Application of nicotinamide in preparation of medicine for resisting porphyromonas gingivalis

A technology of porphyromonas and gingival porphyrin, which is applied in the field of biomedicine, can solve the problems of subgingival microbial resistance, tetracycline superinfection and other problems, and achieve good biosafety performance, easy preparation, and good solubility Effect

Active Publication Date: 2022-01-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, minocycline, as a broad-spectrum antibacterial tetracycline antibiotic, may cause subgingival microorganisms to develop drug resistance, and there is also a risk of tetracycline teeth and superinfection

Method used

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  • Application of nicotinamide in preparation of medicine for resisting porphyromonas gingivalis
  • Application of nicotinamide in preparation of medicine for resisting porphyromonas gingivalis
  • Application of nicotinamide in preparation of medicine for resisting porphyromonas gingivalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Culture of strains and formulation of liquid inhibitors

[0034] 1 Preparation for testing strains:

[0035] In this example, the testing strain of the Toxaciopcellosis is obtained from the US Typical Microbial Square Savings Center, the strain number: ATCC BAA-308.

[0036] 2 Component and preparation of medium:

[0037] (1) Cow Hexin Tombsorbent (hereinafter referred to as BHI) Liquid medium Component and formulated method: 37 g of commercially available BHI powder is added to 1000 mL of distilled water, high temperature and high pressure (121.3 ° C, 103.4 kPa) steam sterilization 15 minutes, after cooling;

[0038] (2) Component of 5 μg / ml of Hemin solution and its formulation method: 40g of sodium hydroxide (NaOH) was added to 1000 mL of distilled water, formulated as a NaOH aqueous solution of 1 mol / L, will 50 mg Hemin powder is dissolved in the NaOH aqueous solution of 1 mol / L, and is set to 100 mL, high temperature and high pressure (121.3 ° C, 103.4...

Embodiment 2

[0047] Example 2: Effect of inhibitors on cell Porphyromonas gingivalis growth of bacteria

[0048] Aseptic procedure according to Example 1, the embodiment of Porphyromonas gingivalis Aeromonas bacterial suspension of Example 1 were inoculated in liquid media containing various concentrations of inhibitor to determine the state of growth inhibitors on Porphyromonas gingivalis Aeromonas Impact. Specific methods are as follows: a liquid medium containing various concentrations (8mg / mL, 4mg / mL, 2mg / mL) inhibitor, the culture in Example 1 embodiment to logarithmic phase (OD 600 ≈0.5) of Porphyromonas gingivalis Aeromonas bacterial suspension was diluted to OD 600 ≈0.1, at 37 ℃, strictly anaerobic (10% CO 2 , 10% H 2 , 80% N 2 ) Cultured under conditions for 24 hours, every two hours during reading the OD at a wavelength of 600nm, and the control group (without addition of a liquid medium inhibitor) is determined by comparing the growth of Porphyromonas gingivalis bacterium cell...

Embodiment 3

[0050] Example 3: inhibitor of Porphyromonas gingivalis Aeromonas hemagglutination Function of

[0051] Aseptic procedure according to Example 1, the embodiment of Porphyromonas gingivalis Aeromonas bacterial suspension of Example 1 were inoculated in liquid media containing various concentrations of inhibitor to determine an inhibitor of Porphyromonas gingivalis Aeromonas hemagglutination influence function. Specific methods are as follows: a liquid medium containing various concentrations (8mg / mL, 4mg / mL, 2mg / mL) inhibitor, the culture in Example 1 embodiment to logarithmic phase (OD 600 ≈0.5) of Porphyromonas gingivalis Aeromonas bacterial suspension was diluted to OD 600 ≈0.1, at 37 ℃, strictly anaerobic (10% CO 2 , 10% H 2 , 80% N 2 ) Cultured under conditions for 24 hours. 4 ℃, 5,000 × g rpm for 10 minutes. The precipitate was collected bacteria, table phosphate buffered saline (Phosphate Buffered Saline, referred to as PBS) of claim 1 bacteria resuspended precipitate ...

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Abstract

The invention discloses an application of nicotinamide in preparation of a medicine for resisting porphyromonas gingivalis. The nicotinamide disclosed by the invention is amide of nicotinic acid, is a clinical common medicine, is low in cost, wide in source and easy to prepare, and has good biological safety performance; and according to the application disclosed by the invention, the growth of the porphyromonas gingivalis, the erythrocyte agglutination capability and the activities of arginine-specific gingival protease and lysine-specific gingival protease of the nicotinamide serving as the inhibitor are measured, and experiments prove that the nicotinamide has an obvious inhibition effect on the periodontal disease toxicity caused by the porphyromonas gingivalis, is good in biological safety, tcan be applied to preparation of drugs for inhibiting porphyromonas gingivalis, especially to preparation of drugs for preventing and treating periodontitis, and can be used as a novel drug for preventing and treating periodontitis.

Description

Technical field [0001] The present invention belongs to the technical field of biomedicine, and more particularly to the application of nicotinamide in the preparation of antitrobacterial drugs. Background technique [0002] Periodontal disease occurs in pathological changes in teeth supporting tissue in the gum, a periodontal permeable, a toothcarcone and atribody. According to the new classification method proposed by the periodontal disease proposed at the International Seminar held by the American periodontal disease in 1999, periodontal disease can be divided into two major categories of gums and periodontitis. The early symptoms of periodontal disease often appear as gum bleeding and gums shape, color, and texture. As the condition is aggravated, there is no loss, the teeth can be formed, and the sulphonia absorbs, the severe person will have a looseness and even fall off. The oral health standards established by the World Health Organization (WHO) include the health requir...

Claims

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Application Information

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IPC IPC(8): A61K31/455A61P31/04A61P1/02
CPCA61K31/455A61P31/04A61P1/02
Inventor 李雨庆雷紫雪邱阳林永旺周学东
Owner SICHUAN UNIV
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