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Cephalosporium acremonium corda rhzomorph, production method and uses thereof

A technology of spores and seeds, applied in the field of acremonium, to achieve the effect of guaranteed source, low cost and simple operation process

Inactive Publication Date: 2009-05-13
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the isolation of antibacterial substances from the endophyte Cephalosporium acremonium

Method used

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  • Cephalosporium acremonium corda rhzomorph, production method and uses thereof
  • Cephalosporium acremonium corda rhzomorph, production method and uses thereof
  • Cephalosporium acremonium corda rhzomorph, production method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Separation and purification of Cephalosporium acremonium IFB-E007

[0027] Take fresh and healthy Trachelospermum root, put it into a fresh-keeping bag, seal and mark, and quickly take the sample to the laboratory (20°C), and isolate the endophytic bacteria within 5 hours. Wash the Trachelospermum root with clean water, cut into small pieces of about 1cm, and soak in 75% alcohol for 1min, 1% sodium hypochlorite solution (containing free chlorine>2.5%) for 10min, 75% alcohol for 1min, and then use the double antibody (200IU / mL) Penicillin and 150IU / ml streptomycin) WA medium (20g agar, distilled water 1000mL) plate culture. After the colony grows from the incision, it is transferred to PCA medium (20g potato, 20g carrot, 20g agar, distilled water 1000mL) for the isolation of endophytic fungi. The colony morphology observation, spore morphology and 18S rDNA molecular identification Cephalosporium acremonium, Cephalosporiumacremonium IFB-E007, preservation unit: Gen...

Embodiment 2

[0028] Example 2: Solid fermentation of Cephalosporium acremonium IFB-E007

[0029] Activate the plant endophyte Cephalosporium acremonium (Cephalosporium acremonium IFB-E007), inoculate the fresh mycelium mass into a 1000 mL conical flask, each flask contains 400 mL of PDA medium, cultured at 140 rpm, 28 ℃ for 3 days as Seed liquid: inoculate 20 mL of seed liquid in a jar containing solid medium, and ferment for 30 days at 28°C. The composition of the solid medium is 7.5g of millet, 7.5g of bran, and 0.5 of yeast extract in each bottle. g, FeSO 4 ·7H 2 O 0.01g, sodium tartrate 0.1g, sodium glutamate 0.1g, corn oil 0.1mL, distilled water 15mL.

Embodiment 3

[0030] Example 3: Extraction and Separation of Cephalosporin

[0031] The fermented product obtained in Example 2 was extracted 3 times at room temperature with chloroform / methanol (volume ratio 1:1), with a material-to-liquid ratio of 2:5 (g / ml), filtered and concentrated in a low-temperature vacuum to obtain a black crude extract F; The crude extract F was dissolved in methanol with a material-to-liquid ratio of 7:10 (g / mL). After heating and refluxing for 1 hour, it was cooled to room temperature and placed in a refrigerator at -18°C overnight. Oil and salt substances were repeated three times to obtain extract F1; the extract F1 was subjected to silica gel column chromatography, followed by 10 times the column retention volume of chloroform, chloroform / methanol (volume ratio 100:1), chloroform / methanol ( Volume ratio 100:2), elution with chloroform / methanol (volume ratio 100:4), combine the eluents eluted with chloroform / methanol (volume ratio 100:4) to obtain extract F2; add ...

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Abstract

The invention provides a novel skeleton cephalosol and a method for preparing the novel skeleton cephalosol, wherein the novel skeleton cephalosol has a structural formula shown as the right The novel skeleton cephalosol has strong inhibition effect on Escherichia coli, Pseudomonas fluorescence, Trichophyton rubrum and Candida albican, so the cephalosol can be taken as a compound with antibacterial effect, and is expected to be applied to the preparation of related novel medicines.

Description

Technical field [0001] The present invention relates to a kind of cephalosol extracted and separated from the solid fermentation of the endophytic fungus Cephalosporium acremonium at the root of Trachelospermum jasminoides (Lindl.) Lem. (Apocynaceae)) and the same Preparation method and antibacterial effect. Background technique [0002] Although the secondary metabolites of microorganisms have been widely studied, the special microbes and their active secondary metabolites have not attracted people's attention. Plant endophytes are a special kind of microorganisms with a wide distribution, a wide variety of species, and important physiological and ecological functions. Their active secondary metabolites are becoming a research hotspot in the field of natural product chemistry. [0003] The proliferation of pathogenic bacteria and the enhancement of their drug resistance are a serious threat to human health and life. Antibacterial drugs have been widely used as routine drugs for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K31/366A61P31/04C12P17/18C12R1/75
CPCY02A50/30
Inventor 谭仁祥章华伟黄午阳陈菁蓉谢代前
Owner NANJING UNIV