the antibacterial activity of the...

Embodiment 2

[0025] Embodiment 2 Preparation of intelligent release protein metal ion antibacterial nanoparticles

[0026] 1 Experimental materials

[0027]

[0028] 2 Experimental methods

[0029] The preparation steps of intelligent release protein metal ion antibacterial nanoparticles are as follows:

[0030] ① Preparation of solvent phase: Dissolve 0.2 g of zein in 10 mL of 85% ethanol aqueous solution, stir magnetically for 12 h, and add yarrow essential oil (4 mg / mL) after completely dissolving.

[0031] ② Preparation of non-solvent phase: 30 mL polyether F68 aqueous solution (1.5%, w / v).

[0032] ③ The solvent phase was transferred to the non-solvent phase, homogenized at 3000r / min for 3min, and then evaporated in an exhaust hood for 15h to remove ethanol.

[0033] ④Chelation: adjust the pH of the protein solution to 12 with 0.2mol / L NaOH, add ZnSO 4 (1mmol / L), 25 ℃ water bath shaking table shaking reaction for 1h, and finally dialyzed in a dialysis bag for 48h to prepare ant...

Embodiment 3

[0034] Embodiment 3 Characterization of intelligent release type protein metal ion antibacterial nanoparticles

[0035] 1 Experimental materials

[0036] Intelligent release protein metal ion antibacterial nanoparticles

[0037] 2 experimental equipment

[0038] Laser Particle Size Analyzer Nano ZS90 UK Malvern Instruments Ltd.

[0039] Gas chromatography-mass spectrometry (GC-MS) 6890GC / 5973NMSD Agilent Corporation of America

[0040] 3 Experimental methods

[0041] ①Using a laser particle size analyzer (Nano ZS90) to measure the particle size, polydispersity index (PDI) and Zeta potential of intelligent release protein metal ion antibacterial nanoparticles.

[0042]②Using GC-MS to determine the encapsulation efficiency of yarrow essential oil in antibacterial nanoparticles. Gas chromatography (GC) conditions are: DB-WAX elastic quartz capillary column (0.25mm×60m, film thickness 0.25μm), solvent delay is 10min. The injection volume was 1.0 μL, the split ratio was 1:20;...

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Abstract

The invention belongs to the field of food antibacterial additives, and particularly relates to an intelligent release-type protein metal ion antibacterial nanoparticle and a preparation method thereof. The yarrow essential oil is wrapped in zein by adopting a nano precipitation method, the pH value of the solution is adjusted, so that the surface is negatively charged, and positively charged zincmetal ions are chelated through electrostatic interaction. The characteristics of bacterial metalloproteinase are utilized, the bacterial metalloproteinase is used as a biological switch, and when bacteria containing Zn<2+> metalloproteinase encounter chelated nanoparticles, the protease activity is activated, so that proteins on the surfaces of the nanoparticles are hydrolyzed, the wrapped yarrow essential oil is released, and the bacteria are killed. The novel antibacterial nanoparticle can effectively inhibit various bacteria such as escherichia coli O157:H7, staphylococcus aureus and Listeria monocytogenes, and can intelligently release an antibacterial agent.

Description

technical field [0001] The invention belongs to the field of food antibacterial additives, and in particular relates to an intelligent release type protein metal ion antibacterial nanoparticle and its preparation. Background technique [0002] Achillea essential oil, usually steam distilled from yarrow flowers, leaves, and stems, has been traditionally used as an anti-inflammatory for headaches and to control bleeding from wounds. However, due to the volatility and decomposability of yarrow essential oil under air, light and high temperature, its various industrial applications are limited. [0003] In recent years, nanoparticles, as drug delivery carriers, have attracted extensive attention from researchers due to their small particle size and large specific surface area. Zein, formerly known as rice protein, has an isoelectric point of 6.5 and is rich in glutamic acid (21%-26%), leucine (20%), proline (10%) and alanine ( 10%), hydrophobic amino acids and sulfur-containin...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23P10/30
CPCA23L33/105A23P10/30A23V2002/00A23V2200/30A23V2250/21A23V2250/548A23V2250/1642Y02A50/30
Inventor 崔海英李虹林琳柏梅戴锦铭
Owner JIANGSU UNIV
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