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Brevibacillus fluminis strain and application thereof

A technology of brevibacillus and river water, applied in the field of microorganisms, can solve the problems of less research on biological functions, and achieve the effects of reducing nitrite, increasing safety, and reducing toxic effects

Active Publication Date: 2021-12-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the biological function of Brevibacillus fluminis
There is no report of Brevibacillus riparius that can degrade ammonia nitrogen and nitrite nitrogen

Method used

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  • Brevibacillus fluminis strain and application thereof
  • Brevibacillus fluminis strain and application thereof
  • Brevibacillus fluminis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Isolation, purification and screening of embodiment 1 bacterial strain

[0035] The inventor isolated and purified microorganisms capable of degrading ammonia nitrogen and nitrite from common wild rice in the wild rice core collection garden of South China Agricultural University. The specific separation and purification steps are as follows:

[0036] The medium used for separation is: NaCl 1g, MgCl 2 0.2g, NH 4 Cl 0.5g, CaCl 2 0.02g, K 2 HPO40.5g, KH 2 PO4 0.5g, distilled water 1000mL, pH natural (6.5), solid plus agar powder 18g / L, sterilized at 121°C for 30 minutes.

[0037] Crush the sample (common wild rice) with a sterilized calculus, weigh 10 g of the soil sample and suspend it with 90 mL of phosphate buffered saline (PBS) for 10 minutes, take 100 μL of the bacterial solution to coat the plate, and incubate in a 28-degree incubator. When using liquid medium for culture, use liquid medium (without adding agar powder), absorb 100 μL of bacterial solution for...

Embodiment 2

[0039] Physiological and biochemical characteristics and taxonomic status identification of the bacterial strain of embodiment 2

[0040] Identification and preservation of strains: the BF72 strain isolated in Example 1 has the following morphology and physiological and biochemical characteristics:

[0041] a. Morphological characteristics of the bacteria: the strain is short rod-shaped.

[0042] b. Colony morphological characteristics ( figure 1 ): On the LB agar plate, the growth rate is fast, the colony is round, white, with a raised center, and the edge is moist and neat (30°C, grow for 24 hours), the diameter of the colony is 1.8-2.0cm, and it produces oval spores. The optimum growth temperature is 30℃ and the optimum growth pH value is 7.

[0043] c. Physiological and biochemical characteristics: facultative aerobic, catalase positive, oxidase negative, able to utilize d-galactose, d-fructose, d-mannitol, N-acetylglucosamine, amygdalin, arbutin , salicylic acid, cello...

Embodiment 3

[0048] The carbon source utilization rate of embodiment 3 bacterial strains

[0049] A 96-well Bio-BIQA carbon source utilization plate was made with White liquid medium, and the carbon source utilization rate of strain BF72 was determined.

[0050] The formula is as follows:

[0051] (1) Each carbon source component with a final concentration of 0.1% (w / v) (see literature Peng Guixiang, Zhang Wu, Tan Weijun, Tan Zhiyuan. Development of rapid detection kits for bacterial physiological characteristics. Journal of Ecology, 2011, 30 (11 ):2509-2515.);

[0052] (2) Cs7 trace element mixture: MnSO 4 58 μM, H 3 BO 3 82 μM, ZnSO 4 3.5 μM, KI 6.0 μM, CuSO 4 0.8μM, Na 2 MoO 4 0.4 μM, CoCl 2 0.4 μM, FeSO 4 54 μM, EDTA-2Na 54 μM;

[0053] (3) White medium: A component: NaNO 3 2.5g, K 2 HPO 4 2.0g, NaCl 0.1g, CaCl 2 0.1g (separately sterilized), KH 2 PO4 1.0g, FeCl 3 0.01g, MgSO 4 ·7H 2 O 0.3g, agar 20g, distilled water 900mL;

[0054] Component B: Take 2.5mL...

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Abstract

The invention discloses a brevibacillus fluminis strain BF72 and an application of the brevibacillus fluminis strain BF72. The brevibacillus fluminis strain BF72 is preserved in Guangdong Province Microbial Culture Collection Center on April 13, 2021, and the preservation number of the strain is GDMCC No: 61567. The strain BF72 can utilize sodium lactate, sodium citrate and other specific carbon sources in the growth and metabolism process, and the utilization rate of the carbon sources is high. The strain can improve water pollution by reducing ammonia nitrogen, nitrite nitrogen and nitrite in a water body, can reduce toxic effects of nitrogen pollutants on aquatic animals and plants in the aspect of aquaculture, improves safety of aquatic food / feed / raw materials, and has a great application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Brevibacillus fluminis BF72 strain and application thereof. Background technique [0002] Ammonia nitrogen (including molecular NH 3 and ionic NH 4 + ), nitrite nitrogen (NO 2 - ), the concentration of ammonia nitrogen and nitrite nitrogen in water is an important indicator to measure the quality of water, ammonia nitrogen and nitrite nitrogen have strong toxicity to aquaculture animals, ammonia nitrogen has a great influence on the stability of aquatic animal cell membrane and the enzyme reaction in the body It will also affect the transfer of gills to oxygen, and cause damage to the excretory system and osmotic balance of aquatic animals. Molecular state in ammonia nitrogen (NH 3 ) and ionic state (NH 4 + ) can be transformed into each other in water, and NH 4 + compared to NH 3 Because of its high fat solubility, it can more easily diffuse into cells throug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/01C02F101/16
CPCC02F3/34C12N1/20C02F2101/16C02F2101/166Y02W10/10
Inventor 谭志远唐嘉城谭习羽张笑影
Owner SOUTH CHINA AGRI UNIV
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