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Application of oral fullerene material in preparation of medicine for preventing and/or treating myocardial ischemia-reperfusion injury or ischemic heart disease

A technology for reperfusion injury and myocardial ischemia, applied in the field of biomedicine, can solve the problems of low clinical accessibility and difficult operation, and achieve the effect of high clinical accessibility, good biocompatibility and remarkable curative effect

Active Publication Date: 2021-05-04
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its effect on myocardial ischemia-reperfusion injury is rarely reported, and in this report, the fullerenol / alginate hydrogel was directly injected into the myocardial infarction area for treatment, which is difficult to operate and clinically feasible. low reach

Method used

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  • Application of oral fullerene material in preparation of medicine for preventing and/or treating myocardial ischemia-reperfusion injury or ischemic heart disease
  • Application of oral fullerene material in preparation of medicine for preventing and/or treating myocardial ischemia-reperfusion injury or ischemic heart disease
  • Application of oral fullerene material in preparation of medicine for preventing and/or treating myocardial ischemia-reperfusion injury or ischemic heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1: the preparation of fullerene material

[0079] Take an appropriate amount of fullerene C60 powder with a particle size of 2-3 μm, mix it evenly with pharmaceutical excipients such as microcrystalline cellulose, copovidone, glyceryl behenate, etc., and press it into tablets of 200 mg each with a tablet machine.

[0080] When used in animal experiments, the tablet is dissolved in purified water to form a suspension for use.

Embodiment 2

[0081] Embodiment 2: Particle size characterization of fullerene materials

[0082] Take an appropriate amount of the fullerene C60 material prepared in Example 1 and drop it on the cleaned silicon wafer. After the water is completely evaporated, observe the morphology and particle size of the C60 material with a scanning electron microscope SEM (Hitachi, S-4800, Japan). .

[0083] Experimental results such as figure 1 As shown, the particle size of the C60 material is 2-3 μm.

Embodiment 3

[0084] Example 3: Cytotoxicity and oxidative damage repair effect of fullerene materials on human umbilical vein endothelial cells HUVEC

[0085] 1. Cell culture conditions: HUVEC cells were cultured in a cell culture incubator (37°C, 5% carbon dioxide), the medium was MEM, and 10% FBS and 1% penicillin-streptomycin double antibody solution were added.

[0086] 2. Experimental methods and results:

[0087] (1) Use the WST-8 method to detect whether the fullerene material has cytotoxicity

[0088] Inoculate the HUVEC cell suspension with a density of 5×10^4 / ml in a 96-well plate at 200 μl / well. After culturing for 24 hours, suck out the supernatant and replace it with 200 μl of fullerene materials with different concentrations to make the final concentration 0 , 10, 20, 40, 60, 80, 100, 200, 400, 500μg / ml, 6 parallel experiments for each group, cultured for 24h. Add 100 μl of CCK-8 solution (diluted in colorless DMEM) to each well, and test the OD values ​​of different groups...

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Abstract

The invention provides application of an oral fullerene material in preparation of a medicine for preventing and / or treating myocardial ischemia-reperfusion injury or ischemic heart disease. The application is the application of the fullerene material in preparation of a medicine or a medicine carrier for preventing and / or treating at least one of the following diseases or conditions: 1) preventing and / or treating myocardial ischemia reperfusion injury or ischemic heart disease; 2) protecting myocardial cells; 3) inhibiting apoptosis of cells; 4) promoting oxidative damage repair of cells; 5) improving the structure and function of a heart; 6) relieving symptoms such as myocardial edema, bleeding and ultrastructure change caused by myocardial ischemia and the like; and 7) improving far-end tissue damage caused by myocardial ischemia.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of orally administered fullerene materials in the preparation of drugs for preventing and / or treating myocardial ischemia-reperfusion injury or ischemic heart disease. Background technique [0002] Cardiovascular disease is the disease with the highest mortality rate in the world, accounting for one-third of the total mortality, among which acute myocardial infarction is the most serious. There are more than 3 million patients with acute ST-segment elevation myocardial infarction every year, and the most effective treatment for these patients is timely and effective reperfusion therapy. The improvement of myocardial blood supply by reperfusion therapy is accompanied by a series of pathophysiological reactions, including peroxidation, inflammation, intracellular calcium overload, and finally irreversible apoptosis and necrosis. This kind of myocardial injury caused by reper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61P9/10A23L33/10
CPCA61K33/44A61K9/0053A61P9/10A23L33/10A23V2002/00A23V2200/326A23V2250/1576A23V2250/156A23V2250/1602A23V2250/164
Inventor 王春儒贾旺甄明明白春礼姜俐赜
Owner INST OF CHEM CHINESE ACAD OF SCI
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