The application discloses a three-dimensional activity track tracking and measuring and recording method of animals based on a depth camera, and mainly comprises the following steps: S1, installing the depth camera at the front side of a transparent cage at a distance of 1.5-2 m, adjusting the distance according to the size of the cage, and making the
field of view cover the front of the cage and the picture as large as possible; S2, accurately positioning eight vertexes in the cage; S3, acquiring and removing the
background noise points in real time; S4, simultaneously measuring and recording the three-dimensional track data in real time, calling the
infrared camera of Kinect2.0, synchronously recording the four-part picture, and facilitating the experimenter to review the data; and the method is suitable for measuring the activity amount, movement,
muscle tension and other related behavior indexes of experimental animals having three-dimensional activities in the cage, such as accurate activity amount, bounce frequency, position preference, movement track, whether there is an activity mode, track
abnormality and the like, and the measurement of the indexes is helpful to the modeling of various senile degenerative diseases, the effect evaluation of
drug effect and the effect evaluation of
rehabilitation therapy.