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Preparation method and application of bimetallic nano-enzyme

A bimetallic nano and metal organic framework technology, applied in the direction of heavy metal active ingredients, chemical instruments and methods, medical preparations of non-active ingredients, etc., can solve the problems of worsening Parkinson's disease, increased dopamine activity, etc., and achieve good catalytic performance , improve stability, choose reasonable effects

Pending Publication Date: 2022-04-15
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But decreased dopamine activity increases ROS production, which worsens Parkinson's disease

Method used

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  • Preparation method and application of bimetallic nano-enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Preparation and catalytic application of a kind of bimetallic nanozyme

[0034] (1) The hexagonal metal-organic framework ZIF-67 was prepared by solvent method.

[0035] Prepare to weigh 1.436g of cobalt nitrate hexahydrate and 3.244g of 2-methylimidazole in a 250mL Erlenmeyer flask, and then add 100mL of methanol to fully dissolve them. The two solutions were then mixed, stirred at room temperature for 13 min, and then left at room temperature for 24 h. After the reaction, the precipitate was recovered by centrifugation, washed with ethanol, and dried in a vacuum oven to obtain purple ZIF-67.

[0036] (2) Synthesis of nickel cobaltate NiCo by ion exchange method 2 O 4 .

[0037] Accurately weigh the NiCl of ZIF-67 and 150mg of the step (a) gained of 100mg 2 ·6H 2 O, place it in a 50mL reaction kettle, add 30mL of ethanol, and treat it under ultrasonic conditions for 30min. Subsequently, the reaction kettle was transferred to an electric blast oven and heated at ...

Embodiment 2

[0041] The material obtained in Example 1 is used to simulate the SOD enzyme activity, which will be mixed with riboflavin 20 μM, methionine 0.013M, NBT75 μM and different concentrations of NiCo 2 O 4 @PVP 0-100μg / mL PBS (pH 7.4, 100mM) buffer solution was irradiated for 30min under a constant light intensity UV lamp, and the absorbance of the mixed solution at 560nm was measured immediately after UV irradiation.

Embodiment 3

[0043] The material obtained in Example 1 was used to simulate oxidase and horseradish peroxidase activities, and 3 mL of sodium acetate buffer (1M, pH=4) and 200 μL of NiCo 2 O 4 @PVP (0.25mg / L) was mixed in the tube. Then, add 100 μL HO to the tube 2 O 2 and 200 μL TMB (5 mM, DMSO), after incubation for 2 minutes, the absorbance of the mixture was measured with a UV-Vis spectrophotometer.

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Abstract

The invention relates to a preparation method and application of a bimetallic nano enzyme, and the preparation method comprises the following steps: (a) preparing a hexagonal metal organic framework material ZIF-67 by adopting a solvent method; (b) synthesizing nickel cobaltate (NiCo2O4) by adopting an ion replacement method; and (c) preparing the NiCo2O4-coated PVP bimetallic nano-enzyme through a one-step template self-assembly method, so that the activity of superoxide dismutase (SOD enzyme) and the activity of catalase (CAT enzyme) can be well simulated, and. OH can be effectively removed. The preparation method disclosed by the invention has the beneficial effects that after macromolecular polyvinylpyrrolidone (PVP) is wrapped by the prepared bimetallic nano-enzyme NiCo2O4 with the oxygen vacancy, the stability of the nano-enzyme NiCo2O4 in simulated gastric juice and simulated intestinal juice is remarkably improved. The nano enzyme NiCo2O4-coated PVP can be used for well simulating the enzyme activity of SOD and the enzyme activity of CAT, and can be used for effectively eliminating. OH. The nano-enzyme NiCo2O4-coated PVP is electronegative under neutral and alkaline conditions, and the particle size of the nano-enzyme NiCo2O4-coated PVP is 1t; and the wavelength is 300 nm. The nano-enzyme NiCo2O4-coated PVP has no toxic or side effect, and can effectively eliminate the generation of active oxygen in cells.

Description

technical field [0001] The invention belongs to the research field of nanozymes, and relates to a preparation method and catalytic application of bimetallic nanozymes. Background technique [0002] Reactive oxygen species (ROS) mainly include superoxide anion, nitric oxide, hydrogen peroxide, hydroxyl radicals and singlet oxygen molecules. Low levels of ROS are necessary for the body, signal transduction, cell differentiation and apoptosis and other physiological processes require the participation of ROS, and excessive ROS will cause damage to the body through oxidation. The accumulation of ROS is a "time bomb" for the body, which can cause more serious diseases at any time. For example, excessive ROS can cause oxidative damage to DNA, proteins, lipids and other macromolecules, and can also induce the activation of caspases, leading to cell apoptosis. It has been reported that ROS is associated with at least 60 diseases. For example, in patients with Parkinson's disease,...

Claims

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Application Information

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IPC IPC(8): B01J23/755B01J33/00A61K9/00A61K9/14A61K33/24A61K47/32A61P1/00A61P35/00
Inventor 王硕刘敬民杨璐赵宁
Owner NANKAI UNIV
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