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Pseudomonas alcaligenes strain, biocontrol inoculant, and preparation method and use thereof

A technology of Pseudomonas alcaligenes and biocontrol agents, applied in biochemical equipment and methods, botany equipment and methods, and methods based on microorganisms, can solve tomato hazards, human and animal poisoning, and restrict the development of tomato production, etc. problem, to achieve the effect of simple production process, obvious antibacterial effect, and promotion of farmers' income

Inactive Publication Date: 2021-05-18
镇江敖来生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, tomato Phytophthora root rot has become one of the main diseases of tomato in winter and spring greenhouses. It can cause damage during the whole growth period of tomato. An effective method to prevent and control the epidemic of the disease and reduce losses
[0004] In the current production, chemical pesticides are mainly used to prevent and control tomato Phytophthora root rot; although this method is effective, the large-scale use and abuse of pesticides lead to frequent incidents such as environmental pollution, ecological damage, food pollution, and human and animal poisoning, and cause Serious "3R" (Residue, Resisitance and Resurgence) issues

Method used

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  • Pseudomonas alcaligenes strain, biocontrol inoculant, and preparation method and use thereof
  • Pseudomonas alcaligenes strain, biocontrol inoculant, and preparation method and use thereof
  • Pseudomonas alcaligenes strain, biocontrol inoculant, and preparation method and use thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Isolation, purification and identification of Pseudomonas alcaligenes Pas7

[0024] The Pseudomonas alcaligenes Pas7 for preventing and treating tomato root rot involved in the present invention was obtained in 2019 from the soil of a farm diseased field in Nancai Township, Sucheng District, Suqian City, Jiangsu Province;

[0025] Separation method: Use the 5-point sampling method to put the field soil into sterilized sealed plastic bags, and immediately bring it back to the laboratory; add 5g of the soil sample to 100mL of sterile water, and shake it on a shaker at 150r / min for 1 hour. Take it out and let it stand for 5 minutes, take 5mL of the supernatant and add it to 100mL of TMSM liquid medium, culture at 150r / min, 30°C for 24h on a shaker to activate the bacteria in the soil; draw 1mL of the culture solution to make a 10-fold gradient dilution, and take the gradient dilution Spread 0.1mL evenly on TMSM plate, cultivate and separate in 30℃ incubator. Se...

Embodiment 2

[0029] Embodiment 2: the preparation of biocontrol agent

[0030] Using the isolated Pseudomonas alcaligenes Pas7 to prepare a biocontrol agent, specifically includes the following steps: culture the Pseudomonas alcaligenes Pas7 in TMSM culture medium with shaking at 28°C and 180rpm for 20 hours to obtain seed liquid, seed liquid The total concentration of live bacteria in the medium is about 10 6 CFU / mL; inoculate the seed liquid with 1% inoculum in the fermentation culture liquid, fill in 30 mL (250 mL Erlenmeyer flask), and cultivate at 30 °C and 180 rpm to obtain a total concentration of viable bacteria of 1 × 10 9 CFU / mL finished bacterial agent.

[0031] TMSM culture solution: Add 1g L-tyrosine to 1000mL MSM, adjust the pH to 7.0, and add 1×10 5 Pa sterilization for 30min.

[0032] Fermentation medium: sucrose 2g / L, corn flour 15g / L, soybean flour 10g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 0.2g / L, yeast extract 0.4g / L, calcium chlorid...

Embodiment 3

[0033] Embodiment 3: Plate confrontation test detects the control effect of biocontrol agent to Phytophthora tomato

[0034] The biocontrol bacterium agent that embodiment 2 is made is diluted 5 times to obtain the total concentration of living bacteria to be 2 * 10 8 CFU / mL biocontrol agent, using mycelium growth rate method, transfer tomato root rot fungus (Phytophthora capsici) to PDA plate, activate at 25°C for 96h, and then use a puncher near the edge of the colony to prepare Bacteria cakes with a diameter of 5mm, and transferred to 0.1mL live bacteria with a total concentration of 2×10 8 The PDA plate of CFU / mL biocontrol agent was used as the treatment group, and the control group was directly transferred the tomato root rot bacteria to the PDA plate. Both the treatment group and the control group were cultured at 25°C. When the diameter is 4 / 5, the diameter of the colony is measured by the cross method, and the average value of the diameter of the colony is calculated...

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Abstract

The invention relates to a pseudomonas alcaligenes strain which is named as pseudomonas alcaligenes Pas7, and has a deposit date of November 9, 2020 and a deposit number of CGMCC NO.21151. The pseudomonas alcaligenes Pas7 has a simple production process, short culture time and low cost. Besides, the strain can be prepared into a biocontrol inoculant for preventing and treating tomato root rot through a simple, rapid and low-cost preparation method. Tests prove that the biocontrol inoculant has a prevention effect on the tomato root rot under a room temperature condition reaching 89.87%, and has a prevention effect on the tomato root rot in the field reaching 85.93%. The provided biocontrol inoculant is a microbial control agent and free of harms, does not cause environmental pollution, does not use other corresponding chemical pesticides or reduces dosage of them, saves expenditure of farmers and facilitates export of vegetables.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a Comamonas hydrochloride strain for preventing and treating tomato wilt, a biocontrol agent, a preparation method and application thereof. Background technique [0002] Tomato is one of the economic crops widely planted in the world. It is favored by growers and consumers for its variety, wide adaptability, high yield, rich nutrition, good edible quality and wide range of uses. It is widely distributed in different parts of the world. important vegetable crops in the region. Tomatoes are rich in vitamins, carbohydrates, minerals, organic acids and a small amount of protein. They can not only be eaten as fruits and vegetables, but also can be used as important processing raw materials, and have a wide range of uses in the field of consumption. However, various diseases of tomato have always been a major limiting factor of tomato yield and economic benefits. The dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P3/00A01P21/00A01G22/05A01G13/00A01C1/08C12R1/38
CPCC12N1/20A01N63/27A01G22/05A01G13/00A01C1/08
Inventor 张磊韩先旭李根郭威牛冬冬丁浩然
Owner 镇江敖来生物科技有限公司
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