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Activating agent for in-situ remediation of microorganisms in deep contaminated soil and application thereof

A technology of polluted soil and in-situ remediation, which is applied in the field of petroleum hydrocarbon polluted soil treatment and application, can solve the problems of loss of nutrients and microbial cells, uneven soil texture and structure, and low effective microbial biomass, etc. Sweep range, effect of increasing wetting performance

Active Publication Date: 2021-06-18
BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the bioremediation method is an effective treatment method that integrates low cost, environmental protection, and no secondary pollution. However, due to formation factors such as uneven soil texture and porosity, the adsorption capacity of added nutrients and microbial agents has a certain impact. Especially the sand layer structure with large porosity has poor water holding capacity, nutrients and microbial cells are easy to lose, and it is not easy to fix in the soil system and form biomass accumulation, resulting in low nutrient content and slow growth of bacteria , low effective microbial mass, long action cycle, etc.

Method used

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  • Activating agent for in-situ remediation of microorganisms in deep contaminated soil and application thereof
  • Activating agent for in-situ remediation of microorganisms in deep contaminated soil and application thereof
  • Activating agent for in-situ remediation of microorganisms in deep contaminated soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take the underground polluted soil of a petroleum and petrochemical enterprise, the average hydrocarbon concentration is 1260mg / m 3 , in a laboratory, soil microbial remediation experiment using a glass column setup.

[0032] The application method of this activator specifically comprises the following steps:

[0033] Step 1: Configuration of activator products

[0034] Firstly, according to the proportioning concentration of the activator product, prepare the activator as follows: rhamnolipid biosurfactant dry powder 8.2%, hydroxymethylcellulose 0.4%, ammonium chloride 25%, ammonium sulfate 15%, yeast extract powder 17% , dipotassium hydrogen phosphate 26%, hydrocarbon degrading bacteria powder 8.4%, mix uniformly in a mixer, and set aside;

[0035] Step 2: Calculation of Injection Volume

[0036] In an indoor environment at 15-18°C, a columnar glass soil treatment device was used to conduct a degradation experiment of petroleum hydrocarbons in soil. The bottom of ...

Embodiment 2

[0050] In situ soil microbial remediation treatment of a petroleum and petrochemical company, the average concentration of petroleum hydrocarbon pollutants in the treated soil was 740mg / m 3 .

[0051] The application method of this activator specifically comprises the following steps:

[0052] Step 1: Configuration of activator products

[0053] Firstly, according to the proportioning concentration of the activator product, prepare the activator as follows: rhamnolipid biosurfactant dry powder 8.5%, hydroxymethylcellulose 0.1%, ammonium chloride 24%, ammonium sulfate 16%, yeast extract powder 17% , 25.8% of dipotassium hydrogen phosphate, and 8.6% of hydrocarbon-degrading bacteria powder are mixed uniformly in a mixer. The mixed activator product is sealed and stored in sealed bags, and the package is 10 kg / bag.

[0054] Step 2: Calculation of Injection Volume

[0055] According to the previous field investigation results, the average thickness of the polluted layer is 3.7...

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Abstract

The invention discloses an activating agent for in-situ remediation of microorganisms in deep contaminated soil and application thereof. The activating agent is prepared from the following components in percentage by mass: 6 to 10 percent of rhamnolipid biosurfactant dry powder, 0.1 to 0.5 percent of carboxymethyl cellulose sodium, 10 to 20 percent of yeast extract powder, 20 to 30 percent of ammonium chloride, 10 to 20 percent of ammonium sulfate, 20 to 30 percent of dipotassium phosphate and 6 to 10 percent of hydrocarbon degrading bacterium agent. The activating agent has the beneficial effects that petroleum hydrocarbons on the surfaces of soil particles are combined by the surface activity of rhamnolipid, the hydrocarbon content in the soil water system is increased, and the contact probability of hydrocarbons and thalli is increased, so that the degradation efficiency is improved; the surface activity performance of rhamnolipid increases the wettability and the permeability of water in soil, nutritional agents and microorganisms can enter soil pores easily, the deep degradation efficiency of petroleum hydrocarbon in the soil is enhanced, the efficiency that petroleum hydrocarbon in the soil is utilized by the microorganisms can be improved, hydrocarbon degradation functional bacteria can be rapidly activated, and the treatment efficiency of petroleum hydrocarbon is enhanced.

Description

technical field [0001] The invention relates to an activator, in particular to an activator for in-situ restoration of microorganisms in deep polluted soil, and belongs to the technical field of oil hydrocarbon contaminated soil treatment application. Background technique [0002] At present, the treatment of petroleum hydrocarbon-contaminated soil has become a very important topic in the field of environmental protection. For the remediation of contaminated soil in some production enterprises, in-situ remediation methods are generally adopted, such as biological remediation, chemical oxidation, physical extraction, thermal desorption and other processes. Among them, the bioremediation method is an effective treatment method that integrates low cost, environmental protection, and no secondary pollution. However, due to formation factors such as uneven soil texture and porosity, the adsorption capacity of added nutrients and microbial agents has a certain impact. Especially ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40B09C1/10
CPCC09K17/40B09C1/10B09C2101/00
Inventor 寇焕起金艳方寇秋丽赵修全殷艺航
Owner BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV
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