Glycopeptide-enriched polymer material as well as preparation and application thereof

A technology of polymer materials and polymerization reactions, which is applied in the fields of material analysis chemistry and organic chemistry, can solve the problems of inability to enrich glycopeptides, non-specific adsorption of acidic peptide chains with basic residues of glycopeptides that are difficult to retain, and glycopeptides that are difficult to wash. To achieve the effect of controllable polymer layer, high repeatability and enrichment, and easy repeatability

Pending Publication Date: 2022-06-21

DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

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AI-Extracted Technical Summary

Problems solved by technology

However, these methods have the problems of difficult elution of glycopeptides, non-specific adsorption of acidic peptide chains, or difficulty in rete...

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Method used

In summary, the glycopeptide enrichment material of the present invention has good selective enrichment performance for glycopeptides, and compared with conventional metal oxides, the enrichment of glycopeptides by polymer modified silica gel materials has higher selective. Utilizing the high-efficiency and specific adsorption capacity ...

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Abstract

The invention relates to the fields of material science, analytical chemistry and organic chemistry. The invention provides a preparation method and application of a polymer material for glycopeptide enrichment. The glycopeptide enrichment material comprises a matrix and a copolymer layer formed on the surface of the matrix. According to the method, a reversible addition-fragmentation chain transfer free radical polymerization reaction mechanism is utilized, and a polymer chain with a glycopeptide enrichment function is grafted on the surface of a matrix. The material is organically combined with a column solid-phase extraction mode or a dispersive solid-phase extraction mode, so that high-selectivity, high-repeatability and high-flux enrichment of glycopeptides in a complex biological sample can be realized, and the identification number of glycoproteins can be remarkably increased. Therefore, the method is expected to be widely applied to the aspects of glycopeptide enrichment, glycoprotein identification and the like.

Application Domain

Preparing sample for investigationMaterial analysis by electric/magnetic means +1

Technology Topic

GlycoproteinPolymer chemistry +7

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Examples

Experimental program(9)

Example Embodiment

[0042] Example 1

[0043] Preparation of glycopeptide enriched materials

[0044] The dipeptide functional monomer structure composed of tryptophan and arginine is as follows figure 1 shown. Into a 50ml three-necked flask were sequentially added 0.5mmol tryptophan-arginine dipeptide monomer, 0.005mmol chain transfer reagent 4-cyanovaleric acid dithiobenzoic acid, 0.001mmol initiator azobisisobutyramidine hydrochloride, the molar ratio is 500:5:1, and then 0.5 g of mercapto silica gel (particle size 5 μm, pore size is added) ), while adding 6 mL of ultrapure water and 6 mL of methanol as solvent. Nitrogen was introduced under stirring, and after thorough mixing, the reaction system was evacuated and filled with nitrogen to remove residual oxygen in the reaction system; the temperature of the flask was controlled at 70 ° C and left to react for 48 hours; Ionized water (H 2 O) Sequentially washing the polymer grafted surface to obtain glycopeptide-enriched material, and drying in an oven.

[0045] In the present invention, the material of the matrix is Si, Cu, Ag, Au, Pt, CuO, Fe 3 O 4 , Porous SiO 2 , Porous Al2 O 3 , Porous TiO 2 , Porous ZrO 2 , Sepharose or Sepharose, or a mixture of two or more in any ratio. In this embodiment, the selected matrix material is thiol-modified silica gel. figure 2 Schematic diagram for the synthesis of the copolymer-grafted silica gel samples.

Example Embodiment

[0046] Example 2

[0047] The dipeptide monomer copolymer composed of arginine and tryptophan was grafted onto the surface of porous silica gel as described in Example 1, and an infrared characterization experiment was carried out. image 3 The infrared absorption spectrum of the copolymer modified material is shown, indicating that the dipeptide monomer has been successfully bonded to the surface of the porous silica gel. Of which, ~1640cm -1 The wave peak at is attributed to the absorption peak of C=O; ~2900cm -1 The peaks at are assigned to the methyl peak and the methylene peak.

Example Embodiment

[0048] Example 3

[0049] As described in Example 1, the dipeptide monomer composed of arginine and tryptophan and the sialic acid monosaccharide molecule were subjected to isothermal titration experiments. Figure 4 It is shown that there is an interaction between the dipeptide molecule and the sialic acid monosaccharide molecule, and the two generate exothermic heat after binding, and the binding is carried out in a 1:2 binding manner, and the relevant binding coefficient K is shown in the figure.

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Description & Claims & Application Information

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Easy to repeat
Easy to operate

Glycopeptide-enriched polymer material as well as preparation and application thereof

AI-Extracted Technical Summary

Problems solved by technology

Method used

Abstract

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Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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