Anti-aging composition as well as preparation method and application thereof
A composition and anti-aging technology, applied in the field of daily skin care products, can solve problems such as polluting the environment, destroying land and water sources, and achieve the effect of inhibiting oxidation and improving the overall texture and appearance
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Embodiment 1
[0031] Embodiment 1 can effectively anti-aging preparation of plant extract composition
[0032] (1) After pulverizing the plant raw materials respectively, weigh them according to the proportioning ratio, and mix them evenly;
[0033] (2) Add deionized water for extraction, the mass ratio of plant raw materials to deionized water is 1:20-1:50, soak for 1-3 hours; add cellulase and pectin when the temperature is 40-50°C in water bath The mass ratio of enzyme, acid protease, cellulase, pectinase and acid protease is 1-3:1-3:1-3, and the total mass of added enzyme accounts for 10%-20% of the total mass of plant raw materials %, stirred for 10-30 minutes, and extracted by reflux in a water bath for 1-3 hours when the temperature was raised to 70-80°C;
[0034] (3) cooling the extract obtained in step (2) to 20-30° C., filtering with 100-200 mesh gauze to obtain the primary filtrate;
[0035] (4) adding clarifier to the initial filtrate obtained in step (3), centrifuging for 30-...
Embodiment 2
[0041] Example 2 Composite Antioxidant Efficacy Evaluation of Different Ratio Compositions
[0042] The three plant extracts were compounded according to the proportions in the following table to obtain compositions 1-15 and comparative examples 1-3.
[0043]
[0044]
[0045] (1) DPPH free radical scavenging test
[0046] Precisely pipette 2.0mL of 50% ethanol DPPH solution (0.2mmol / L), add 2.0mL of test solution, mix well and shake well, after 30min, measure its absorbance A at 517nm, measure in parallel 3 times, take the average value, Vitamin C was used as a positive control. Calculate the scavenging rate of each test solution to DPPH free radicals by the following formula:
[0047] DPPH clearance rate (%) = [1-(A i -A j ) / A 0 ]×100%
[0048] In the formula: A i is the absorbance of the reaction after adding the test substance;
[0049] A j is the absorbance of the test solution itself;
[0050] A 0 It is the blank value without adding test solution.
[005...
Embodiment 3
[0068] In a specific embodiment, the present invention provides a formula process of an effective anti-aging facial mask muscle base liquid, and the specific formula is as follows:
[0069]
[0070] Add the extract of the present invention, and add compositions 5, 11, and 13 into the formula of the lower surface membrane muscle base solution, and the addition amount is 1%, and the mask muscle base solution samples 1-3 are obtained.
[0071] Composition 5 Composition 11 Composition 13 sample 1 sample 2 sample 3
[0072] (1) MTT cytotoxicity test
[0073] According to 1×10 in 96well multi plate (corning) 4 DMEM medium containing 10% bovine serum and 100 μL of keratinocytes (HaCaT) were inoculated at the density of cells / well, and replaced with serum-free medium after 24 hours of culture. Compositions 5, 11, and 13 in the above examples were added to the serum-free medium for treatment and cultured for 24 hours. Thereafter, the medium was removed...
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