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Method for preparing Fuzhuan tea polysaccharide with hypoglycemic and lipid-lowering functions

A technology for Fu brick tea and tea polysaccharide, applied in the field of preparation of Fu brick tea tea polysaccharide, can solve problems such as difficult problems, structure and composition complexity, and achieve the effects of accelerating dissolution rate, good elution separation effect, and short extraction time.

Pending Publication Date: 2022-07-22
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the structure and composition of the enzymatically hydrolyzed materials, it is still a technical problem to prepare Fuzhuan brick tea polysaccharides with the functions of lowering blood sugar and fat.

Method used

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  • Method for preparing Fuzhuan tea polysaccharide with hypoglycemic and lipid-lowering functions
  • Method for preparing Fuzhuan tea polysaccharide with hypoglycemic and lipid-lowering functions
  • Method for preparing Fuzhuan tea polysaccharide with hypoglycemic and lipid-lowering functions

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preparation example Construction

[0029] (1) Preparation technology of Fuzhuan tea polysaccharide

[0030] Aiming at the problems that the existing extraction technology is difficult to fully extract the polysaccharides in Fu brick tea, wastes raw materials, and at the same time the extraction purity of Fu brick tea polysaccharides is not high, the present invention proposes a method based on Fu brick tea enzymatic hydrolysis extraction, fiber column chromatography separation The preparation method of Fuzhuan tea tea polysaccharide.

[0031] 1.1 The source and origin of Fu brick tea

[0032] Fu brick tea comes from the plant dark tea, which is produced in Shaanxi.

[0033] 1.2 Preparation of acetic acid-acetate buffer solution

[0034] Acetic acid-sodium acetate buffer solution (pH 5.0-6.0): take 54.6 g of sodium acetate, add 20 mL of 1 mol / L acetic acid solution to dissolve, and then add water to dilute to 500 mL.

[0035] The pH required for the experiment was adjusted by adding 0.1 mol / L acetic acid and ...

preparation example

[0046] according to figure 1 , select the technological parameters with higher yield of tea crude sugar to complete the enzymatic extraction of Fuzhuan tea, and conduct the salt washing experiment of polysaccharide with different concentrations of NaCl solution in the separation of fiber column chromatography.

example 1

[0048] (1) after the Fu brick tea is pulverized, cross 80 mesh sieves to obtain Fu brick tea tea powder;

[0049](2) After pulverizing the Fuzhuan tea, add 30 mL of deionized water to 1 g of Fuzhuan tea powder according to the material-to-liquid ratio, and then add 4 mL of acetic acid-vinegar containing compound enzymes (the mass ratio of cellulase:papain is 3:1) salt buffer solution, put the mixed enzymatic hydrolysis system (complex enzyme concentration of 2%, mass fraction) in a water bath, and enzymatic hydrolysis for 60 min under the conditions of enzymatic hydrolysis pH 5 and enzymatic hydrolysis temperature of 55 °C, enzymatic hydrolysis After completion, the enzyme was inactivated in a boiling water bath at high temperature for 15min to obtain an extract, which was filtered under reduced pressure with a vacuum filtration device to remove insolubles in the extract, and then concentrated under reduced pressure using a rotary evaporator (60°C water temperature condition) ...

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Abstract

The invention discloses a method for preparing Fuzhuan tea polysaccharide with functions of reducing blood sugar and blood fat. The method comprises the following steps: crushing Fuzhuan tea, adding deionized water according to a solid-liquid ratio of 1: 30g / mL, then adding a compound enzyme with an enzyme concentration of 2% and an acetic acid-acetate buffer solution, carrying out heating enzymolysis for 60 min in a water bath kettle with a temperature of 55 DEG C, filtering an enzymolysis extraction liquid, carrying out concentration, deproteinization and alcohol precipitation, redissolving a precipitate with deionized water, carrying out freeze drying, dissolving a freeze-dried sample, separating the sample in a DEAE-52 cellulose column, and carrying out freeze-drying to obtain the Fuzhuan tea. And dialyzing, concentrating and freeze-drying the eluent to obtain the Fuzhuan tea polysaccharide. The Fuzhuan tea polysaccharide prepared by the method has the advantages of high yield, high purity, short extraction time, energy consumption reduction, mild process conditions, capability of effectively preserving the biological activity of the polysaccharide and the like, and has good effects of reducing blood sugar and blood fat.

Description

technical field [0001] The invention relates to an extraction technology of active tea polysaccharide, in particular to a preparation method of Fuzhuan tea polysaccharide. Background technique [0002] Fu brick tea is a kind of dark tea made from black hair tea as raw material, through steaming, stacking, pressing, forming, and blooming. Fuzhuan tea belongs to post-fermentation tea. It grows and reproduces with tea as a medium to produce golden yellow closed capsules, forming golden yellow particles visible to the naked eye inside the tea brick, commonly known as "Golden Flower Fungus", and at the same time transforms tea leaves through the metabolic activity of "Golden Flower Fungus" The various components of the tea form a unique color, fragrance and quality characteristics. The tea soup is orange-red or yellow, with a mellow and sweet taste, and a strong "fungus flower fragrance". Long-term drinking of Fu brick tea can help digestion, effectively promote human metabolism...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/82A61P3/06A61P3/10A23L33/125
CPCC08B37/0003A61K31/715A61K36/82A61P3/10A61P3/06A23L33/125A23V2002/00A23V2200/3262A23V2200/328A23V2250/51Y02A50/30
Inventor 杨文娟龚频姚文博刘萌王静李楠
Owner SHAANXI UNIV OF SCI & TECH
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