Fibroneclastin composition freeze-dried micro-core with skin barrier repairing effect and preparation method thereof

A skin barrier repair and elastin technology, which is applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of easy absorption and reduced activity loss

Pending Publication Date: 2022-05-31
WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, biological skin care products focus on biologically active proteins, pay attention to the maintenance of biological activity of the active ingredients of the product, do not add preservatives, do not add artificial chemical ingredients, are not irritating to the skin, can quickly repair the skin barrier, and have better skin repair effects than Traditional cosmetics, but there is no equivalent product for sensitive skin repair

Method used

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  • Fibroneclastin composition freeze-dried micro-core with skin barrier repairing effect and preparation method thereof
  • Fibroneclastin composition freeze-dried micro-core with skin barrier repairing effect and preparation method thereof
  • Fibroneclastin composition freeze-dried micro-core with skin barrier repairing effect and preparation method thereof

Examples

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preparation example Construction

[0022] The preparation method of the fibronectin involved in the present invention is as follows:

[0023] (1) Obtain the fibronectin (FN) gene through GenBank query, insert the FN gene sequence into pMD19-TSimple Vector, its 5' end is connected with the NotⅠ restriction site (GCGGCCGC), and the 3' end is connected with the XbaⅠ restriction site (TCTAGA), thus obtaining the plasmid pMD19-T-FN;

[0024] (2) Use endonuclease Not I and endonuclease Xba I to perform double enzyme digestion on plasmid pYES2 / CT-MFα and plasmid pMD19-T-FN, respectively. The enzyme digestion reaction system is:

[0025] Double Enzyme Digestion System Volume (μL) Quick Cut Not I 2.0 QuickCut Xba I 2.0 10xQuickCut Green Buffer 5 pYES2 / CT-MFa or FN fragment 15 wxya 2 o

26 Total Volume 50

[0026] Enzyme digestion reaction was carried out at 37°C for 3 hours in a metal bath, and the digestion products were detected by 2% agarose gel electrophoresis, ...

Embodiment 1

[0042] A fibronectin elastin composition freeze-dried microcore with skin barrier repairing effect, which is made of the following raw materials in weight percentage: 15ppm fibronectin, 15ppm platelet-derived growth factor, 15ppm metallothionein type IV, elastin Protein 15ppm, filaggrin 20ppm, mannitol 6%, glycine 6%, trehalose 1%, and the balance is water.

[0043] The preparation method of described freeze-dried microcore comprises the following steps:

[0044] (1) mixing each raw material of formula quantity to obtain mixed solution;

[0045] (2) Using a high-precision fluid dispensing system, the mixed solution is dropped into liquid nitrogen stored in a Dewar bottle drop by drop at a rate of 10 μl to freeze into microcores;

[0046] (3) Take out the microchips and put them into vials, put 10 frozen microchips into each vial, and freeze-dry them. The program control of the freeze-drying is shown in Table 1.

[0047] Table 1 freeze-drying program parameter table

[0048]...

Embodiment 2

[0051] A fibronectin elastin composition freeze-dried microcore with skin barrier repairing effect, which is prepared from the following raw materials in weight percentage: 10 ppm of fibronectin, 10 ppm of platelet-derived growth factor, 10 ppm of metallothionein type IV, elastin Protein 10ppm, filaggrin 40ppm, mannitol 6%, glycine 6%, trehalose 1%, and the balance is water.

[0052] The preparation method of the freeze-dried microcore is the same as in Example 1.

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Abstract

The invention discloses a fibronectin composition freeze-dried micro-core with a skin barrier repairing effect and a preparation method thereof. The freeze-dried micro-core is prepared from the following raw materials in percentage by weight: 10 to 15 ppm of fibronectin, 10 to 15 ppm of platelet-derived growth factors, 10 to 15 ppm of metallothionein type IV, 10 to 15 ppm of elastin, 20 to 40 ppm of fibroin, freeze-drying protection liquid and the balance of water. The pharmaceutical composition is a biological functional product, has cell proliferation promoting activity and cell migration promoting activity, shows the effect of relieving stabbing pain in lactic acid stabbing pain experiments, is free of preservatives and artificial chemical components during preparation, and is non-irritant to skin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fibronectin elastin composition freeze-dried microcore with skin barrier repairing effect and a preparation method thereof. Background technique [0002] Sensitive skin is a state of highly sensitive skin. In this state, the skin is easily affected by external factors, and discomfort such as skin itching, tightness, burning, and stinging occurs. Studies have shown that sensitive skin is increasing worldwide, and the incidence rate of sensitive skin among Asian women is 40% to 55%, among which the incidence rate of Chinese women is 36.1%. Sensitive skin, as a skin problem with a high incidence in modern society, has seriously affected people's study, work and life. There are a lot of skin care products on the market that claim to have the effect of repairing sensitive skin, and there are many products with poor efficacy or even harmful to the skin. [0003] With the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/02A61K8/34A61K8/60A61Q19/00A61Q19/08
CPCA61K8/64A61K8/60A61K8/345A61K8/0241A61Q19/005A61Q19/08A61Q19/004A61K2800/30A61K2800/5922A61K2800/84
Inventor 赵俊朱何龙刘家炉李媛媛周炜夏兵兵何志远许高涛王梦
Owner WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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