Calcium phosphate and folic acid composite particles and preparation method and application thereof
A technology of calcium phosphate folic acid and composite particles, applied in phosphorus compounds, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problems of high price, oxidative damage, and not very good ultraviolet filters, and achieve high dispersion, The effect of good biocompatibility
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[0033] The preparation method of calcium phosphate folic acid composite particles according to one embodiment of the present invention comprises the following steps S1-S4:
[0034] S1, such as figure 1 As shown, polycarboxy polymers (such as PAA) and calcium sources (such as Ca(OH) 2 ) react in water to obtain carboxyl / calcium ion complex solution.
[0035] S2. Add a poor solvent (such as IPA) to the carboxyl group / calcium ion complex solution to obtain a cloudy solution.
[0036] S3, adding a phosphate solution to the turbid solution to obtain calcium phosphate nanoparticles (PAA-CaPNPs) with carboxyl groups on the surface.
[0037] S4. Using a carboxyl activator to activate the carboxyl groups on the surface of the calcium phosphate nanoparticles, and then adding folic acid solution for amidation reaction to obtain calcium phosphate folic acid composite particles.
[0038] Folic acid, also known as pteroylglutamic acid, is a water-soluble B vitamin composed of pterin, p-a...
Embodiment 1
[0056] Preparation of calcium phosphate nanoparticles under different reaction concentrations of embodiment 1
[0057] In Example 1, the ratio of calcium hydroxide-polyacrylic acid-phosphate was fixed, and the concentration of reactants was changed under the condition of fixed reaction volume, so as to investigate the particle size and morphology of calcium phosphate nanoparticles under different reaction concentrations.
[0058] (a) Weigh 106.5 mg, 213 mg, and 416 mg of calcium hydroxide, respectively, and prepare 50 mL of aqueous solution. And based on the quality of calcium hydroxide, polyacrylic acid (284mg, 568mg, 1109mg) was configured with a calcium hydroxide: polyacrylic acid mass ratio of 3:8, and dissolved in water to obtain a 50mL aqueous solution. Calcium hydroxide aqueous solution was added dropwise into polyacrylic acid aqueous solution to initiate acid-base neutralization reaction. After stirring and mixing, a stable carboxyl group / calcium ion complex is formed...
Embodiment 2
[0071] Preparation of calcium phosphate nanoparticles under different polyacrylic acid concentrations of embodiment 2
[0072] In Example 2, the reaction concentration of calcium and phosphorus was fixed, and the content of polyacrylic acid was changed, so as to investigate the particle size and morphology of calcium phosphate nanoparticles.
[0073] (a) Weigh 106.5 mg of calcium hydroxide and configure it into 50 mL of aqueous solution. And according to the quality of calcium hydroxide as a benchmark, the mass ratio of calcium hydroxide: polyacrylic acid is 3:8, 3:4, 3:2 to configure polyacrylic acid (284mg, 142mg, 71mg), dissolved in water to obtain 50 mL of aqueous solution. Calcium hydroxide aqueous solution was added dropwise into polyacrylic acid aqueous solution to initiate acid-base neutralization reaction. After stirring and mixing, a stable carboxyl group / calcium ion complex is formed to form a clear and transparent aqueous solution, and the total volume of the aqueo...
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