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Spectrophotometry method for measuring boron content in BNCT medicament in biological samples

A biological sample and spectrophotometric technology, which is applied in the direction of color/spectral characteristic measurement, analysis by causing chemical reaction of materials, material analysis by observing the influence of chemical indicators, etc., can solve the problem of slow reaction speed and large reagent blank , Reduce the difficulty of blanking and other problems, and achieve the effects of short response time, strong specificity, and high sensitivity

Active Publication Date: 2010-05-26
CHINA INSTITUTE OF ATOMIC ENERGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the commonly used curcumin method requires anhydrous state; the methylene blue method has a large reagent blank, and it is difficult to reduce the blank; the anthraquinone method requires a large amount of concentrated sulfuric acid, and the reaction speed is very slow

Method used

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  • Spectrophotometry method for measuring boron content in BNCT medicament in biological samples
  • Spectrophotometry method for measuring boron content in BNCT medicament in biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example is an experiment on the biodistribution of BPA in normal mice. Before the experiment, prepare the following experimental reagents, experimental instruments, configure the required solutions, and process biological samples.

[0016] 1. Experimental reagents

[0017] L-BPA (a kind of BNCT medicine, referred to as BPA), SIGMA company; 3-methoxy-formimine H (98%), Shanghai Jinsheng Chemical Reagent Co., Ltd.; Glacial acetic acid, ammonium acetate, EDTA, concentrated nitric acid , sodium hydroxide, ascorbic acid, and hydrochloric acid were all analytically pure, from Beijing Chemical Reagent Company.

[0018] 2. Experimental equipment

[0019] UV2450 ultraviolet-visible spectrophotometer, Japan SHIMADZU company; ICP-MS, British Isoprobe GB Instrument company; TGL-16L centrifuge, Shanghai Anting Scientific Instrument Factory; BP211D electronic balance, Germany Sartorius company; 211 pH meter, Orion Research; 100-1000 μL pipette, Socorex 1-5 mL pipette, Finnpip...

Embodiment 2

[0035] This example is an experiment on the biodistribution of BPA in nude mice bearing human glioma. The experimental reagents and experimental instruments prepared before the experiment, and the operation of configuring required solutions and processing biological samples are the same as in Example 1.

[0036] 1. Biological sample processing method

[0037] Twenty-one U87MG nude mice with Dutch glioma cells were randomly divided into 7 groups with 3 mice in each group. One group was the blank group without BPA injection, and the other 6 groups were injected with BPA-F injection 100 μL ( Equivalent to 150mg BPA / kg), animals were sacrificed by neck dislocation at 5, 15, 30min, 1, 2, 3 and 6h respectively, blood and tumor, heart, liver, spleen, lung, kidney, stomach, intestine, brain and scalp were collected After homogenizing the tissues, weigh 0.1-0.2 g of each tissue homogenate into a polypropylene tube, take 0.1-0.2 mL of blood, add 0.5-1.0 mL of concentrated nitric acid f...

Embodiment 3

[0046] This example is an experiment on the biodistribution of BPA in normal mice. The preparatory work before the experiment and the steps of measuring the boron content in the sample to be tested by spectrophotometry are the same as in Example 1. The difference is that the pH value of the solution is 6.5, the addition amount of the color developer is 1.6mL, the reaction time is 120min, and the maximum detection wavelength is 410nm.

[0047] It was found that the linearity was good when the boron concentration was 0-5.0 μg / mL. The specific technical solution of the present invention is the technical solution recommended as an example with the final measurement liquid volume being 5mL under normal circumstances. In actual application, the final measurement liquid volume can be adjusted according to the actual situation, and the dosage of other reagents should also be adjusted accordingly.

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Abstract

The invention belongs to the technical field of nuclear chemical analysis and test, in particular to a spectrophotometry method for measuring the boron content in a BNCT medicament in a biological sample. The method comprises the following steps: digesting a biological sample; measuring a standard curve, measuring the biological sample, and the like, wherein a developer is 3-methoxy-azomethine H, the maximum detection wavelength is 390-410nm, and the pH value of final measuring liquid is 4-6.5. The analysis method has the advantages of rapidness, simpleness, convenience, specificity, sensitivity, and the like and satisfies the accuracy and precision requirements for measuring the boron content in the biological sample.

Description

Technical field [0001] The invention belongs to the technical field of nuclear chemical analysis and testing, and specifically relates to a method for measuring the boron content in BNCT drugs in biological samples using spectrophotometry. Background technique [0002] Boron neutron capture therapy (BNCT) is a new type of radiotherapy method that combines 10 Drug B is introduced into the body through oral administration or injection, causing it to selectively accumulate in cancer cells, and then irradiates the lesion with neutrons, causing 10 B occurs 10 B(n, α) 7 Li nuclear reaction, using the resulting alpha particles and 7 Li ions kill cancer cells on a cellular scale. A prerequisite for the success of this method is the accurate measurement of boron concentration in the organism after administration. At present, the main methods for determining the boron content in biological samples include mass spectrometry, atomic spectroscopy, spectrophotometry, electrochemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N21/33
Inventor 李凤林邓新荣樊彩云刘子华罗志福
Owner CHINA INSTITUTE OF ATOMIC ENERGY
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