Preparation method and application of gold-copper bimetallic nano-enzyme composite material
A bimetallic nano-composite material technology is applied to the gold-copper bimetallic nano-enzyme composite material and its catalysis/photothermal antibacterial application field, which can solve the problems of low catalytic efficiency and weak interaction of gold-based nano-enzymes, and achieves The effect of promoting Fenton-like reaction, improving distribution density, and improving generation efficiency
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[0027] Example 1:
[0028] Preparation of lysozyme fiber;
[0029] Solution A was prepared by adding 0.015g glycine into 10mL hydrochloric acid solution with 1M concentration. Solution B was prepared by adding 0.1396g choline chloride into 1mL glacial acetic acid solution with a concentration of 1mM. 0.01g lysozyme was dissolved in 4750μLA solution and 250μL B solution. Stir and react in an oil bath at 70℃ for 5h. After the reaction, centrifuge at 120min00rpm and wash with ultrapure water twice, each time for 20 minutes. The final lysozyme fiber aqueous solution was 8ml.
[0030] Preparation of LNFs@Au / Cu Nanoenzyme Composite;
[0031] Take 800μL lysozyme fiber solution, add 800μL chloroauric acid and copper chloride solution, react for 30min, and add 800μL freshly prepared sodium borohydride solution, and quickly reduce to obtain metal nanoparticle composite.
[0032] Wherein the concentration of lysozyme fiber is 5mg / mL, the total concentration of metal salt solution is 0.15mM, ...
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[0035] Example 2:
[0036] In the same way as in Example 1, only changing the ratio of the gold-copper salt solution in the preparation step of the gold-copper nanoenzyme composite material, and performing LNFs@Au / Cu photothermal sterilization, the bacterial survival rate obtained is shown in Table 1. The results show that, compared with the unmodified gold nanoparticles, with the increase of copper content, the bactericidal efficiency of LNFs@Au / Cu increases, but with the further increase of copper content, the bactericidal efficiency decreases, which can be attributed to the increase of the size of LNFs@Au / Cu nanoparticles and the decrease of near infrared absorption, resulting in the decrease of photothermal conversion efficiency.
[0037] Table 1 Effect of different copper addition on photothermal sterilization of LNFs@Au / Cu
[0038] Addition amount of copper (molar ratio of Au to Cu) Bacterial survival rate (%) 1:0 65.13 4:1 28.24 3:1 27.5 2:1 56...
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[0039] Example 3:
[0040] In the same way as in Example 1, only changing the ratio of Au-Cu salt solution in the preparation step of Au-Cu nano-enzyme composite material, and carrying out catalytic hydrogen peroxide sterilization by LNFs@Au / Cu, the bacterial survival rate obtained is shown in Table 2. Therefore, with the increase of copper content, the catalytic sterilization efficiency of LNFs@Au / Cu increases, but with the further increase of copper content, the sterilization efficiency decreases, which can be attributed to the increase of size of LNFs@Au / Cu metal nanoparticles and the decrease of catalytic activity.
[0041] Table 2 Effect of different copper addition on catalytic sterilization of LNFs@Au / Cu
[0042] Addition amount of copper (molar ratio of Au to Cu) Bacterial survival rate (%) 1:0 87.26 4:1 68.13 3:1 61.34 2:1 73.46 1:1 91.74 0:1 100
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