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Compound microbial agent for sewage treatment and preparation method and application thereof

A compound microbial inoculum and sewage treatment technology, applied in microorganism-based methods, biochemical equipment and methods, biological water/sewage treatment, etc. and other problems, to achieve the effect of long survival time, improving growth and reproduction environment, and reducing BOD

Pending Publication Date: 2021-06-18
青海洁神环境科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial growth must meet a certain carbon-nitrogen ratio. When the carbon source in the sewage is insufficient or exhausted by microorganisms, or when the carbon source in the water is excessive, resulting in insufficient N, it will affect the growth and metabolic activities of microorganisms, and then Affect the effect of sewage treatment
In addition, due to the complex components of urban polluted water and the diversification of pollutants, it is difficult to achieve an effective purification effect for sewage treated by a single microbial strain.

Method used

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  • Compound microbial agent for sewage treatment and preparation method and application thereof
  • Compound microbial agent for sewage treatment and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0052] (1) Preparation of composite microbial agent

[0053] 1. Prepare culture medium

[0054] 1.1. Seed medium: 1% peptone, 0.5% yeast extract, 1% sodium chloride, natural pH;

[0055] 1.2. Basic fermentation medium: soybean cake powder 5%, corn starch 4%, ammonium sulfate 0.6%, K 2 HPO 4 1%, MgSO 4 ·7H 2 O 0.075%, NaCl 1.0%, pH7.8;

[0056] 2. Activation of strains: transfer the preserved strains to the slant medium for cultivation, and prepare them for later use;

[0057] 3. Preparation of seed solution

[0058] Take a ring of activated bacteria and insert it into a 250ml Erlenmeyer flask with a capacity of 50ml seed medium, at 37°C

[0059] Cultivate at 180r / min for 18h;

[0060] 4. Shake flask culture

[0061] Take 1ml of seed liquid respectively, put it into a 250ml Erlenmeyer flask filled with 50ml of fermentation medium, shake and cultivate at 30°C for 12h, and the rotation speed is 160r / min;

[0062] 5. Fermentation conditions

[0063] Use shake flasks to...

Embodiment 1

[0076] 1. Fermentation culture of each bacteria: Introduce the slant strains of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus lateralis and Thiobacillus denitrificans into LB liquid medium respectively, and culture them at 37°C; Insert the slant strain of Streptomyces griseus into the fermentation medium (soy bean cake powder 5%, cornstarch 4%, ammonium sulfate 0.6%, K 2 HPO 4 1%, MgSO 4 ·7H 2 O 0.075%, NaCl 1.0%, pH7.8 (with reference to Li Haiyan et al., Science and Technology and Engineering, 2013, 13 (27), 8202-8204)), 32 ℃ culture, until each bacteria in the liquid fermentation broth Bacteria concentrations were not less than 1×10 9 CFU / ml, freeze-dried to obtain dry bacteria powder in a dormant state.

[0077] 2. Preparation of modified highland barley stalk powder: take the highland barley stalk that has been dried and crushed and sieved through a 100-mesh sieve, add ammonia water with a concentration 14% (mass fraction) of 10 time...

Embodiment 2

[0081] In the same manner as in Example 1, dry powders of various bacteria in a dormant state were prepared, and the components were mixed according to the following ratios to obtain a composite microbial bacterial agent.

[0082] 15 parts of Bacillus subtilis, 15 parts of Bacillus licheniformis, 15 parts of Bacillus amyloliquefaciens, 15 parts of Bacillus lateralis, 13 parts of Streptomyces griseus, 8 parts of Thiobacillus denitrification, 75 parts of starch, 75 parts of modified highland barley straw powder .

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Abstract

The invention relates to a compound microbial agent for sewage treatment and preparation method and application thereof. The microbial agent is prepared from the following raw materials: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus laterosporus, streptomyces griseus and thiobacillus denitrificans, and optionally, weakly-alkaline modified straw powder. The preparation of the composite microbial inoculant comprises the following steps: preparing dry microbial powder in a dormant state, optionally adding the weakly-alkaline modified straw powder, and performing mixing according to a preset weight ratio to obtain the compound microbial agent. When being used for treating eutrophic sewage, the compound microbial agent has the following beneficial effects: as a dominant strain, the strain of the compound microbial agent has long lasting time, long survival time and high organic matter removal efficiency, and the preparation method is simple, the variety of required strains is few, the input cost is low, batch production can be achieved, and the compound microbial agent can be used for large-scale sewage treatment.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to a composite microbial bacterial agent for sewage treatment and a preparation method and application thereof. Background technique [0002] With the continuous advancement of the urbanization process and the increasing development of the industrial economy, there are more and more domestic sewage and industrial wastewater within the city, and the water quality is becoming more and more complicated and the pollution is becoming more and more serious. A large amount of domestic and industrial sewage flows into rivers and groundwater. Urban sewage not only contains organic pollutants, but also contains a large amount of suspended solids (including organic and inorganic substances), nitrogen and phosphorus pollution, etc. These pollutions are harmful to human daily life and human body. Health has a huge negative impact. Effective treatment and utilization of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/07C12R1/085C12R1/545C12R1/01
CPCC12N1/20C02F3/341
Inventor 柴建中柴伟贺
Owner 青海洁神环境科技股份有限公司
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