Method for improving yield of sucrose-6-acetate synthesized by enzyme means
An enzymatic synthesis and acetate technology, which is applied in the field of sweeteners, can solve problems such as low purification yield, complicated operation and post-processing, and excessive heavy metals in the product, so as to improve the reaction process, reduce the residual sugar content, and improve the reaction rate. Yield effect
Examples
preparation example 1
[0025] Resin pretreatment: Wash ECR-1030M lipase-immobilized resin (Hangzhou Chuangke Biotechnology Co., Ltd.) with 95% alcohol. After washing, wash with distilled water to remove ethanol, and obtain the pretreated resin by suction filtration.
[0026] Take 10L of deionized water, add disodium hydrogen phosphate and sodium dihydrogen phosphate to prepare a buffer solution with a pH of 7.5. Dissolve 50g (≥700u / mg enzyme activity) Candida lipase (Venoxin, Denmark) in the buffer, stir to dissolve the enzyme, and filter to remove insoluble components to obtain an enzyme solution. Add 180 g of pretreated resin to the enzyme solution, stir and adsorb at room temperature for 3 hours; the immobilization rate is 97.2% after testing. After the adsorption is completed, filter, and vacuum-dry the filter cake at 35°C until the water content is less than 5%, to obtain immobilized lipase.
Embodiment 1
[0028] Weigh 30g of sucrose into a four-necked flask, then pour 750ml of DMF into it, stir and dissolve in a 60°C water bath for about 40min. After the dissolution is complete, lower the temperature of the water bath to 35°C, pour 1000ml of tert-amyl alcohol into it, weigh and add 550g of vinyl acetate, stir and mix well, and when the temperature of the solution in the flask drops to 35°C, weigh and add 10g of Immobilized lipase, 35°C, 200r / min stirring reaction for 10h. After the reaction, filter with suction to remove the immobilized lipase and recover it. The filtrate is distilled under reduced pressure at 80° C. to remove the organic solvent at a pressure of -0.09 to -0.1 MPa to obtain sucrose-6-acetate. Sampling was carried out for liquid chromatography detection, and the calculated yield of sucrose-6-acetic acid was 89.8%.
Embodiment 2
[0030] Weigh 30g of sucrose into a four-neck flask, then pour 1000ml of DMF into it, stir and dissolve in a 50°C water bath for about 40min. After the dissolution is complete, lower the temperature of the water bath to 30°C, pour 1200ml of tert-amyl alcohol into it, weigh and add 625g of vinyl acetate, stir and mix well, and wait until the temperature of the solution in the flask drops to 35°C, weigh and add 15g of Immobilized lipase, 35°C, 200r / min stirring reaction for 10h. After the reaction, filter with suction to remove the immobilized lipase and recover it. The filtrate is distilled under reduced pressure at 85° C. to remove the organic solvent at a pressure of -0.09 to -0.1 MPa to obtain sucrose-6-acetate. Sampling was carried out for liquid chromatography detection, and the calculated yield of sucrose-6-acetic acid was 92.2%.
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Description
Claims
Application Information
- IPC
- C12P19/12
- CPC
- C12P19/12
- Inventors
- 庄勇; 陈文华