Electrochemical supermicro electrode preparing process

A preparation process and electrochemical technology, which is applied in the field of biosensors and electroanalytical chemistry, can solve the problems that composite microelectrodes cannot adapt, and achieve the effects of easy control of production conditions, reduced disturbance, and good stability

Inactive Publication Date: 2009-06-24
SAMXON ELECTRONICS DONG GUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned method adopts a three-level electrode system, and the composite microelectrode obtained according to this method is only provided with a relatively stable reference potential by a micro-reference electrode in cell measurement, although the measurement accuracy is improved to a certain extent. degree, but the prepared composite microelectrodes cannot adapt to and meet the requirements of neurotransmitters, nitric oxide (NO), hydrogen peroxide (H 2 o 2 ) and oxygen free radicals and other information molecules for in vivo, real-time and dynamic chemical detection requirements

Method used

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  • Electrochemical supermicro electrode preparing process
  • Electrochemical supermicro electrode preparing process
  • Electrochemical supermicro electrode preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: carbon fiber / gold (CF / Au) ultrafine composite electrode

[0050] In this embodiment, carbon fiber electrodes and gold are selected as the combination of the two electrodes, and ethanol and double distilled water are used for ultrasonic cleaning; the conductive adhesive is silver conductive adhesive; the insulating layer is formed by polymerization. The specific manufacturing process is as follows: first place the carbon fiber electrode in acetone to reflux for 8 hours, then ultrasonically clean it with ethanol and double distilled water, cut off a 3cm length after complete drying, and then bond it to the 0.1mm copper wire end, placed in In the 1.0mm glass capillary, the carbon fiber electrode is sealed in the glass capillary on the glass capillary puller, and the copper wire exposed at the other end of the glass capillary is sealed and fixed with epoxy resin; then the carbon fiber electrode is immersed in phenol and 2-allyl In the base phenol solution, ...

Embodiment 2

[0052] Embodiment 2: gold / platinum (Au / Pt) ultramicro combined electrode

[0053] The preparation process of gold / platinum (Au / Pt) ultrafine composite electrode is similar to that of Example 1. After confirming the various materials and methods used, at first the gold wire with a diameter of 5nm is cleaned in nitric acid and double distilled water, dried and used The conductive adhesive is bonded to the copper wire, placed in a capillary to draw and seal, and the surface of the gold wire is coated with insulating varnish. After drying, an insulating layer with a thickness of 120nm is obtained, and then a layer of 50nm thick platinum is sputtered on the outside of the insulating layer. , using a copper wire to lead out as an alignment / quasi-reference counter electrode, and finally, the front end of the gold electrode coated with a platinum layer and an insulating layer is cut vertically to obtain a gold / platinum (Au / Pt) ultramicro composite electrode. Using the same treatment m...

Embodiment 3

[0055] Embodiment 3: carbon fiber / platinum (CF / Pt) ultramicro composite electrode;

[0056]The cleaning treatment of the carbon fiber is the same as that of Example 1. A layer of epoxy resin is uniformly coated on the clean carbon fiber surface, and the thickness of about 450nm is about 450nm after complete drying. A layer of 50nm thick platinum is sputtered on the epoxy surface, and a copper wire is used to draw it as To align / quasi-reference the counter electrode, finally, the front end of the carbon fiber electrode coated with the platinum layer and the epoxy insulating layer is cut vertically to obtain a carbon fiber / platinum (CF / Pt) ultramicro composite electrode. Using the same treatment method as above, after the insulating layer is formed on the surface of the carbon fiber electrode, the front end of the electrode is cut off directly, and the platinum layer is no longer sputtered, that is, the carbon fiber disk electrode is obtained.

[0057] The carbon fiber / platinum ...

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Abstract

The invention provides an electrochemical ultramicro electrode preparation process, which combines an ultramicro working electrode and an alignment / quasi-reference electrode to form a two-electrode system, and separates the two electrodes with a nanometer-thick insulating layer. After the diffusion layers on the two electrodes overlap, the electrochemically reversible particles are oxidized or reduced on one electrode, regenerated by reduction or oxidation on the other electrode, and then diffuse back to the original electrode, so that the probe cell The current signal output on the electrodes is amplified. The invention can significantly reduce the resistance of the solution, greatly improve the available scanning speed and detection sensitivity of the actual system analysis, and minimize the damage to the research system. The combined electrode is easy to manufacture, has a long life and is easy to update. Chemical biosensors can be used for high-spatial-resolution dynamic detection of single-cell release and real-time dynamic analysis of single vesicles in cells.

Description

【Technical field】 [0001] The invention relates to the technical fields of electroanalytical chemistry and biosensor, in particular to an ultramicro electrode combination method, an ultramicro combined electrode structure made according to the method, and a process method for making the electrode. 【Background technique】 [0002] Cell is the basic unit of biology, the starting point of evolution, and the basic object that life science must understand. Develop new technologies for intracellular detection, and detect neurotransmitters such as dopamine (Dopamine, DA), nitric oxide (NO), hydrogen peroxide (H 2 o 2 ) and oxygen free radicals and other information molecules in vivo, real-time and dynamic chemical detection is a very challenging frontier topic in analytical chemistry, and it is also an important entry point for in-depth research on the functions of the brain and nervous system. [0003] The detection techniques of intracellular neurotransmitters and information mol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
Inventor 蒋晓华陈建军刘伟强朱永法
Owner SAMXON ELECTRONICS DONG GUAN
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