Multifunctional drug carrier material based on gold nanocage and preparation method thereof

A technology of gold nano-cages and carrier materials is applied in the fields of multifunctional drug carrier materials and their preparation, and the preparation of inorganic-organic hybrid drug carriers, which can solve the problem that drugs are difficult to penetrate into tumors, and achieve the effect of avoiding early release.

Active Publication Date: 2022-02-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]The current problem is how to further optimize the carrier material, further enhance its drug delivery ability, and allow the drug to penetrate more into the deep part of the tumor in pancreatic cancer to solve the problem of Existing drugs are difficult to penetrate into tumors

Method used

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  • Multifunctional drug carrier material based on gold nanocage and preparation method thereof
  • Multifunctional drug carrier material based on gold nanocage and preparation method thereof
  • Multifunctional drug carrier material based on gold nanocage and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0061] Example 1: Take SW1990 pancreatic cancer cell membrane / (gold nanocage-disulfide bond-gemcitabine / L-arginine) as an example.

[0062] 1) Synthesis of mercapto-polyethylene glycol-amino modified gold nanocages

[0063] Take 10 milliliters of ethylene glycol and heat it to 150 degrees Celsius in an oil bath under magnetic stirring, then add 0.12 milliliters of 3×10-3 mol / liter of sodium hydrosulfide in ethylene glycol) into the solution quickly. After 4 minutes, 1 mL of hydrogen chloride solution (prepared by adding 4 µl of 12 mol / L hydrochloric acid to 12 mL of ethylene glycol) was added to the mixture, followed by 2.5 mL of polyvinylpyrrolidone in ethylene glycol after 2 minutes. solution (prepared by dissolving 60 mg of polyvinylpyrrolidone in 3 ml of ethylene glycol). After another 2 minutes, 0.8 ml of 282×10-3 mol / L silver trifluoroacetate ethylene glycol solution was added to the mixed solution, and the reaction was continued for 1 hour at 150 degrees Celsius, washe...

example 1

[0074] The proton nuclear magnetic resonance spectrum of p-nitrophenyl-disulfide bond-gemcitabine in example 1 is as figure 1 As shown, the figure fully demonstrates the feasibility of the compound preparation.

[0075] The transmission electron micrograph of SW1990 pancreatic cancer cell membrane / (gold nanocage-disulfide bond-gemcitabine / L-arginine) in example 1 is as figure 2 Shown, fully proved the feasibility of the preparation method of the present invention.

[0076] The gemcitabine release pattern of SW1990 pancreatic cancer cell membrane / (gold nanocage-disulfide bond-gemcitabine / L-arginine) in example 1 under redox stimulation is as follows image 3 As shown, it fully proves that the gold nanocage drug carrier system of the present invention can release the drug gemcitabine under redox stimulation.

[0077] The nitric oxide release pattern of SW1990 pancreatic cancer cell membrane / (gold nanocage-disulfide bond-gemcitabine / L-arginine) under the action of active oxyge...

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Abstract

The invention discloses a multifunctional drug carrier material based on a gold nanocage and a preparation method thereof. The preparation method comprises the following steps: preparing the gold nanocage through an electrochemical replacement reaction between silver nanocubes and chloroauric acid, modifying sulfydryl-polyethylene glycol-amino to the surface of the gold cage by utilizing relatively strong interaction between the gold nanocage and a thiol group, modifying a gemcitabine prodrug responded by glutathione modified by p-nitrophenyl through a chemical reaction, and loading positively charged L-arginine through electrostatic interaction, and finally, wrapping by a tumor cell membrane through a liposome squeezer, so as to obtain the cell membrane-wrapped drug-loaded gold nanocage. The drug carrier disclosed by the invention has multifunctionality, namely multidrug delivery capacity, glutathione-responsive drug release performance, photo-thermal performance, tumor deep penetration effect and tumor targeting, and can be used for combined treatment of pancreatic malignant tumors.

Description

technical field [0001] The invention relates to a biomedical material, in particular to a gold nanocage-based multifunctional drug carrier material and a preparation method thereof, belonging to the technical field of preparation and application of inorganic-organic hybrid drug carriers. Background technique [0002] Pancreatic cancer is a malignant tumor with a very poor prognosis. Traditional chemotherapy drugs such as gemcitabine have very limited effects in the treatment of pancreatic cancer. On the one hand, the reason is that gemcitabine is rapidly metabolized and inactivated in the circulation. On the other hand, pancreatic cancer has a dense fibrotic matrix, which makes it difficult for small molecule drugs to penetrate, so the concentration of drugs in the tumor site is low. If in order to achieve the desired therapeutic effect, the dosage must be increased, which will cause damage to normal organs and tissues, and may also lead to drug resistance in the body. In o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/7068A61K31/198A61K47/69A61K47/60A61P35/00A61P1/18B82Y5/00B82Y40/00B22F9/24B22F1/054B22F1/06B22F1/102C07H1/00C07H19/067
CPCA61K41/0052A61K31/7068A61K31/198A61K47/6949A61K47/60A61P35/00A61P1/18B82Y5/00B82Y40/00B22F9/24C07H1/00C07H19/067A61K2300/00
Inventor 梁廷波张富李博文胡奇达
Owner ZHEJIANG UNIV
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