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Method for extracting corilagin from emblic leafflower fruit and application of corilagin

A technology of emblica and emblica fruit, applied in the fields of application, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of potato tuber reduction, yield reduction, side effects, etc., and achieve simple and easy operation in the preparation process, avoid The effect of waste and easy availability of raw materials

Pending Publication Date: 2022-06-07
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the use of chemical agents to control the disease is effective, it also has some side effects, such as reducing potato tubers, reducing yields, and posing threats to the environment and human health

Method used

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  • Method for extracting corilagin from emblic leafflower fruit and application of corilagin
  • Method for extracting corilagin from emblic leafflower fruit and application of corilagin
  • Method for extracting corilagin from emblic leafflower fruit and application of corilagin

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Effect test

Embodiment 1

[0031] A method for preparing corylragin from emblica seeds, comprising the following steps:

[0032] Step 1: Take 740g of fresh emblica fruit, denuclearize and juice, and get 234g of pomace. The emblica pomace was placed in a 5000 mL beaker, soaked in 2000 mL 70% ethanol and accelerated with ultrasound for 1 h, repeated dip 3 times, combined with the extract solution, and the solvent was drained by rotating the evaporator to give the ethanol layer crude extract (EA) 19.74g.

[0033] Step 2, take the ethanol layer crude extract (EA) obtained in step 1 19.74g, dissolve with 50mL 50% methanol water, and use medium and low pressure reversed phase C18 column (filler: YMC-GEL ODS-A-HG, s-50μm; Column length is 4.5×35 cm), flow rate is set to 30 mL / min, elution with 25% aqueous methanol solution, and the 31st to 36min components (EA-2) are collected.

[0034] Step 3, take step 2 (EA-2) 1.83g dissolved with 15mL 100% methanol, with Sepphadex LH-20 (2.5×170cm), 100% methanol as eluent, ov...

Embodiment 2

[0050] In the ultra-clean workbench, take the strains preserved in the inclined medium (Streptomyces potato scabs AMCC400023), carefully pick up the single colonies by the burning cooling inoculation ring next to the alcohol lamp flame, use the method of plate scribing, inoculate the pathogenic bacteria in Gao's No. 1 solid medium, seal the edge of the membrane, and let it stand in a constant temperature biochemical incubator at 30 °C for 48 h. Among them, Gord's No. 1 solid medium (g / L): potassium nitrate: 1.0; potassium dihydrogen phosphate: 0.5; magnesium sulfate: 0.5; ferrous sulfate: 0.01; sodium chloride: 0.5; soluble starch: 20.0; agar: 15.0.

[0051] Use the inoculation ring to pick up the activated single colony of Streptomyces scabbedus in potatoes, and closely stroke on The Gao's No. 1 medium, and let it stand at 28 °C for 3 days; Aspirate 3 mL of sterile water on the plate, gently scrape off the surface spores with a blade, rinse the surface spores of the medium in sma...

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Abstract

The invention discloses a method for extracting corilagin from phyllanthus emblica and application of the corilagin, and the method comprises the following steps: extracting phyllanthus emblica pomace with ethanol to obtain an ethanol layer crude extract; dissolving the ethanol layer crude extract with methanol water, separating and purifying with a reversed-phase C18 column, and collecting the 31-36th min component as a component EA-2; dissolving the component EA-2 by using methanol, separating and purifying by using Sephadex LH-20, and collecting the component from 3180 to 4620 minutes to obtain the component EA-2-2; and dissolving the component EA-2-2 with methanol, purifying the dissolved component EA-2-2 with a high performance liquid chromatograph, and collecting the 14-16 min component as the component EA-2-2-1. Therefore, the phyllanthus emblica pomace can be fully recycled, waste is avoided, the obtained component is EA-2-2-1 corilagin, and the compound can efficiently antagonize potato streptomyces scabies and has important application value in prevention and treatment of potato scab.

Description

Technical field [0001] The present invention relates to the field of active pharmaceutical technology, specifically to a method of extracting corillagin from emblica seeds and application thereof. Background [0002] Emblica L. (phyllanthus emblica L.) It is the fruit of the genus Phyllanthus in the euphoraceae family Euphoraceae. Mainly distributed in the tropics, subtropics; Native to India, distributed in Pakistan, Malaysia, China and other countries; Fujian and Yunnan provinces have the largest production in China. As early as 1998, it was included in the list of "both food and medicine" by the Ministry of Health, included in several editions of the Pharmacopoeia of the People's Republic of China, and designated by the United Nations Health Organization as one of the three health plants promoted and cultivated worldwide. The special composition and pharmacological activity of emblica seeds make it have good development and application value, and related industries have also d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H13/08A01N43/16A01P1/00
CPCC07H1/08C07H13/08A01N43/16Y02A50/30
Inventor 李健吴秀娜张铃玉吴达仁
Owner JIMEI UNIV
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