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Target polypeptide-gold/silicon dioxide nano complex particle and synthesis thereof

A nano-composite particle, silicon dioxide technology, which is applied to inactive components of polymer compounds, drug combinations, digestive systems, etc., can solve the problem of insignificant changes and achieve the effect of enhancing specific targeting.

Inactive Publication Date: 2009-01-28
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter how these pure gold particles change their particle size, the maximum ultraviolet absorption or scattering can only be below 520nm, and the degree of change is not obvious.

Method used

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  • Target polypeptide-gold/silicon dioxide nano complex particle and synthesis thereof
  • Target polypeptide-gold/silicon dioxide nano complex particle and synthesis thereof
  • Target polypeptide-gold/silicon dioxide nano complex particle and synthesis thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Synthesis of nano-gold core-shell particles with a particle size of about 180nm

[0043] (1) Synthesis of silica-based cores in core-shell particles

[0044] Add 50mL of absolute absolute ethanol and 3.0mL of ammonia (25%) into a 100mL clean beaker. Under stirring conditions, add 0.2mL (0.9mmol) of 99.999% pure ethyl orthosilicate to the solution. (TEOS), the solution becomes milky white turbid after about 15~30mim, continue to stir for 8h. Silica particles with an average particle diameter of 140 nm were obtained. Measure the surface charge and pH value of the solution with a dynamic light scattering instrument. By adjusting the concentration of ammonia and the amount of TEOS, spherical silica particles with a particle size of 50-300 nm can be synthesized.

[0045] (2) Ammonification of silica surface

[0046] The particle suspension obtained in the above experiment was centrifuged (2000 rpm), the supernatant was discarded, and the precipitate was added with 10 ...

Embodiment 2

[0053] Example 2: Synthesis of targeting polypeptide-gold / silica nanocomposite particles using sulfhydryl-containing amino acids as link arms

[0054] (1) Protection of cysteine ​​sulfhydryl group

[0055] To a 50mL mixed solvent of cysteine ​​(1.21g, 0.01mol) and triethylamine (1.01g, 0.01mol) in dry dichloromethane (DCM) and N,N-dimethylformamide (DMF) (Volume ratio 9:1) Add triphenylchloromethane (2.78g, 0.01mol) dropwise to the middle, under the protection of nitrogen, after 24 hours of reaction, the most of the solvent is evaporated under reduced pressure, and 10mL of distilled water is added to the residue After ultrasonic dispersion, centrifugal separation, discard the supernatant, and vacuum dry the obtained solid to obtain thiol-protected cysteine.

[0056] (2) Introduce the sulfur-containing group-sulfhydryl group into the targeted dodecapeptide through cysteine

[0057] Fix the amine group of the sulfhydryl-protected cysteine ​​(C) on the resin, and use commercial solid...

Embodiment 3

[0060] Example 3: Synthesis of targeting polypeptide-gold / silica nanocomposite particles with sulfhydryl-containing polyethylene glycol (PEG) as the linking arm

[0061] (1) Functional modification of PEG

[0062] The first step: introducing halogenated groups to both ends of PEG. Take PEG2000 (number average molecular weight 2000, 10g, 5mmol) and dissolve it in toluene (250mL), and azeotropically remove water from the oil-water separator. Add freshly distilled pyridine (0.41 mL, 5 mmol) and thionyl chloride (3.54 mL, 50 mmol), and reflux at 75°C for 12 h. Cool to room temperature, remove most of the solvent by rotary evaporation, dissolve in dichloromethane, dry with anhydrous potassium carbonate for 12h, and filter. The collected filtrate was adsorbed and treated with neutral aluminum oxide (20g, activated at 120°C for 2h), filtered, the filtrate was concentrated and precipitated with ether, the precipitate was collected and dissolved in dichloromethane, reprecipitated with ethe...

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Abstract

The invention relates to the targeting peptide-gold / silicon dioxide nano-composite particles with biological compatibility and a preparation method thereof, belonging to the technical field of nanometer biological medicine of material science. The targeting peptide-gold / silicon dioxide nano-composite particles provided by the invention take silicon dioxide nano particles as core, the surface of which is coated with a gold casing; moreover, the surface of the gold casing is covered with liver cancer targeting dodecapeptide nano-composite nucleocapsid particles. The targeting peptide-gold / silicon dioxide nano-composite particles adopt a molecular cross-linking agent to crosslink the silicon dioxide and a metal layer; the outer layer of a metal casing is connected with the targeting peptide through the sulfur-containing group of a linking arm. The composite nucleocapsid particles are characterized by having maximum ultraviolet absorption and scattering peak in a near-infrared region, having specific targeting to liver cancer cells, being capable of killing liver cancer cells under the irradiation of near-infrared light source and having no biological toxicity proved by biotic experimental results in vitro, thus having important value in the photothermal therapy of various human cancers.

Description

Technical field [0001] The invention relates to a targeted polypeptide-gold / silica nanocomposite particle with biocompatibility, especially a nano core-shell particle with a silica nanoparticle as the core and a gold shell on the surface, which contains a target on the surface of the gold shell. The invention provides composite nanoparticles of polypeptides and a preparation method of the nanocomposite gold / silica core-shell particles, which belong to the technical field of nano-biomedicine of materials science. Background technique [0002] As we all know, solid gold nanoparticles have special optical properties, namely plasmon resonance absorption. This kind of plasmon resonance absorption is caused by the coupling between the electrons in gold and the instantaneous electromagnetic field. The wavelength of this kind of plasmon resonance absorption can be changed by controlling the particle size of the nanoparticles, thereby changing the wavelength of ultraviolet absorption and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/42A61K9/14A61P35/00A61P1/16A61K47/66A61K47/69
Inventor 刘顺英梁重时罗淑芳高峰余家会
Owner EAST CHINA NORMAL UNIV
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