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A kind of bacillus licheniformis and use thereof

A technology of bacillus licheniformis and edible alkali, which is applied in the fields of fermentation engineering and enzyme engineering, can solve the problems of high product prices, harsh requirements, and high production costs, and achieve the effects of facilitating promotion, improving adsorption capacity, and reducing equipment investment

Active Publication Date: 2021-09-21
上海圣珑环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of bioactivated carbon from peanut shells requires harsh conditions, high production costs, and high product prices.

Method used

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  • A kind of bacillus licheniformis and use thereof
  • A kind of bacillus licheniformis and use thereof
  • A kind of bacillus licheniformis and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 This embodiment relates to an enlarged culture of Bacillus underclothes

[0034] First, strains expand the culture

[0035] 1. Save the strains: Save the tube slope.

[0036] 2, beveled seed culture

[0037] 1) Preparation of beveled medium: Dipassass of starch, corn, and magnesium hydrogen phosphate, magnesium sulfate, control starch, corn pulp, hydrogen phosphate, and magnesium sulfate, the mass fraction of magnesium sulfate is: 1%, 2%, respectively. 0.1%, 0.02%, with a hydroxide adjusted to be about 7, and the agar, 121 ° C, 30 min sterilized according to the weight of the solution. Another solution of sodium carbonate is 20% aqueous solution, which is equipped with 121 ° C, 30 minutes. After sterilization, the amount of sodium carbonate is mixed with other medium components after the end of the concentration of 1%, and the sodium carbonate is mixed with other medium components. Put into the sterile test tube, becomes a slope. After the inclined is solidifie...

Embodiment 2

[0048] EXAMPLES 2 This embodiment relates to a method of preparing a solid seed curve, and includes the steps of:

[0049] 1. Cultivation of Solid Seeds:

[0050] 1) The medium component:

[0051] A. Nutrient formulation: 2% sodium carbonate, 2 potassium hydrogen phosphate (chemical fertilizer), magnesium sulfate (chemical pure) 0.02%, prepared with tap water. Stir dissolve.

[0052] B. Solid seed curvature ingredients: peanut shell rolled down, add nutrient solution in 1: 1.2 ratio. Mix even in the stainless steel basin. 121 ° C, 30 minutes sterilization. That is, a solid seed curvature medium.

[0053] 2) Seedcam inoculation: Sterilized spectrum culture medium, in the seed culture chamber, the temperature of the material is cooled to 37-40 ° C in a 10 kg peanut shell, inoculated with 1 to 3 eggplant flasks, inoculated. Each eggplant bottle plus 50 ml of sterile water, scraped bacterial cells with a sterile squeezed knife, and completely dissipation. A small amount of material is...

Embodiment 3

[0056] Example 3 This example embodiment is directed to a fermentation peanut shells, peanut shells formulations prepared

[0057] 1, fermentation ingredients: Weigh crushed peanut shells 100kg.

[0058] Another sodium weighed 2.4kg, dipotassium phosphate 120g, magnesium sulfate 24g, add water 120kg. Stirred to dissolve. Add peanut shell, turned mixed uniformly. You can be vaccinated.

[0059] 2, inoculated fermentation materials: culture material with a good, solid seed song weighed 10kg, the dispersion material added in turn mixed uniformly.

[0060] 3, peanut shells fermentation

[0061] After inoculation of the fermentation material, before stacking, a thermometer plug, floating blanket plastic film, heat, moisture culture fermentation until the material temperature rises to 35-40 deg.] C, clawed paving, 10-20cm controlling material thickness, floating plastic blanket film, culture was continued. During the culture, the culture temperature in accordance with needs, a gap turne...

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Abstract

The invention provides a new application of bacillus licheniformis. Oxygen-containing groups have the ability to adsorb heavy metals. A pectinase-producing Bacillus licheniformis, numbered Bacillus licheniformis, ferments peanut shells to degrade the pectin on the peanut shells, and not only makes the wrapped, cellulose and hemicellulose, which have heavy metal adsorption capacity Oxygen-containing groups, unbound. Moreover, some metabolites with oxygen-containing groups will be produced during the microbial culture process. At the same time, the hydrolysis of pectin will make the structure of peanut shells looser and the adsorption surface area will increase, so many factors will improve the adsorption capacity. Thereby effectively improving the adsorption capacity of peanut shells to heavy metals.

Description

Technical field [0001] The invention is within the field of biotechnology, involving fermentation engineering and enzyme engineering. Background technique [0002] With the increasing development of industrialization, the heavy metal ion pollution of land is also more serious. At present, there are two main ways to control the governance of heavy metal pollution: [0003] 1. Change the presence of heavy metals, reduce its activity, make it passivated, detach from the food chain, and reduce its toxicity; [0004] 2, using special plants to absorb heavy metals in the soil, then remove the plant or use engineering techniques to change heavy metals to soluble, free state, then extract, then collect heavy metals in the collections, thereby rejuvenation of heavy metals and reduces Double purpose of heavy metal in soil. [0005] Two channels compare, the latter cost is high, and the cycle is longer, so the first approach is usually used. [0006] Methods for changing the presence of hea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/18C09K17/14C12R1/10
CPCC09K17/14C12N9/18C12Y301/01011
Inventor 张蔚郭伟伟罗远恒高俐张正瀚
Owner 上海圣珑环境科技有限公司
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