A kind of broad-spectrum antimicrobial peptide derived from Lactobacillus plantarum and its application
A technology of Lactobacillus plantarum and antimicrobial peptides, applied in the direction of Lactobacillus, applications, peptides, etc., can solve the problems of no antibacterial effect and limited application, and achieve the effect of inhibiting growth, reproduction and metabolic activities
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] The coryneform lactobacillus FZU122 in the present invention is isolated from sour cabbage made by rural households in Yushu City, Jilin Province.
[0026] The specific separation method is as follows: take 10 g of kimchi aseptically crushed, resuspend in sterile physiological saline, and after 10-fold gradient dilution, spread them on MRS solid medium plates respectively, and culture them statically at 37 °C for 18 h; A single colony was placed in MRS liquid medium, cultured statically at 37 °C for 24 hours, centrifuged to take the supernatant, and separated from Staphylococcus aureus and Salmonella typhimurium under the condition of removing the interference of organic acids, hydrogen peroxide and other substances. The antibacterial strains producing antibacterial substances were screened out by solid agar punching antibacterial method as indicator bacteria.
[0027] The screened bacterial strains producing antibacterial substances were Gram-positive, and the colonies...
Embodiment 2
[0036] Lactobacillus plantarum FZU122 was inoculated in MRS liquid medium, cultured and fermented at 37 °C for 24 h, and the supernatant was collected by centrifugation. Staphylococcus ATCC 43300, Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Escherichia coli 8099, Salmonella choleraesuis CICC 13312, Salmonella typhimurium CMCC 50115, Salmonella enteritidis CICC21527, Cronos sakazakii Bacillus ATCC 29544 and Salmonella choleraesuis ATCC 13312 were used as indicator bacteria, which were inoculated in LB solid medium, and the antibacterial effect of antibacterial agents on indicator bacteria was evaluated by punching antibacterial method. The result is as Figure 4 as shown, L. plantarum The supernatant of the fermentation broth of FZU 122 is effective against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Salmonella choleraesuis, Salmonella typhimurium, Salmonella enteritidis, Enterobacter sakazakii and other food-borne patho...
Embodiment 3
[0038] Lactobacillus plantarum FZU122 was inoculated in MRS liquid medium, cultured and fermented statically at 37 °C for 24 h, and the fermentation broth was centrifuged at 4 °C and 10,000 rpm for 10 min to obtain the supernatant of the original fermentation broth, and then use a rotary evaporator to Concentrate 8 times its volume at 45°C and refrigerate at 4°C for later use. Slowly add 95% absolute ethanol to the concentrated fermentation broth, and stir evenly until the final volume concentration of absolute ethanol is 75%, and stand at 4 °C for alcohol precipitation for 12 h. After alcohol precipitation, centrifuge at 10,000 rpm for 10 min at 4°C, discard the precipitate, and keep the supernatant. Rotary steam at 45 °C until no more liquid evaporates, and recover ethanol for repeated use. Store the samples in a refrigerator at 4°C for future use. Soak 50 g of Sephadex LH-20 dry powder in 65% ethanol for 24 h, fully swell, and fill it into a chromatography column. Use ul...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More