Culture medium for culture expanding and concentration of oocystis species
A culture medium and oocyst technology, applied in the direction of microorganism-based methods, single-cell algae, and adding compounds to stimulate growth, etc., can solve the problems of high algae mortality, insufficient growth speed, and insufficient production vigor of oocysts, etc. problem, to achieve the effect of improving growth activity, improving stimulation and promoting effect, and reducing the mortality of algae species
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Embodiment 1
[0023] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 50g / L, CH 4 ON 2 20g / L, sodium dihydrogen phosphate dihydrate 2g / L, ferric chloride hexahydrate 2g / L, cyclodextrin glucosyltransferase 2g / L, the B nutrient solution includes the following components and concentrations: citric acid 3g / L L. Ferric ammonium citrate 3g / L, calcium chloride dihydrate 20g / L, tetrahydrofurfuryl alcohol 3g / L, α-ionol 1g / L, boric acid 2g / L, MnCl 2 4H 2 O0.5g / L, ZnSO 4 ·7H 2 O0.2g / L, Na 2 MoO 4 2H 2 O0.3g / L, CuSO 4 ·5H 2 O 0.5g / L, Co(NO 3 )2·6H 2 O0.3g / L, vitamin B1 0.1g / L, vitamin B12 0.005g / L, biotin 0.003g / L.
[0024] As a further configuration of the present invention, the weight ratio of the A nutrient solution to the B nutrient solution in the culture medium for expanding an...
Embodiment 2
[0027] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 180g / L, CH 4 ON 2 20-50g / L, sodium dihydrogen phosphate dihydrate 6g / L, ferric chloride hexahydrate 3g / L, cyclodextrin glucosyltransferase 6g / L, the B nutrient solution includes the following components and concentrations: citric acid 8g / L, ferric ammonium citrate 8g / L, calcium chloride dihydrate 50g / L, tetrahydrofurfuryl alcohol 5g / L, α-ionol 5g / L, boric acid 3g / L, MnCl 2 4H 2 O 3g / L, ZnSO 4 ·7H 2 O 0.6g / L, Na 2 MoO 4 2H 2 O 0.8g / L, CuSO 4 ·5H 2 O 0.8g / L, Co(NO 3 )2·6H 2 O 0.6g / L, vitamin B1 0.3g / L, vitamin B12 0.001g / L, biotin 0.005g / L.
[0028] As a further configuration of the present invention, the weight ratio of the nutrient solution A and the nutrient solution B in the culture medium for expandi...
Embodiment 3
[0031] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 100g / L, CH 4 ON 2 35g / L, sodium dihydrogen phosphate dihydrate 4g / L, ferric chloride hexahydrate 2.5g / L, cyclodextrin glucosyltransferase 4g / L, the B nutrient solution includes the following components and concentrations: citric acid 5g / L, ferric ammonium citrate 5g / L, calcium chloride dihydrate 35g / L, tetrahydrofurfuryl alcohol 4g / L, α-ionol 3g / L, boric acid 2.5g / L, MnCl 2 4H 2 O 2g / L, ZnSO 4 ·7H 2 O 0.4g / L, Na 2 MoO 4 2H 2 O 0.5g / L, CuSO 4 ·5H 2 O 0.6g / L, Co(NO 3 )2·6H 2 O 0.4g / L, vitamin B1 0.2g / L, vitamin B12 0.003g / L, biotin 0.004g / L.
[0032]As a further configuration of the present invention, the weight ratio of the A nutrient solution to the B nutrient solution in the culture medium for expandi...
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