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Ultrasonic integrated micro-droplet array detection platform and preparation method and application thereof

A detection platform and micro-droplet technology, applied in the preparation of test samples, biochemical equipment and methods, methods for stress-stimulated microbial growth, etc., can solve the problems of low sensitivity of new coronavirus, long detection cycle, and complex detection instruments , to achieve ultra-sensitive detection, good dispersion and mixing, and increase the effect of reaction rate

Pending Publication Date: 2021-12-17
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this application is to provide an ultrasonic integrated micro-droplet array detection platform and its preparation method and detection method, aiming to solve the problems of low sensitivity, long detection cycle and complex detection instruments in the prior art to a certain extent

Method used

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  • Ultrasonic integrated micro-droplet array detection platform and preparation method and application thereof
  • Ultrasonic integrated micro-droplet array detection platform and preparation method and application thereof
  • Ultrasonic integrated micro-droplet array detection platform and preparation method and application thereof

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preparation example Construction

[0057] The second aspect of the present application provides a method for preparing an integrated ultrasonic array droplet detection platform, comprising the steps of:

[0058] S1: Preparation of micro-column array, the array including a substrate and micro-pillars plurality of micro-pillars extending from a surface of the substrate;

[0059] S2: the piezoelectric transducer and the surface facing away from the micro-contact connected to the column substrate;

[0060] S3: The piezoelectric transducer is connected to the waveform generator, and such that the piezoelectric transducer capable of converting electrical signals into the waveform generator output ultrasonic wave.

[0061] Wherein, in step S1, the material is selected from the micro-pillar array polydimethylsiloxane (Polydimethylsiloxane, PDMS), polymethyl methacrylate (Polymethyl methacrylate, PMMA), polypropylene, polystyrene, polyvinyl chloride, acrylonitrile butadiene styrene polymers, styrene-butadiene copolymer and ...

Embodiment 1

[0081] The solution obtained by extracting the collected nasopharyngeal swab sample virus nucleic acid extraction kit was taken as a sample to be detected, and the RPA amplification reagent was added to the microcapper array, wherein the micromorridge diameter in the microcapper array was 2 mm. High 2mm, then the sample to be detected is added to the respective droplets in a certain order, and then the magnesium ion activator is added to the reaction system to activate the nucleic acid amplification reaction, after the magnesium ion, turn on the waveform generator power supply Open the ultrasonic 30S, so that the magnesium ions are fully mixed, then turn off the ultrasound, followed by ultrasound 30S, the frequency of 30s, the nucleic acid amplification reaction, the amplification condition room temperature 37 degrees Celsius, the blue flash flashlight and the mobile phone are detected. Record fluorescence intensity of different amplification time, such as Figure 4 As shown, the s...

Embodiment 2

[0083] The solution collected by the collected nasopharyngeal swab sample was extracted as a sample to be detected, and the RPA amplification reagent was added to the microcapper array, wherein the micromcompine diameter in the microcapper array was 0.5mm. 0.5mm high, then the sample to be detected is added to the respective droplets in a certain order, and then the magnesium ion activator is added to the reaction system to activate the nucleic acid amplification reaction, after the magnesium ion is added, the waveform occurs. Power supply, open ultrasonic 30s, so that the magnesium ions are fully mixed, then turn off the ultrasound, followed by ultrasound 30S, the frequency of 30s, the nucleic acid amplification reaction, the amplification condition room temperature 37 degrees Celsius, with blue flashlight and mobile phone The fluorescence intensity of different amplification durations, plotting fluorescence intensity and amplification time long relationship graph, the results of...

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to an ultrasonic integrated micro-droplet array detection platform and a preparation method and application thereof. The ultrasonic integrated micro-droplet array detection platform comprises a micro-column array, a waveform generator and a piezoelectric transducer, and the micro-column array comprises a substrate and a plurality of micro-columns extending out of one surface of the substrate; the piezoelectric transducer is in contact connection with the surface, deviating from the micro-column, of the substrate; and the waveform generator is connected with the piezoelectric transducer, and the piezoelectric transducer is used for converting an electric signal output by the waveform generator into ultrasonic waves. By utilizing the detection platform, the reaction of hundreds of or more to-be-detected samples can be simultaneously carried out, and the ultrasonic waves generated by the waveform generator are used for carrying out real-time on-demand non-contact stirring, mixing and dispersing on each liquid drop, so that the reaction rate is effectively improved, and high-flux, rapid and ultra-sensitive detection on the to-be-detected samples is realized.

Description

Technical field [0001] The present application belongs to biological detection technology, and particularly relates to an ultrasonic micro-droplet array detector integrated platform and its preparation and use. Background technique [0002] Nucleic acid detection is more suitable for the early stages of rapid and sensitive novel coronavirus infection diagnosis, prevention and control of pneumonia crown new pandemic. [0003] Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) due to its high sensitivity and specificity, it is considered the gold standard laboratory methods, and (SARS-CoV-2) viral nucleic acid detection crown new clinical diagnosis, however, RT-qPCR the method requires specialized expensive equipment, professional operation and long to get the results from the sample period of time, which for the less developed countries or resource-limited settings is often unrealistic, therefore, to develop a simple, rapid and of SARS-CoV -2 nucleic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/24C12M1/00C12Q1/70C12Q1/6844G01N1/38G01N21/64
CPCC12Q1/701C12Q1/6844G01N1/38G01N21/6428C12Q2531/119C12Q2537/1376C12Q2563/159C12Q2563/107Y02A50/30
Inventor 许太林周梦芸张学记
Owner SHENZHEN UNIV
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