Enterobacter cloacae strain and application thereof

A technology of Enterobacter cloacae and strains, applied in the field of microorganisms, can solve the problems of soil acidification, soil hardening and hardening, reduction of oxygen content, etc., and achieve the effects of promoting plant growth and development, promoting occurrence and elongation, and increasing yield

Active Publication Date: 2021-05-07
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term large-scale application of chemical fertilizers is likely to cause soil pollution. For example, large-scale application of physiological acid fertilizers such as potassium chloride is likely to affect the physical and chemical properties of the soil, change the pH value of the soil, and cause the soil to become acidic, H + and Al 3+ content increased, thus affecting the normal growth of plants; excessive application of chemical fertilizers can also easily lead to Cl - , NO3 - And the accumulation of heavy metal ions in the soil will affect the quality of crops, which is not in line with people's pursuit of green organic food; in addition, the unreasonable application of chemical fertilizers will reduce the organic matter content of the farmland plow layer, destroy the aggregate structure, and reduce the water storage and fertilizer retention capacity. In addition to the effective use of fertilizers, it is easy to cause soil compaction and hardening, and the oxygen content is reduced, which in turn affects the development of plant roots.
Long-term application of chemical fertilizers not only pollutes and destroys the soil ecosystem, affects crop quality, but also seriously affects the water ecosystem
Research and surveys show that the chemical fertilizers used by plants only account for 30%-40% of the applied amount, and the remaining part is fixed in the soil on the one hand, and a large part enters the water body through seepage and leaching, resulting in the enrichment of the water body. Nutritization, causing algae and other plankton to multiply, reducing dissolved oxygen in water, deteriorating water quality, and causing mass death of fish and other organisms

Method used

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  • Enterobacter cloacae strain and application thereof
  • Enterobacter cloacae strain and application thereof
  • Enterobacter cloacae strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The source of embodiment 1, bacterial strain and separation, identification

[0025] The inventors of the present application isolated and obtained strain 49 from the rhizosphere soil microbial bacterial bank in Dongying Salt Land. By observing the morphological characteristics of strain 49, combined with its 16S rDNA sequence and homology analysis, it was identified that strain 49 was cloacae. Bacillus ( Enterobacter cloacae ); at the same time, its function in promoting plant root development and promoting plant growth and development was studied.

[0026] (1) Morphological observation of strain 49

[0027] Streak 49 on LB solid medium, culture at 30°C for 12-24 hours, observe the colony morphology and stain the strain 49 in the logarithmic growth phase with Gram staining method, and then observe Enterobacter cloacae strains through an optical microscope 49 morphological features.

[0028] It was found by observation that the single colony of bacterial strain 49 w...

Embodiment 2

[0036] Example 2, Experimental Research on the Promotion of Arabidopsis Lateral Root Development by Enterobacter cloacae Strain 49

[0037] The seeds of Arabidopsis thaliana were sterilized, cultured on the plate for 5 days, and transferred to a two-part petri dish, inoculated with Enterobacter cloacae strain 49, and cultured vertically. Half of the Arabidopsis seedlings in the two-part petri dish were the control (control), and the other half were Arabidopsis seedlings inoculated with strain 49, and the plant phenotype was observed after co-cultivation for 5 days. It was found that compared with the control, Arabidopsis thaliana seedlings exhibited a slightly shorter main root but significantly more lateral roots after being treated with strain 49, as shown in figure 1 As shown, it was shown that strain 49 could promote the development of Arabidopsis lateral roots.

[0038] Among them, the disinfection and plate culture method of Arabidopsis seeds is as follows: take a certa...

Embodiment 3

[0040] Example 3. Experimental research on bacterial strain 49 relying on tryptophan to synthesize auxin and activating the auxin signaling pathway

[0041] Transgenic seedlings capable of responding to auxin signal levels grown in 1 / 2 MS (with 1% sucrose, 0.8% agar powder) for 5 days DR5:GUS Transfer to a new 1 / 2 MS two-part solid medium (containing 0.8% agar powder) containing and not containing 50 μM tryptophan, and inoculate strain 49 to the plant root system, (the specific plant cultivation and inoculation methods are the same as in Example 2 ) After continuing to culture for 5 days, the plant root phenotype was observed, and it was found that the lateral root density of Arabidopsis thaliana was significantly increased after inoculating strain 49 compared with the control; especially on the medium containing tryptophan, after inoculating strain 49, Plants showed phenotypes similar to those treated with high-concentration auxin, that is, the main root was significantly sh...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to an enterobacter cloacae strain and application thereof. The enterobacter cloacae strain is preserved in the China General Microbiological Culture Collection Center on December 23 <rd>, 2020, and the preservation number is CGMCC NO. 21529. The enterobacter cloacae strain can promote plant root systems to synthesize auxin and promote plant lateral root development. Plant root system development promotion is specifically reflected in promoting generation and extension of plant lateral roots; plant growth and development promotion is specifically reflected in promoting increase of the fresh weight and yield of plants. The enterobacter cloacae strain can also be used for preparing auxin in a fermentation mode.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an Enterobacter cloacae strain and its application. Background technique [0002] With the growth of the world population, people have higher and higher requirements on the yield and quality of food and commercial crops. "Food is the most important thing for the people", the food problem has always existed and is an important problem to be solved. my country has a land area of ​​9.6 million square kilometers, ranking third in the world, with vast territory and abundant resources. However, due to the many mountains, deserts, and grasslands in the territory, the available arable land area is about 1.4 million square kilometers, accounting for about 16% of the country's land area. Due to factors such as desertification, conversion of farmland to forests, and urbanization of cultivated land in some areas of our country, the area of ​​cultivated land is actually d...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12P17/10C12R1/01
CPCC12N1/20A01N63/20C05F11/08C12P17/10Y02A40/10
Inventor 丁兆军张春雷张蒙悦郭中信耿进华
Owner SHANDONG UNIV
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