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Glycopeptide-enriched polymer material as well as preparation and application thereof

A technology of polymer materials and polymerization reactions, which is applied in the fields of material analysis chemistry and organic chemistry, can solve the problems of inability to enrich glycopeptides, non-specific adsorption of acidic peptide chains with basic residues of glycopeptides that are difficult to retain, and glycopeptides that are difficult to wash. To achieve the effect of controllable polymer layer, high repeatability and enrichment, and easy repeatability

Pending Publication Date: 2022-06-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have the problems of difficult elution of glycopeptides, non-specific adsorption of acidic peptide chains, or difficulty in retention of glycopeptides with basic residues, so the purpose of large-scale enrichment of glycopeptides in complex biological samples cannot be achieved.

Method used

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  • Glycopeptide-enriched polymer material as well as preparation and application thereof
  • Glycopeptide-enriched polymer material as well as preparation and application thereof
  • Glycopeptide-enriched polymer material as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Preparation of glycopeptide enriched materials

[0044] The dipeptide functional monomer structure composed of tryptophan and arginine is as follows figure 1 shown. Into a 50ml three-necked flask were sequentially added 0.5mmol tryptophan-arginine dipeptide monomer, 0.005mmol chain transfer reagent 4-cyanovaleric acid dithiobenzoic acid, 0.001mmol initiator azobisisobutyramidine hydrochloride, the molar ratio is 500:5:1, and then 0.5 g of mercapto silica gel (particle size 5 μm, pore size is added) ), while adding 6 mL of ultrapure water and 6 mL of methanol as solvent. Nitrogen was introduced under stirring, and after thorough mixing, the reaction system was evacuated and filled with nitrogen to remove residual oxygen in the reaction system; the temperature of the flask was controlled at 70 ° C and left to react for 48 hours; Ionized water (H 2 O) Sequentially washing the polymer grafted surface to obtain glycopeptide-enriched material, and drying in an oven.

[...

Embodiment 2

[0047] The dipeptide monomer copolymer composed of arginine and tryptophan was grafted onto the surface of porous silica gel as described in Example 1, and an infrared characterization experiment was carried out. image 3 The infrared absorption spectrum of the copolymer modified material is shown, indicating that the dipeptide monomer has been successfully bonded to the surface of the porous silica gel. Of which, ~1640cm -1 The wave peak at is attributed to the absorption peak of C=O; ~2900cm -1 The peaks at are assigned to the methyl peak and the methylene peak.

Embodiment 3

[0049] As described in Example 1, the dipeptide monomer composed of arginine and tryptophan and the sialic acid monosaccharide molecule were subjected to isothermal titration experiments. Figure 4 It is shown that there is an interaction between the dipeptide molecule and the sialic acid monosaccharide molecule, and the two generate exothermic heat after binding, and the binding is carried out in a 1:2 binding manner, and the relevant binding coefficient K is shown in the figure.

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Abstract

The invention relates to the fields of material science, analytical chemistry and organic chemistry. The invention provides a preparation method and application of a polymer material for glycopeptide enrichment. The glycopeptide enrichment material comprises a matrix and a copolymer layer formed on the surface of the matrix. According to the method, a reversible addition-fragmentation chain transfer free radical polymerization reaction mechanism is utilized, and a polymer chain with a glycopeptide enrichment function is grafted on the surface of a matrix. The material is organically combined with a column solid-phase extraction mode or a dispersive solid-phase extraction mode, so that high-selectivity, high-repeatability and high-flux enrichment of glycopeptides in a complex biological sample can be realized, and the identification number of glycoproteins can be remarkably increased. Therefore, the method is expected to be widely applied to the aspects of glycopeptide enrichment, glycoprotein identification and the like.

Description

technical field [0001] The invention relates to the fields of material analysis chemistry and organic chemistry, in particular to a glycopeptide enrichment material and a preparation method and application thereof. Background technique [0002] Protein glycosylation is one of the most common and important post-translational modifications of proteins in organisms, so glycoproteomics research has attracted extensive attention of many biological scientists. However, due to the very low absolute content of glycoproteins in organisms, the current technical strategy for glycoproteomics research is mainly after enrichment at the protein or peptide level. The existing glycopeptide enrichment methods mainly include: metal oxide method, hydrazine chemical method, boronic acid affinity method and the combination of the above-mentioned techniques. However, these methods have the problems of difficulty in elution of glycopeptides, non-specific adsorption of acidic peptide chains, or dif...

Claims

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Application Information

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IPC IPC(8): C08F292/00C08F8/30C08F120/60G01N1/40G01N27/62C07K1/14
CPCC08F292/00C08F8/30G01N27/62G01N1/405C07K1/14C08F220/603C08F120/60
Inventor 梁鑫淼李秀玲董雪芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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