The invention relates to a production method of a low cholesterol, iron-rich and magnesium-rich egg. The method comprises the steps of: preparing a Chinese herbal composition comprising Schisandra chinensis, Salvia Miltiorrhiza, licorice, radix paeoniae rubra, Radix Trichosanthis, bighead atractylodes rhizome, Ligusticum wallichii, and astragalus; preparing a nutritional composition composed of lactic acid bacteria, methionine, zinc sulfate, magnesium carbonate, glucose, vitamin B6, vitamin K, vitamin C, fat, Tenebrio molitor powder, hawthorn powder and amino acid chelated iron; according to a weight proportion, taking 50 parts of laying hen's complete feed and 0.5-1 part of the Chinese herbal composition to prepare feed A, and feeding a 60-day-old laying hen continuously with the feed A until the laying hen is 125 days old; and according to a weight proportion, taking 50 parts of laying hen's complete feed and 0.5-1 part of the nutritional composition to prepare feed B, and feeding a laying hen of over 125 days old with the feed B continuously in a whole egg producing period. Eating of the egg provided by the invention can supplement magnesium, iron and other trace elements at the same time.