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Medicine for controlling livestock-poultry virus infectious disease

A viral and infectious disease technology, applied in antiviral agents, pharmaceutical formulas, plant raw materials, etc., can solve problems such as vaccine immune failure, immune effect cell immunity deficiency, toxicity, etc., achieve obvious therapeutic effects, and promote lymphocyte proliferation activity Effect

Inactive Publication Date: 2007-10-24
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, there are several methods for the prevention and treatment of viral infectious diseases of livestock and poultry: 1. Immunization and vaccination: Many viral infectious diseases of animals are well prevented and controlled through vaccination, but there are still some serious problems: ①Attenuated virus There are potential safety hazards in the vaccine; ②Although the inactivated vaccine is safe, the immune effect, especially the cellular immunity, is limited; ③Although the genetically engineered subunit vaccine has the potential, its immune effect, especially the cellular immunity, is insufficient; ④In broiler chicken production Among them, the injection of oil-emulsion adjuvant inactivated vaccine will cause body residues and seriously affect the quality of meat
⑤ The serotype of the disease tends to be complex and the strains mutate rapidly, which often leads to the failure of vaccine immunity; ⑥ New viruses are constantly emerging, and there is no successful vaccine for vaccination at present, which is difficult to control for a while
It has certain toxicity to the body cells and cannot be used for a long time. Long-term use will lead to drug resistance and affect the clinical effect of antiviral drugs
3. Antiviral Chinese herbal medicine: Antiviral Chinese herbal medicine has played an effective role in the treatment and prevention of viral infectious diseases in livestock and poultry. Many farmers add a large amount of antiviral drugs to animal feed or drinking water to prevent infectious diseases, but antiviral The use of Chinese herbal medicines in feed and drinking water leads to the residual smell of Chinese herbal medicines in the body, which seriously affects the export of meat products in my country
In addition, the antiviral active ingredients of Chinese herbal medicine and their mechanism have not been studied clearly, which is a technical obstacle for the use of Chinese herbal medicine in the prevention and treatment of viral infectious diseases of livestock and poultry, which has not been recognized by most countries.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Experiment of cell inhibition of animal virus

[0017] VSV was used to detect the drugs provided by the present invention on MDBK, PK, and chicken fibroblasts respectively. Multiple test results show that the drug provided by the present invention has high antiviral activity, which is 100-1000 times higher than the simple interferon group on average.

[0018] Swine fever virus is cultured on PK cells, then stained with swine fever fluorescent antibody, and observed by fluorescence microscope. The result shows that the drug provided by the present invention has 100 times higher activity than simple interferon.

[0019] Cultivate PK cells and Marc145 cells in DMEM medium on 96-well micro cell plates. After they grow into cell monolayers, they are treated with different contents of the product of the present invention, and then aspirated and then inoculated with 1000 TCID50 porcine epidemic diarrhea. Porcine Transmissible Gastroenteritis Virus, Porcine Reproductive ...

Embodiment 2

[0021] Example 2: Clinical experiment

[0022] Clinically with the drugs of the present invention:

[0023] Prevention and treatment of swine fever, swine epidemic diarrhea, swine transmissible gastroenteritis, swine reproductive and respiratory disorder syndrome, swine circovirus disease, swine foot-and-mouth disease, swine parvovirus disease, swine Japanese encephalitis, swine pseudorabies, Swine flu. The use method of the medicine provided by the present invention: 0.01ml injection per kilogram of body weight is diluted with an appropriate amount of sterile normal saline and injected intramuscularly. Each milliliter of injection contains 1 million units of porcine interferon and 100ug of pig-specific serum antibodies. One injection, two injections during the onset of the disease. Used in pregnant sows to protect the fetus from viral infections.

[0024]Prevention and treatment of chicken Newcastle disease, bursa of fabric, infectious bronchitis, infectious laryngotracheitis, en...

Embodiment 3

[0026] Example 3: The medicine provided by the present invention can be made into liquid and powder according to convenient clinical use. The auxiliary components of the liquid also include EDTA, mercaptoethanol, tris-HCL, MgCL 2 , KCL and 10%-50% glycerin, and adjust the PH value of 7.0-7.4. Store at low temperature below -18℃, and transport at 0-4℃. The auxiliary components of the powder also include skimmed milk powder, glucose, sucrose, starch, polylactose, polyethylene glycol, and carboxymethyl cellulose.

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PUM

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Abstract

The invention relates to a medicine which is used to prevent and treat livestock virus infection, comprising interferon, specific antibody and effective extract. The medicine is characterized by wide anti-virus effect, fast curative effect, small medicine consumption and reduced irritated response caused by capturing livestock for injection. The medicine can enter into embryo through placenta and prevent virus copy in the embryo, so it can be used for pregnant livestock. The progress is dramatic, and it overcomes shortcoming that other anti-virus medicine can not enter into embryo through placenta and protect embryo.

Description

Technical field [0001] The invention relates to a medicine, in particular to a medicine for preventing and treating viral infectious diseases of livestock and poultry, belonging to the field of biotechnology. Background technique [0002] Livestock and poultry viral infectious diseases are the biggest threat to the breeding industry, causing serious economic losses to the breeding industry. Traditionally, the prevention and treatment of viral infectious diseases in livestock and poultry has the following methods: 1. Immunization: Many animal viral infectious diseases are well prevented through vaccination, but there are still some serious problems: ① Attenuated Vaccines have potential safety hazards; ②Although inactivated vaccines are safe, their immune efficacy, especially cellular immunity, has limitations; ③Although genetically engineered subunit vaccines have potential, their immune effects, especially cellular immunity, are insufficient; ④produced in broilers Among them, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P31/12A61K38/21A61K36/899
CPCY02A40/70
Inventor 王彦彬崔保安康相涛许兰菊
Owner HENAN AGRICULTURAL UNIVERSITY
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