Medicine for controlling livestock-poultry virus infectious disease
A viral and infectious disease technology, applied in antiviral agents, pharmaceutical formulas, plant raw materials, etc., can solve problems such as vaccine immune failure, immune effect cell immunity deficiency, toxicity, etc., achieve obvious therapeutic effects, and promote lymphocyte proliferation activity Effect
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Embodiment 1
[0016] Example 1: Experiment of cell inhibition of animal virus
[0017] VSV was used to detect the drugs provided by the present invention on MDBK, PK, and chicken fibroblasts respectively. Multiple test results show that the drug provided by the present invention has high antiviral activity, which is 100-1000 times higher than the simple interferon group on average.
[0018] Swine fever virus is cultured on PK cells, then stained with swine fever fluorescent antibody, and observed by fluorescence microscope. The result shows that the drug provided by the present invention has 100 times higher activity than simple interferon.
[0019] Cultivate PK cells and Marc145 cells in DMEM medium on 96-well micro cell plates. After they grow into cell monolayers, they are treated with different contents of the product of the present invention, and then aspirated and then inoculated with 1000 TCID50 porcine epidemic diarrhea. Porcine Transmissible Gastroenteritis Virus, Porcine Reproductive ...
Embodiment 2
[0021] Example 2: Clinical experiment
[0022] Clinically with the drugs of the present invention:
[0023] Prevention and treatment of swine fever, swine epidemic diarrhea, swine transmissible gastroenteritis, swine reproductive and respiratory disorder syndrome, swine circovirus disease, swine foot-and-mouth disease, swine parvovirus disease, swine Japanese encephalitis, swine pseudorabies, Swine flu. The use method of the medicine provided by the present invention: 0.01ml injection per kilogram of body weight is diluted with an appropriate amount of sterile normal saline and injected intramuscularly. Each milliliter of injection contains 1 million units of porcine interferon and 100ug of pig-specific serum antibodies. One injection, two injections during the onset of the disease. Used in pregnant sows to protect the fetus from viral infections.
[0024]Prevention and treatment of chicken Newcastle disease, bursa of fabric, infectious bronchitis, infectious laryngotracheitis, en...
Embodiment 3
[0026] Example 3: The medicine provided by the present invention can be made into liquid and powder according to convenient clinical use. The auxiliary components of the liquid also include EDTA, mercaptoethanol, tris-HCL, MgCL 2 , KCL and 10%-50% glycerin, and adjust the PH value of 7.0-7.4. Store at low temperature below -18℃, and transport at 0-4℃. The auxiliary components of the powder also include skimmed milk powder, glucose, sucrose, starch, polylactose, polyethylene glycol, and carboxymethyl cellulose.
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