Controllable slow-releasing nano hydroxyapatite with efficient absorption for antalzyme and preparation method thereof

Abstract

The invention relates to an acicular nano hydroxyapatite material of good absorption capability. The absorption content of lysozgme with different concentration (0.2mg / ml-1.0mg / ml) of the material is 2.14 percent to 19.06 percent, with the highest absorption rate of 95 percent above. The slow-release property of the material is controllable with the slow-release time being controlled between 60min and 960min, while the slow-release rate can be controlled between 60 percent and 95 percent. The release speed of the material can be adjusted by changing the type and pH value of desorption solution. The weak crystallizing hydroxyapatite takes a calcic compound and phosphorus compound solution as a raw material which goes through processes of dropping, reaction, ethanol decentralization, stirring or aging at room temperature, and then the material is prepared. The particle diameter of the hydroxyapatite prepared is at a nano level and distributed evenly and the particles thereof are characterized by low crystallinity, large specific surface area, high surface activity and the like. The adsorption of the lysozgme is completed by selecting a phosphate buffer solution with pH being equal to 7.3, based on the electrostatic interaction, and is characterized by short adsorption time and large adsorption quantity. The acicular nano hydroxyapatite material has the advantages of easily available raw materials, low cost and simple operation and the material obtained has good adsorbability and controllable slow-release property, thus the material can be used as a slow-release carrier of basic proteins such as lysozgme, etc.

Description

technical field [0001] The invention belongs to the technical field of bioengineering materials, and relates to a nano-hydroxyapatite material used for the adsorption and sustained release of lysozyme and a preparation method thereof. Background technique [0002] Lysozyme (Lysozyme, LSZ) is a basic globulin composed of 129 amino acids, which can destroy bacterial cell walls and kill bacteria, so it has antibacterial, antiviral and accelerated tissue recovery effects. It has a wide range of applications in the fields of food industry, bioengineering, and medicine, such as food preservation and preservation, tool enzymes for extracting active substances in bacteria, and prevention of dental caries. The occurrence of dental caries is related to the interaction of three factors: dentin, sugar and pathogenic bacteria. Therefore, effectively eliminating the presence of various pathogenic bacteria in the oral cavity is an effective measure to prevent dental caries. In the past, ...

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Problems solved by technology

Calcium phosphate is used as a carrier material to load and release metal ions slowly, but its sintering process in the preparation process makes its crystallinity high and its degradation and absorption capacity are not ideal

The carriers currently used to load lysozyme are all kinds of organic substances. For example, Chinese patent (2003, publication number: CN1413574) discloses a lysozyme sunscreen and whitening cream, and its carrier is a variety of organic substances such as octyl methoxycinnamate. , although organic substances can be hydrolyzed and disappeared in the body to become an ideal carrier for lysozyme, but lysozyme has high water solubility, and when organic substances are used as carriers, excessive release at the initial stage of administration cannot be avoided. Therefore, in the field of biomedical engineering Apps in are restricted

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