Preparation method of nitrocellulose chromatographic membrane for rapid immunoassay, nitrocellulose chromatographic membrane and application thereof

A nitrocellulose and rapid immunization technology, which is applied in the field of preparation of nitrocellulose chromatography membranes, can solve the problems affecting detection efficiency, poor hydrophilicity and uniformity of nitrocellulose membranes, etc., and achieves improved production efficiency, good hydrophilicity and the like. properties and isotropic uniformity, and the effect of improving hydrophilicity

Active Publication Date: 2021-07-23
浙江美易膜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the present invention provides a method for preparing a nitrocellulose chromatographic membrane for rapid immunoassay, which solves the problem in the prior art that the nitrocellulose membrane used for detection is poor in hydrophilicity and uniformity, which affects detection efficiency

Method used

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  • Preparation method of nitrocellulose chromatographic membrane for rapid immunoassay, nitrocellulose chromatographic membrane and application thereof
  • Preparation method of nitrocellulose chromatographic membrane for rapid immunoassay, nitrocellulose chromatographic membrane and application thereof
  • Preparation method of nitrocellulose chromatographic membrane for rapid immunoassay, nitrocellulose chromatographic membrane and application thereof

Examples

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preparation example Construction

[0028] The preparation method of the nitrocellulose chromatographic membrane that is used for rapid immunoassay, comprises the steps:

[0029] (1) Prepare casting solution: dissolve nitrocellulose, cellulose acetate and hydrophilic polymer polyethylene glycol and / or polyvinyl alcohol in a mixed solvent, then add surfactant, and finally add non-solvent water at 10 Stir evenly at a temperature of ~70°C, and after defoaming and filtering, the casting solution is obtained; first add a mixed solvent to fully dissolve nitrocellulose, cellulose acetate and hydrophilic polymers, and then add surfactants to assist in improving Hydrophilic;

[0030] Wherein, the mass ratio of cellulose acetate to nitrocellulose is 1:(10-10000), and in the casting solution, the parts by weight of each component are: 1-30 parts of nitrocellulose, 30-90 parts of mixed solvent 0.1-10 parts of hydrophilic polymer, 0.5-20 parts of non-solvent, 0.01-3 parts of surfactant;

[0031] The mixed solvent includes ...

Embodiment 1

[0042] This embodiment is a method for preparing a nitrocellulose chromatographic membrane for rapid immunoassay, comprising the following steps:

[0043] (1) prepare casting solution, casting solution is composed as follows:

[0044] Reagent weight percentage nitrocellulose 13.5％ Cellulose acetate 0.5％ polyvinyl alcohol 0.6％ Methanol 42.6％ propanol 13.6％ Ethyl acetate 13.2％ Sodium dodecyl sulfonate 0.1％ water 15.9％

[0045] Dissolve nitrocellulose, cellulose acetate polymer and polyvinyl alcohol in mixed solvent methanol, propanol, ethyl acetate, then add surfactant sodium dodecyl sulfonate, and finally add non-solvent water, at 30 Stir evenly under the temperature condition of ℃, after defoaming and filtering, the casting solution is obtained;

[0046] (2) Scratch film: coating the casting solution on a polyester backing material with a thickness of 100 μm, and the thickness of the coated wet film is 500 μm;

...

Embodiment 2

[0056] In this example, the preparation method of the nitrocellulose chromatographic membrane used for rapid immunoassay, the backing material in the film-forming formula is a polyethylene foam material with a thickness of 100 μm, and the composition of the casting solution is as follows:

[0057]

[0058]

[0059] Other conditions and parameters are the same as in Example 1.

[0060] The electron micrograph of the surface topography of the film obtained in this embodiment can be found in figure 2 , The performance test results of the film are shown in Table 1.

[0061] The structure of the film prepared in this embodiment is uniform, and the porosity is very high, see figure 2 , which shows a part of the membrane prepared in this example. The average pore diameter of the membrane prepared in this example is 7.7 μm and the surface contact angle is 65° through computer calculation combined with all pore diameters, indicating that the membrane product is very hydrophili...

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Abstract

The invention provides a method for preparing a nitrocellulose chromatographic membrane for rapid immunoassay, comprising the following steps: (1) preparing a casting solution: dissolving nitrocellulose, cellulose acetate and a hydrophilic polymer in a mixing Solvent, then add surfactant, finally add non-solvent and stir evenly, after defoaming and filtering, cast film solution; (2) scraping film: apply cast film solution on the base material, the wet film thickness of coating is 50-1000μm; (3) Film formation: Evaporate most of the solvent of the wet film after coating in a vacuum chamber, and then undergo a high-temperature non-solvent-induced phase inversion to form a film. The preparation method of the nitrocellulose chromatographic membrane for rapid immunoassay provided by the invention solves the problem in the prior art that the nitrocellulose membrane used for detection is poor in hydrophilicity and uniformity, which affects the detection efficiency.

Description

technical field [0001] The invention belongs to the technical field of rapid immunodetection, and in particular relates to a preparation method of a nitrocellulose chromatographic membrane for rapid immunodetection. Background technique [0002] In addition to conventional filtration and separation, nitrocellulose membranes are increasingly used for rapid biochemical detection. For example, in the colloidal gold-labeled immunoassay technology, the nitrocellulose membrane is the carrier of the C / T line, and it is also the place where the immune reaction occurs. Since the surface of colloidal gold is negatively charged, it can be labeled with positively charged antigens and antibodies on the surface through electrostatic interaction. Colloidal gold has a high electron density and can combine with a variety of biological macromolecules. It has become a non-radioactive tracer commonly used in immunolabeling techniques after fluorescein, radioisotopes and enzymes. This rapid de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08J5/18G01N33/558G01N33/569C08L1/18C08L1/12C08L71/02C08L29/04
CPCC08J5/18C08J9/28C08J2201/0502C08J2201/0504C08J2205/044C08J2301/18C08J2401/12C08J2429/04C08J2471/02G01N33/558G01N33/56983G01N2333/165
Inventor 韦江陈楚龙
Owner 浙江美易膜科技有限公司
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