Synthetic method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphoric acid

A technology of manganese-doped zinc sulfide and synthesis method, which is applied in chemical instruments and methods, alkali metal compounds, inorganic chemistry, etc., can solve the problems of time-consuming and labor-intensive preparation methods, complicated synthesis steps, etc., and achieves simplified solid-liquid separation process and synthesis. Simple process and safe, responsive effect

Pending Publication Date: 2022-05-17
常州磐诺仪器有限公司
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Problems solved by technology

However, the traditional material preparation method based on hydrophilic interaction liquid chromatography is time-consuming and laborious, the s

Method used

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  • Synthetic method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphoric acid
  • Synthetic method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphoric acid
  • Synthetic method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphoric acid

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Example Embodiment

[0044] Example 1

[0045] A method for synthesizing manganese-doped zinc sulfide quantum dots of glucose-6-phosphate, comprising the following steps:

[0046] S1: Dissolve 1.8 g of zinc sulfate heptahydrate and 0.1 g of manganese chloride tetrahydrate in 20 mL of deionized water; pour the obtained solution into a three-necked flask with a capacity of 250 mL, and stir under nitrogen protection for 30 min; take 1.5 g of sodium sulfide nonahydrate was dissolved in 5 mL of deionized water, added to a three-necked flask, and stirred for 30 min to obtain manganese-doped zinc sulfide quantum dots;

[0047] S2: Dissolve 500 mg of glucose-6-phosphate disodium in a mixed solution containing 75 mL of acetonitrile, 150 μL of trifluoroacetic acid and 50 mL of deionized water, add it to the three-necked flask of step S1, and stir at 25 °C for 6 h;

[0048] S3: fully washing the product obtained in step S2 with deionized water and ethanol, and drying in a vacuum dryer at 50° C. overnight to...

Example Embodiment

[0051] Example 2

[0052] Manganese-doped zinc sulfide quantum dots of glucose-6-phosphate for enrichment of glycopeptides in HRP:

[0053] (1) Preparation of sample: horseradish peroxidase (HRP) at 25mmol / LNH 4 HCO 3 Enzymatic hydrolysis in solution at 37°C for 16h;

[0054] (2) Enrichment: 0.5 mg of glucose-6-phosphate manganese-doped zinc sulfide quantum dots were dispersed into 10 μL of 1% by volume of trifluoroacetic acid, 9% by volume of acetonitrile and 90% by volume of deionized water. In the centrifuge tube, add 2 μL of the sample prepared in step (1), and enrich at 37°C for 30 min; fully wash with the buffer solution of 0.5% phosphoric acid, 14.5% deionized water and 85% acetonitrile by volume And centrifuged for 3 times; add 10 μL of 50% acetonitrile by volume and 50% by volume deionized water eluent, elute at 37°C for 30 min, and centrifuge to obtain the supernatant;

[0055] (3) Mass spectrometry analysis: Take 1 μL of the supernatant obtained in step (2) as a...

Example Embodiment

[0058] Example 3

[0059] Manganese-doped zinc sulfide quantum dots of glucose-6-phosphate for enrichment of glycopeptides in mixed proteins:

[0060] (1) Preparation of samples: bovine serum albumin (BSA) was first reductively alkylated with dithiothreitol and iodoacetamide, and then enzymatically hydrolyzed at 37 °C for 16 h; horseradish peroxidase (HRP) in 25 mM NH 4 HCO 3 Enzymatic hydrolysis at 37°C for 16h in the solution; bovine serum albumin (BSA) and horseradish peroxidase (HRP) were added to acetonitrile containing 1% by volume trifluoroacetic acid and 9% by volume at a molar ratio of 1000:1 and 90% volume ratio of deionized water in a centrifuge tube;

[0061] (2) Enrichment: 0.5 mg of the nanomaterial obtained in Example 1 was dispersed into 100 μL containing the glycopeptide of step (1) in 1% by volume of trifluoroacetic acid, 9% by volume of acetonitrile and 90% by volume of deionized In a centrifuge tube of water, enriched at 37°C for 30min; washed thoroughly...

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Abstract

The invention provides a synthesis method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphoric acid, carbohydrate D-glucose-6-sodium phosphate is combined with the manganese-doped zinc sulfide quantum dots by utilizing excellent biocompatibility and hydrophilicity of carbohydrate D-glucose-6-sodium phosphate and utilizing an affinity principle between phosphate groups and metal cations, so that the manganese-doped zinc sulfide quantum dots of the glucose-6-phosphoric acid are obtained. The manganese-doped zinc sulfide quantum dot of glucose-6-phosphoric acid, which is simple, convenient and safe in synthesis process, is prepared, and has ultrahigh specificity and selectivity on glycopeptide; magnetic ferroferric oxide is synthesized through a hydrothermal reaction, the surface of the magnetic ferroferric oxide is coated with a dopamine layer serving as a coupling connecting agent to be grafted with manganese-doped zinc sulfide quantum dots, the magnetic manganese-doped zinc sulfide quantum dots are obtained, the grafted manganese-doped zinc sulfide quantum dots serve as anchoring points to functionalize D-glucose-6-sodium phosphate, and the magnetic manganese-doped zinc sulfide quantum dots are obtained. According to the present invention, the hydrophilic property and the processability are improved, the strong responsiveness to the external magnetic field is provided, the solid-liquid separation process can be simplified, and the simultaneous separation and enrichment of glycopeptides and phosphopeptides can be achieved.

Description

technical field [0001] The invention relates to the field of functionalized nanomaterials and nanotechnology, in particular to a synthesis method and application of manganese-doped zinc sulfide quantum dots of glucose-6-phosphate. Background technique [0002] Diabetes is a metabolic disease characterized by hyperglycemia, and the biomarkers of diabetes are diverse. Among them, autoantibodies have been confirmed, but the timely diagnosis ability for the inherent heterogeneity of diabetes needs to be improved urgently. In the pathogenesis of diabetes, protein glycosylation plays a key role. As a kind of protein modification, glycosylation widely exists in human proteins. Protein glycosylation has pervasive effects on cellular processes such as intercellular signal transduction, immune regulation, and cell differentiation. Studies have shown that abnormal protein glycosylation is related to physiological abnormalities and pathology, and some glycosylated proteins have become...

Claims

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Application Information

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IPC IPC(8): B01J20/281B01J20/28B01J20/22B01J20/30G01N30/08
CPCB01J20/281B01J20/28009B01J20/22B01J20/0285G01N30/08Y02E60/10
Inventor 王涵文谢泽虎
Owner 常州磐诺仪器有限公司
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