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Paenibacillus polymyxa DX32 and application thereof in inhibiting pathogenic fungi of pestalotiopsis plants

A technology of Paenibacillus polymyxa and Pseudomonas spp., applied in the field of microorganisms, can solve the problem of no inhibition of Paenibacillus polymyxa, and achieve a good inhibitory effect

Inactive Publication Date: 2021-04-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paenibacillus polymyxa has not been found to have an inhibitory effect on the phytopathogenic fungus of the genus Discotrichum spp.

Method used

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  • Paenibacillus polymyxa DX32 and application thereof in inhibiting pathogenic fungi of pestalotiopsis plants
  • Paenibacillus polymyxa DX32 and application thereof in inhibiting pathogenic fungi of pestalotiopsis plants
  • Paenibacillus polymyxa DX32 and application thereof in inhibiting pathogenic fungi of pestalotiopsis plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This example illustrates the isolation and characterization of Paenibacillus polymyxa DX32.

[0022] In the Fritillaria plantation in Daxi Village, Pan'an County, Zhejiang Province, non-susceptible plants were selected from the high-incidence area of ​​Botrytis cinerea, uprooted and put into sampling paper bags. The paper bags were marked and brought back to the laboratory for isolation of plant endophytic fungi. Pick the fritillaria leaves, rinse them with sterile water, drain them, put them in 2% sodium hypochlorite solution for 3 minutes, take them out, wash them with sterile water for 3 times, and then dry them in a sterile environment. After 5 hours, dry the fritillary leaves Cut the leaves with sterilized scissors, about 2mm×2mm in size, pick them up on NA solid medium plates with tweezers, and cultivate them in the dark at 28°C in an incubator. After the colony grows, use an inoculation loop to dip the bacterial colony onto a new NA solid medium plate for streak ...

Embodiment 2

[0026] This example illustrates the determination of the physiological and biochemical characteristics of Paenibacillus polymyxa DX32.

[0027] Use BIOLOG GENIII (operated according to the kit instructions) to measure the physiological and biochemical characteristics indicators such as the utilization of the only carbon source. strain DX 32 See Table 1 for the sole carbon source utilization and chemical substance tolerance of BIOLOG GENIII plates.

[0028] Table 1 Physiological and biochemical characteristics of the DX32 strain, such as the utilization of the only carbon source, determined by the BIOLOG GENIII plate

[0029]

[0030]

[0031] Note: +, positive; -, negative; w, weakly positive.

[0032] As can be seen from the results in Table 1, the antagonistic strain DX of the present invention 32Dextran, D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-turanose, stachyose, D- raffinose, α-D-lactose, D-melibiose, β-Methyl-D glucoside, D-salicin, N-Acet...

Embodiment 3

[0034] This example illustrates the confrontation growth test between Paenibacillus polymyxa DX32 and the common pathogenic fungus of the genus Discotrichum spp.

[0035] Adopt confrontation growth method to measure the antagonistic strain DX of the present invention 32 Inhibitory effect on the common pathogenic fungus of the genus Discoides spp. Use a hole puncher to take a 5mm cake of the tested strain and place it on the 1 / 3 of the diameter of the PDA plate, pick DX 32 A single colony of the bacterial strain reaches the symmetrical 1 / 3 of the PDA plate containing the bacterial cake, and the DX is measured after 5 days of constant temperature cultivation at 28°C 32 The size of the inhibition zone between the test strain and the colony, and record the data, no DX 32 The PDA plate containing bacteria cake was used as blank control.

[0036] Table 2 bacterial strain DX32 of the present invention and the bacteriostatic zone size (mm) of confrontation growth of common Discotri...

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Abstract

The invention relates to a paenibacillus polymyxa DX32 strain. The preservation number of the paenibacillus polymyxa DX32 strain is CCTCC No: M 2020639. The invention also discloses an application of the paenibacillus polymyxa DX32 in inhibition of pathogenic fungi of a pestalotiopsis plant. The paenibacillus polymyxa DX32 disclosed by the invention can be used for effectively inhibiting pathogenic fungi of pestalotiopsis plants, such as pestalotiopsis microspora, pestalotiopsis theae, pestalotiopsis chamaeropis, pestalotiopsis versicolor and pestalotiopsis paeoniae, and has a relatively good inhibition effect on hypha and spore germination.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Paenibacillus polymyxa DX32 and the application thereof for inhibiting plant pathogenic fungi of the genus Discotrichum genus. Background technique [0002] Paenibacillus polymyxa has a certain control effect on various plant diseases such as plant verticillium wilt, chickpea wilt, rape rot, and black pine root rot. The U.S. Environmental Protection Agency (EPA) has listed it as one of the types of microorganisms that can be used commercially. Whether using HY96-2 (P.polymyxa) fermentation broth or the fine-grained agent (KDLD) made of HY96-2, stable control effects have been achieved in the greenhouse and in the field, and the performance of the KDLD fine-grained agent in the field effect is more prominent. Trichosporum is an anamorphic endophytic genus of Deuteromycetes, a well-known species of plant pathogens, and is closely related to the genera Truncatella and Seiri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/01
Inventor 谢昀烨王汉荣方丽武军王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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