Preparation method and application of polyphyllin nano-liposome dry powder inhalation

A technology of nano-liposome and polyphyll saponin, which is applied in the direction of liposome delivery, nanotechnology, nanotechnology, etc., can solve the problems of liposomes that are prone to oxidation, storage intolerance, and poor stability, and improve the therapeutic index , convenient transportation, and increased stability

Active Publication Date: 2021-11-26
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a saponin nano-liposome inhalation powder spray to solve the problems of poor stability in the above-mentioned prior art, liposomes are prone to oxidation and sedimentation, and are not resistant to storage

Method used

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  • Preparation method and application of polyphyllin nano-liposome dry powder inhalation
  • Preparation method and application of polyphyllin nano-liposome dry powder inhalation
  • Preparation method and application of polyphyllin nano-liposome dry powder inhalation

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Effect test

Embodiment 1

[0045] The preparation of embodiment 1 saponin VI nano liposome powder spray

[0046] Prepare papaya saponin liposomes by ethanol injection method, with a fixed drug-to-lipid ratio of 1:10, that is, take 270mg of hydrogenated soybean lecithin and 30mg of cholesterol dissolved in 10ml of absolute ethanol as the organic phase, take 30mg of papaya saponin VI, and 30mg of manna Alcohol was dissolved in 30 ml of phosphate buffer (pH 7.4, 0.01M) as the aqueous phase. Slowly add the organic phase to the water phase at a constant temperature of 50°C, stir for 10 minutes, mix evenly, and continue to stir until the ethanol is completely volatilized. The powder spray is prepared by spray drying. The specific process conditions are: Concentrate the heavy building with a rotary evaporator Saponin VI nano-liposomes, taking soluble solids as an index, are concentrated to the concentration (16wt%) of the concentrated solution required for spray drying. Based on 200ml of concentrated liquid, ...

Embodiment 2

[0049] The preparation of embodiment 2 saponins VI nano liposome powder spray

[0050] Prepare papaya saponin VI liposomes by solvent injection method: use ethyl acetate: ethanol (1: 1, v: v) as solvent, fixed drug lipid ratio is 1: 10 (mass ratio), hydrogenated soybean lecithin: cholesterol : Stearylamide (1: 1: 0.1, mass ratio) is used as lipid component, is dissolved in 5~10ml solvent as organic phase, gets 30mg pagoda saponin, and 30mg mannitol is dissolved in 30ml phosphate buffer (pH7. 4, 0.01M) as the aqueous phase. Slowly add the organic phase to the water phase whose temperature has been kept at 50°C, stir for 10 minutes, mix evenly, and continue stirring until the ethanol is completely volatilized, and then the nanoliposomes of papaya saponin VI are obtained, see image 3 ;Adopt the freeze-drying method to prepare the powder spray, and its process conditions are: use lactose and mannitol 1:1 (mass ratio) as the freeze-drying protective agent, use 10% as the drying a...

Embodiment 3

[0052] The preparation of embodiment 3 saponin VI nano liposomes

[0053] Prepare papaya saponin VI liposomes by ethanol injection method, that is, take 400mg hydrogenated soybean lecithin, 50mg cholesterol dissolved in 10ml absolute ethanol as the organic phase, take 50mg paprika saponin VI, 50mg mannitol and dissolve in 50ml phosphate buffer (pH7.4, 0.01M) as the aqueous phase. Slowly add the organic phase to the water phase whose temperature has been kept at 50°C, stir for 10 minutes, mix evenly, continue to stir until the ethanol is completely volatilized, and freeze-dry to obtain the nanoliposome powder of saponin VI precursor; use Box-Behnken The test optimized the main factors affecting the encapsulation efficiency of papaya saponin, the factors were ethanol quality (X1), hydrogenated soybean lecithin quality (X2), cholesterol quality (X3), and carried out the central combination design of factor level. Taking the encapsulation rate as an index, the experiment was arra...

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Abstract

The invention discloses a preparation method of a polyphyllin nano-liposome dry powder inhalation, which comprises the following steps: preparing liposome by adopting a film dispersion method, a reversed-phase evaporation method or a solvent injection method, and then drying by adopting a spray drying method or a freeze drying method to obtain the polyphyllin nano-liposome dry powder inhalation. The polyphyllin nano-liposome dry powder inhalation prepared by the invention has the advantages that the production conditions are easy to control, the particle size distribution of prepared particles is relatively concentrated, the stability, the flowability and the dispersity are good, the potential is 25-32mV, and the medicine can be slowly released within 16-24h. The polyphyllin nano-liposome dry powder inhalation can be used for preparing a medicine for preventing or treating 2019-nCoV(nCoV).

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a preparation method and application of papaya saponin nanoliposome powder spray. Background technique [0002] Paris polyphylla is a plant of the genus Paris Liliaceae (Liliaceae), also known as Zhaxiu, Caoheche, Baikansui, and its rhizome is called Chonglou. As a Chinese herbal medicine, Chonglou has a long history of medicinal use. Clinically, Chonglou is used internally for hemostasis, pain relief, asthma, cough, anti-tumor, etc.; externally used for anti-infection, anti-inflammatory and analgesic, etc., and folks are also used to treat various sores, carbuncles, snake bites, etc. , and studies have reported that Chonglou has an inhibitory effect on Asian influenza A virus (influenza Avirus, IAV). Scholars at home and abroad have carried out in-depth research on the chemical components of Chonglou, and have isolated and identified various chemical components su...

Claims

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Application Information

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IPC IPC(8): A61K9/72A61K31/7048A61K36/896A61K47/26A61P31/14B82Y5/00B82Y40/00
CPCA61K9/19A61K9/127A61K9/0073A61K31/7048A61K36/896A61K47/26A61P31/14B82Y5/00B82Y40/00Y02A50/30
Inventor 王纠王亚晶刘莹陈燕忠柏宁宁冯敏锭高颂邓启仪
Owner GUANGDONG PHARMA UNIV
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