Glycopeptide-enriched polymer material as well as preparation and application thereof
A technology of polymer materials and polymerization reactions, which is applied in the fields of material analysis chemistry and organic chemistry, can solve the problems of inability to enrich glycopeptides, non-specific adsorption of acidic peptide chains with basic residues of glycopeptides that are difficult to retain, and glycopeptides that are difficult to wash. To achieve the effect of controllable polymer layer, high repeatability and enrichment, and easy repeatability
Pending Publication Date: 2022-06-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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The invention relates to the fields of material science, analytical chemistry and organic chemistry. The invention provides a preparation method and application of a polymer material for glycopeptide enrichment. The glycopeptide enrichment material comprises a matrix and a copolymer layer formed on the surface of the matrix. According to the method, a reversible addition-fragmentation chain transfer free radical polymerization reaction mechanism is utilized, and a polymer chain with a glycopeptide enrichment function is grafted on the surface of a matrix. The material is organically combined with a column solid-phase extraction mode or a dispersive solid-phase extraction mode, so that high-selectivity, high-repeatability and high-flux enrichment of glycopeptides in a complex biological sample can be realized, and the identification number of glycoproteins can be remarkably increased. Therefore, the method is expected to be widely applied to the aspects of glycopeptide enrichment, glycoprotein identification and the like.
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