A kind of multifunctional anti-tumor nano drug and its preparation method and application
A nano-drug, anti-tumor technology, applied in the field of biomedicine
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Embodiment 1
[0033] Example 1 Preparation of amino-modified mesoporous Ag 2 S@SiO 2 core-shell nanoparticles
[0034] Using silicon dioxide (SiO 2 ) as carrier particles, using silver sulfide quantum dots (Ag 2 S QDs) as near-infrared second region fluorescent reagents and photothermal reagents, using n-tetradecanol as phase change material for coating. like figure 1 and figure 2 shown, the following are amino-modified mesoporous Ag 2 S@SiO 2 Specific preparation method of core-shell nanoparticles: Ag synthesis using thermal decomposition by existing methods 2 SQDs, thiol-terminated quantum dots were dispersed in chloroform and stored in a 4°C refrigerator for later use. Dissolve 0.05 g CTAB (hexadecyl triethyl ammonium bromide) in 2.5 mL deionized water, then add 0.25 mL Ag dispersed in chloroform 2 In S QDs, stir vigorously for 30 min to form a brownish yellow sol; put the sol in a 60 °C water bath and stir for 10 min to evaporate chloroform; then add 22.5 mL of deionized water...
Embodiment 2
[0035] Example 2 Preparation of multifunctional anti-tumor nanomedicine
[0036] Step 1. Amino-modified mesoporous Ag 2 S@SiO 2 Refer to Example 1 for the formation of core-shell nanoparticles.
[0037] Step 2. Folic acid (FA) activation: Dissolve 2.4 mg of FA in 10 mL of PBS solution (pH 5.6). Then 5 mg / 3 mg of EDS / NHS (carbodiimide / N-hydroxysuccinimide) was added to the above solution to obtain an activated FA solution.
[0038] Step 3. FA-modified AS nanoparticles: After the activated FA solution was reacted at room temperature for 30 minutes, the pH value of the FA solution was adjusted to 7.4 with NaOH, and 12 mg of AS NPs were added to the FA solution. After stirring at room temperature for 5 h, the mixture was centrifuged to obtain folic acid-conjugated ASF NPs (i.e., AS-FA NPs). AS nanoparticles are targeted by surface modification of folic acid.
[0039] Step 4. Preparation of DHA and SNP-loaded AS-FA nanoparticles: Disperse 2 mg AS-FA NPs in 2 mL ethanol, add 4 ...
Embodiment 3
[0041] Example 3 Application of multifunctional anti-tumor nanomedicine
[0042] The multifunctional Ag prepared in Example 2 2 S@SiO 2 -DHA / SNP / TD diagnosis and treatment probes can be used for photothermal / chemotherapy / gas therapy of tumors under near-infrared light irradiation on the one hand, and fluorescence and photoacoustic imaging in the second region of near-infrared on the other hand.
[0043] The following is an experiment to evaluate the combined therapeutic effect of artemisinin derivatives / sodium nitroprusside.
[0044] 1. Photothermal performance testing
[0045] To evaluate the photothermal effect, the photothermal effect was tested using solutions of AS NPs with different concentrations. 2 irradiated for 10 minutes. like image 3 As shown, the photothermal effect of AS NPs showed a clear concentration dependence, the temperature did not change significantly within 10 min without AS, while the photothermal effect of 70 °C could be achieved with the AS NPs....
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