Application of proline and alanine in prevention and treatment of wheat scab

A technology of wheat scab and alanine, which is applied in the field of metabolomics to achieve the effect of improving resistance

Active Publication Date: 2021-12-31
山东国仓健生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some fungicides in production can prevent and control wheat head blight to a certain extent, when the pathogens infect whea...

Method used

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  • Application of proline and alanine in prevention and treatment of wheat scab
  • Application of proline and alanine in prevention and treatment of wheat scab
  • Application of proline and alanine in prevention and treatment of wheat scab

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Phenotypic analysis of the disease-resistant variety Sumai 3 and the susceptible variety Shannong 20 infected with head blight

[0033] 1. Test method:

[0034] (1) Strain activation: The Fusarium graminearum strain used in this experiment was PH-1 strain. The strain was preserved in PDA medium at 4°C, and the strain was activated every three months. Cut one square centimeter of mycelium growth PDA medium with a scalpel and put it into CMC medium. Shake the bacteria at 200 rpm for three days at 28°C. After shaking the bacteria, filter the bacteria solution with a filter cloth, and pack in 50ml centrifuge tubes. Centrifuge at 2000rpm for 10min. Discard the supernatant, add 30ml sterilized water and shake to mix. Discard the supernatant, and resuspend the pellet with a small amount of sterile water. Detect the amount of bacteria under a microscope, and dilute to 1-5×10 according to the amount of spores 5 / ml concentration

[0035] (2) Inoculation method ...

Embodiment 2

[0038] Example 2: Analysis of differential metabolites based on metabolomics

[0039] 1. Non-targeted metabolomics analysis:

[0040] (1) Metabolite extraction method:

[0041] Take the spikelets of Sumai No. 3 and Shannong No. 20 after inoculation (Fusarium graminearum PH-1) 0, 3, 6, and 9 days respectively, grind the spikelet samples, and add the extract (methanol: acetonitrile : water, volume ratio 2:2:1), ultrasonication for 10min, centrifugation at 13000prm for 5min, take the supernatant and transfer it to a centrifuge tube, filter through a 0.22μm filter membrane before sample injection, and then load the sample.

[0042] (2) Liquid phase and mass spectrometry conditions of UPLC-QE-MS:

[0043] Liquid conditions: Chromatographic column: Thermo HYPERSIL GOLD C18 column (2.1×150, 1.7μm); column temperature: 35°C; mobile phase: A: 0.04% acetic acid solution, B: 0.04% acetonitrile; elution gradient: 0- 0.2min, A=90%; 0.2-6min, A decreases to 10%; 6-8min, A=10%; 8.1min, A ...

Embodiment 3

[0061] Example 3: Effects of External Application of Different Types of Amino Acids on Wheat Infection with Scab

[0062] 1. Test method:

[0063] Sensitive wheat Shannong 20 was used as the experimental material. The seeds were first placed at 4°C for 24 hours, then transferred to normal temperature for germination, and then cultivated under 16h light and 8h dark conditions. Take the second leaf of wheat at the three-leaf stage, cut it to 4 cm long, and dip both ends of the isolated leaf into 10 mM amino acids (glycine, methionine, arginine, proline, alanine, and cysteine) For 24 hours, water was used as a control treatment. Then, a wound was lightly poked in the middle of the adaxial surface of the leaf, and Fusarium graminearum PH-1 (3 μL) was inoculated on the wheat leaf. After 3 days, observe and take pictures to record the lesion area on the blade, and statistical data carry out significance analysis.

[0064] In order to further verify the impact of exogenous amino a...

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PUM

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Abstract

The invention discloses application of proline and alanine in prevention and treatment of wheat scab. The method comprises the following steps: firstly, based on a non-targeted metabonomics technology, screening resistance-related differential metabolites in a gibberellic disease-resistant wheat variety in an electric ion full scanning mode, and carrying out metabolic pathway enrichment analysis to find that an amino acid pathway is related to gibberellic disease resistance; and identifying proline and alanine in the amino acid pathway as fusarium head blight resistance related metabolites through a targeted metabonomics technology. According to the method, proline and alanine are treated before inoculation of in-vitro leaves and ears of wheat, so that the wheat can be effectively protected and treated, and the method is of great significance to prevention and treatment of wheat scab.

Description

technical field [0001] The invention relates to the technical field of metabolomics application, in particular to the application of proline and alanine in the prevention and treatment of wheat scab. Background technique [0002] Wheat scab is an epidemic disease of wheat caused by Fusarium graminearum complex. In recent years, due to changes in farming systems such as global warming, straw returning to the field, and the promotion and application of no-tillage techniques, and the country’s increasing demand for grain, the excessive planting density of wheat in production has caused changes in the microclimate in the field. Mildew presents an increasing trend year by year in our country, causing huge losses of wheat yield. Wheat head blight not only leads to serious reduction in grain yield and quality, but also mycotoxins produced by pathogenic bacteria in susceptible wheat kernels seriously threaten food safety and human and animal health. Therefore, the control of wheat...

Claims

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Application Information

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IPC IPC(8): A01N43/36A01N37/44A01P3/00
CPCA01N43/36A01N37/44
Inventor 胥倩王群青赵培迎谷淑波刘振
Owner 山东国仓健生物科技有限公司
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