The invention relates to a high-temperature and high-pressure visual microscopic oil reservoir chip holder and a use method thereof, wherein the holder is characterized in that the chip holder comprises a holder main body; the holder main body comprises an annular base, an annular top cover, a sapphire lens arranged at the lower part of the base, a press plate for fixing the sapphire lens, anothersapphire lens arranged on the top of the top cover, another press plate for fixing the another sapphire lens, and a microfluidic chip arranged on the base; the base is in blot connection with the topcover, and the microfluidic chip is fixed by an extrusion force between the base and the top cover; a space enclosed by the top cover, the second sapphire lens and the microfluidic chip is an upper seal cavity body, the upper seal cavity body is connected with an upper cavity body connector, and the upper cavity body connector communicates with the outside; a space enclosed by the base, the firstsapphire lens and the microfluidic chip is a lower seal cavity body, the lower seal cavity body is connected with a lower cavity body connector, and the lower cavity body connector communicates withthe outside. The holder can be widely applied in the field of micro-scale effect research.