This invention provides a
microfluidic chip, including a substrate (1), on which sample loading holes (2) and a plurality of detection holes (6) are provided. A multi-level microchannel is connected between the sample loading holes (2) and the detection holes (6). The sample loading holes (2) are located in the
central region of the substrate (1) and are used to introduce samples. The detection holes (6) are uniformly distributed on the outer periphery of the sample loading holes (2) and are used to detect samples. The multi-level microchannel is characterized by its
radial distribution starting from the sample loading holes (2) and connecting the sample loading holes (2) and the detection holes (6) through hierarchical flow channels, thereby sequentially guiding the samples from the sample loading holes (2) to the detection holes (6). This design better meets the needs of complex sample analysis and efficient detection.