Bacillus mucilaginosus and method for preparing bio-organic fertilizer through solid state fermentation of bacillus mucilaginosus

A colloidal bacillus and bio-organic fertilizer technology, applied in the field of agricultural microorganisms, can solve problems such as industry development obstacles, difficulty in concentration and drying, and viscous fermentation liquid, and achieve the effects of balancing the ecosystem, improving the environment, and maintaining fertility

Active Publication Date: 2021-04-20
山东千丰农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, because Bacillus coliformis has been fermented in the traditional process of liquid, the number of viable bacteria is too small and the rate of spores is low; Become an obstacle to the development of enterprises and industries
[0003] In addition, in the production of traditional bio-organic fertilizers, biological agents and organic fertilizers are two separate processes, and finally they are mixed to make fertilizer; during the composting process of organic fertilizers, a large amount of nitrogen loss will occur

Method used

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  • Bacillus mucilaginosus and method for preparing bio-organic fertilizer through solid state fermentation of bacillus mucilaginosus
  • Bacillus mucilaginosus and method for preparing bio-organic fertilizer through solid state fermentation of bacillus mucilaginosus
  • Bacillus mucilaginosus and method for preparing bio-organic fertilizer through solid state fermentation of bacillus mucilaginosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation of Potassium Solubilizing Bacteria

[0024] The separation process is usually the following steps:

[0025] The first step is to prepare Alexander Borov medium: its components include: Na 2 HPO 4 2g, FeCl 3 0.005g, MgSO4 7H 2 O 0.5g, CaCO 3 0.1g, 3g sucrose, 1g potassium feldspar powder (washed five times with deionized water), 1000mL distilled water, pH 7.0, 20g agar, sterilized at 121°C for 20min.

[0026] The second step is the primary screening of potassium-solubilizing bacteria: inoculate the enrichment on the solid medium of Alexander Borov medium and place it in a constant temperature incubator at 37°C for 10 days, and observe whether there is a transparent ring formed on the medium and its size. Measure the hydrolysis circle and the colony diameter with a ruler, and the relative size of the potassium-decomposing ability of the bacterial strain can be judged according to the diameter ratio of the hydrolysis circle and the colony circle. Pick t...

Embodiment 2

[0028] Molecular Identification of 16S rDNA Sequence of Strain M-1

[0029] Genomic DNA of strain QF-101 was extracted by the Chelex method, and the 16S rDNA gene of the genomic DNA of strain QF-101 was analyzed using 16S rDNA universal primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'). Long sequences were amplified by PCR. The PCR product was subjected to 1% agarose gel electrophoresis, and the target DNA fragment was recovered using the SanPrep Column DNA Gel Recovery Kit (SangonBiotech, Order NO.B518131) after the target band was cut. 3730xl DNA Analyzer (Applied Biosystems) was used for sequence analysis of DNA fragments, and the sequencing kit used BigDyeTerminator v3.1 (Applied Biosystems).

[0030] The bidirectional sequencing results were spliced ​​using SeqMan (DNASTAR Lasergene) to obtain a nearly full-length 16S rRNA gene sequence. Using EzBioCloud Identify service (https: / / www.ezbiocloud.net / identify) and NCBI Nucleotide BLAST (https:...

Embodiment 3

[0033] Qualitative Test of Potassium Solubilizing Ability of Strain M-1

[0034] Cultivate the culture medium of strain M-1, the volume of liquid in a 250mL Erlenmeyer flask is 50mL, cultivate for 48h, and use the fermentation broth sterilized at 121°C for 30min as a blank control. Weigh 12.5g of potassium feldspar powder, add it to the culture medium and the inactivated culture medium respectively, do 3 parallels for each treatment, culture at 37°C and 160rpm, and use potassium tetraphenylborate on the 5th and 10th day The content of soluble potassium was determined gravimetrically.

[0035] Table 1 Qualitative test of strain M-1's ability to decompose potassium

[0036] 5-day potassium solution (mg / L) 10-day potassium solution (mg / L) blank control 2.381 4.912 Strain M-1 30.735 58.975

[0037] The bacterial strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preser...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to bacillus mucilaginosus M-1 with an efficient potassium dissolving function and growth promoting capacity, the preservation number of the bacillus mucilaginosus M-1 is CGMCC (China General Microbiological Culture Collection Center) No.19428, and a method for preparing a bio-organic fertilizer by using the bacillus mucilaginosus M-1 through solid-state fermentation of the bacillus mucilaginosus M-1. The method for preparing the bio-organic fertilizer through solid state fermentation comprises the following steps: (1) activating slant seeds; (2) culturing liquid seeds; (3) pretreating a substrate; and (4) carrying out solid-state fermentation culture. According to the method, the problems of thick fermentation liquor, low viable count and low spore rate of traditional bacillus mucilaginosus liquid fermentation are solved, the solid-state fermentation method is adopted, the cow dung is used as a substrate for simultaneous fermentation production of the bacillus mucilaginosus and secondary decomposition of the organic fertilizer are performed, the bio-organic fertilizer is finally formed, and the process is simple and easy to implement.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a colloidal bacillus and a method for preparing bio-organic fertilizers by solid-state fermentation thereof. Background technique [0002] Colloidal Bacillus (also known as jelly-like Bacillus) can decompose potassium and silicon in minerals such as feldspar and mica, promote the conversion of soil phosphorus and potassium, increase the supply of soil phosphorus and potassium, and increase crop yield. One of the microbial fertilizer strains with the largest application area, the longest production time and the most production enterprises. On the one hand, because Bacillus coliformis has been fermented in the traditional process of liquid, the number of viable bacteria is too small and the rate of spores is low; Become an obstacle to the development of enterprises and industries. [0003] In addition, in the production of traditional bio-organic f...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/20C05G3/00C05G3/60C05G3/80C12R1/07
CPCY02W30/40
Inventor 柳建学张芳芳张继田张建鑫
Owner 山东千丰农业科技有限公司
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