Method for preparing Chinese yam saponin by microorganism transformation process

A technology for microbial transformation and diosgenin, which is applied to the field of microbial transformation of diosgenin in plants into diosgenin, can solve the problems of increased production cost, expensive equipment and operating costs, large waste water treatment investment, and the like, and achieves reduction of raw materials. The effect of waste, lower production costs, and higher yields

Inactive Publication Date: 2007-08-08
DALIAN UNIV OF TECH
View PDF10 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In recent years, improved chemical preparation methods of diosgenin include ethanol crystallization (CN1188425C), super (near) critical water hydrolysis (CN1896093), vaporized acid acid hydrolysis (CN1709904), and the use of new equipment (CN1182152C) for production, but these methods all can't get rid of the acid hydrolysis process, although there are also the disclosure of the patents of the non-wastewater production method (CN1850852) and the pollution-free production method (CN1850853) of diosgenin, the treatment cost of waste water and pollutants is huge, and will undoubtedly increase production cost
The supercritical fluid extraction method disclosed in Chinese patent CN1240791 does not use an acid hydrolysis process, but the equipment and operating costs are expensive,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing Chinese yam saponin by microorganism transformation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Medium: A. Seed medium: bran extract (10g bran plus 100mL tap water boiled for 20min, filtered, and the filtrate was added to 100mL with water) 30mL. B. Fermentation medium: 0.9g (60 mesh) of Dioscorea scutellaria powder, 0.1g of urea, KH 2 PO 4 0.03g, bran extract 30mL, sterilized at 121°C for 20min.

[0023] The slant surface of Aspergillus oryzae CICC 2436 was inoculated on the seed culture based on 25°C for 24 hours. The fermentation culture was carried out in a 250mL shake flask, the actual liquid volume was 30mL, the inoculation amount was 10%, the speed was controlled at 150rpm, and the temperature was controlled at 30°C . After the fermentation was carried out for 48 hours, the culture temperature was changed to 50° C., and the culture was continued for 2 hours.

[0024] After the cultivation, add 95% ethanol twice the volume of the fermentation broth, microwave-assisted extraction, add 1% medical activated carbon for decolorization, evaporate the ethanol, e...

Embodiment 2

[0027]Medium: A. Seed medium: 30mL of bran extract. B. Fermentation medium: transformation substrate pangolin powder 0.9 g (60 mesh), urea 0.08 g, KH 2 PO 4 0.03g, bran extract 30mL, sterilized at 121°C for 20min.

[0028] The slant surface of Aspergillus oryzae CICC 2436 was inoculated on the seed culture based on 25°C, 24 hours of cultivation, the fermentation culture was carried out in a 250mL shake flask, the actual liquid volume was 30mL, the inoculation amount was 8%, the speed was controlled at 220rpm, and the temperature was controlled at 25°C After 60 hours of fermentation, the culture temperature was changed to 60° C., and the culture was continued for 3 hours.

[0029] After the cultivation, add 80% methanol twice the volume of the fermentation broth, microwave-assisted extraction, add 1% medical activated carbon for decolorization, evaporate ethanol, extract with an appropriate amount of No. 120 gasoline, concentrate, and use an appropriate amount of 90 The pur...

Embodiment 3

[0032] Culture medium: A. Seed medium: bran extract. B. Fermentation medium: 2500mL of bran extract, 75g of transformation substrate Dioscorea scutellaria, 7.5g of urea, KH 2 PO 4 3g, sterilized at 121°C for 20min.

[0033] The slant surface of Aspergillus oryzae CICC 2436 was inoculated on the seed culture based on the seed medium, the liquid volume was 30mL, the shaking table speed was 220rpm, the culture temperature was 30°C, and the culture time was 24 hours; the fermentation culture was carried out in a 5L fermenter, and the actual loading The liquid volume is 2.5 L, the inoculum volume is 10%, the rotational speed is controlled at 250 rpm, the air volume is 1 vvm, and the temperature is controlled at 30°C. After the fermentation was carried out for 60 hours, the culture temperature was changed to 50° C., and the culture was continued for 2 hours.

[0034] After the fermentation is finished, add 95% ethanol 1 times the volume of the fermentation broth, and microwave-a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Melting pointaaaaaaaaaa
Melting pointaaaaaaaaaa
Melting pointaaaaaaaaaa
Login to view more

Abstract

The invention discloses a transmitting method from dioscin into diosgenin saponin in the biological conversing and purifying technical domain of natural product, which comprises the following steps: adding carbon source and nitrogen source to form seed culture medium and fermenting culture medium; seeding aspergillus oryzae; aerating gas to stir; fermenting; extracting ferment liquid assisted by microwave; improving receiving rate by 1.8-2.2 times corresponding to acid hydrolytic method.

Description

technical field [0001] The invention belongs to the technical field of biotransformation and extraction of natural products, and particularly relates to a method for converting diosgenin in plants into diosgenin with microorganisms. Background technique [0002] Diosgenin (Formula 1), which is a derivative of isospirostane, is a diosgenin in the rhizome of Dioscorea genus Dioscorea, and its glycosides are usually glucose, rhamnose, galactose, arabinose, etc. Dioscin has the effect of estrogen, which can lower cholesterol, relieve cough, eliminate phlegm, desensitize, restore diseased tissue, and stimulate heparin cells and bile secretion. It is an ideal precursor for the synthesis of various steroid hormones and steroidal contraceptives. [0003] Dioscorea saponins widely exist in plants in nature, especially in Dioscoreaceae. Among them, Dioscorea zingiberensis wright, D. nipponica Makino, D. parviflora Ting Sp. Nov, D. Panthaica Prainet. .nipponica Makno subsp. Deltoide...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P33/20C12R1/69
Inventor 修志龙董悦生刘琳齐珊珊王辉张代佳
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap